Structure of PDB 7azs Chain L23A Binding Site BS01

Receptor Information

>7azs Chain L23A (length=95) Species: 300852 (Thermus thermophilus HB8)

MKTAYDVILAPVLSEKAYAGFAEGKYTFWVHPKATKTEIKNAVETAFKVK
VVKVNTLHVRGKKKRLGRYLGKRPDRKKAIVQVAPGQKIEALEGL

Ligand information

>7azs Chain 23SA (length=2889)

gucaagaugguaagggcccacgguggaugccucggcacccgagccgauga
aggacguggcuaccugcgauaagccagggggagccgguagcgggcgugga
ucccuggauguccgaaugggggaacccggccggcgggaacgccggucacc
gcgcuuuuugcgcggggggaaccuggggaacugaaacaucucaguaccca
gaggagaggaaagagaaaucgacucccugaguagcggcgagcgaaagggg
accagccuaaaccguccggcuuguccgggcggggucguggggcccucgga
ccgaauccccagccuagccgaagcuguugggaagcagcgccagagagggu
gaaagccccguaggcgaaagguggggggauaggugaggguacccgaguac
cccgugguucguggagccaugggggaaucugggcggaccaccgccuaagg
cuaaguacuccgggugaccgauagcgcaccaguaccgugagggaaaggug
aaaagaaccccgggaggggagugaaauagagccugaaaccgugggcuuac
aagcagucacggccccgcaagggguuguggcgugccuauugaagcaugag
ccggcgacucacggucgugggcgagcuuaagccguugaggcggaggcgua
gggaaaccgaguccgaacagggcgcaaacggccgaaaaguccgcggccgu
ggacccgaaaccgggcgagcuagcccuggccagggugaagcuggggugag
acccaguggaggcccgaaccggugggggaugcaaaccccucggaugagcu
ggggcuaggagugaaaagcuaaccgagcccggagauagcugguucucccc
gaaaugacuuuagggucagccucaggcgcugacuggggccuguagagcac
ugauagggcuagggggcccaccagccuaccaaacccugucaaacuccgaa
gggucccagguggagccugggagugagggcgcgagcgauaacguccgcgu
ccgagcgcgggaacaaccgagaccgccagcuaaggcccccaagucugggc
uaagugguaaaggauguggcgccgcgaagacagccaggauguuggagcag
ccaucauuuaaagagugcguuagcucacuggucgaguggcgccgcgccga
aaaugaucggggcuuaagcccagcgccgaagcugcgggucugggggauga
ccccaggcgguaggggagcguucccgaugccgaugaaggccgacccgcga
gggcggcuggagguaagggaagugcgaaugccggcaugaguaacgauaaa
gagggugagaaucccucucgccguaagcccaaggguuccuacgcaauggu
cgucagcguaggguuaggcgggaccuaaggugaagccgaaaggcguagcc
gaagggcagccgguuaauauuccggcccuucccgcaggugcgaugggggg
acgcucuaggcuagggggaccggagccauggacgagcccggccagaagcg
cagggugggagguaggcaaauccgccucccaacaagcucugcgugguggg
gaagcccguacgggugacaaccccccgaagccagggagccaagaaaagcc
ucuaagcacaaccugcgggaacccguaccgcaaaccgacacaggugggcg
ggugcaagagcacucaggcgcgcgggagaacccucgccaaggaacucugc
aaguuggccccguaacuucgggagaaggggugcucccuggggugaugagc
cccggggagccgcagugaacaggcucuggcgacuguuuaccaaaaacaca
gcucucugcgaacucguaagaggagguauagggagcgacgcuugcccggu
gccggaaggucaaggggaggggugcaagccccgaaccgaagccccgguga
acggcggccguaacuauaacgguccuaagguagcgaaauuccuugucggg
uaaguuccgaccugcacgaaaagcguaacgaccggagcgcugucucggcg
agggacccggugaaauugaacuggccgugaagaugcggccuacccguggc
aggacgaaaagaccccguggagcuuuacugcagccugguguuggcucuug
gucgcgccugcguaggauaggugggagccugugaacccccgccuccgggu
gggggggaggcgccggugaaauaccacccuggcgcggcugggggccuaac
ccucggauggggggacagcgcuuggcgggcaguuugacuggggcggucgc
cuccuaaaagguaacggaggcgcccaaagguccccucaggcgggacggaa
auccgccggagagcgcaaggguagaagggggccugacugcgaggccugca
agccgagcaggggcgaaagccgggccuagugaaccgguggucccgugugg
aagggccaucgaucaacggauaaaaguuaccccggggauaacaggcugau
cucccccgagcguccacagcggcggggagguuuggcaccucgaugucggc
ucgucgcauccuggggcugaagaaggucccaaggguugggcuguucgccc
auuaaagcggcacgcgagcuggguucagaacgucgugagacaguucgguc
ucuauccgccacgggcgcaggaggcuugaggggggcucuuccuaguacga
gaggaccggaagggacgcaccucugguuucccagcugucccuccaggggc
auaagcuggguagccaugugcggaagggauaaccgcugaaagcaucuaag
cgggaagcccgccccaagaugaggccucccacggcgucaagccgguaagg
acccgggaagaccacccgguggaugggccggggguguaagcgccgcgagg
cguugagccgaccggucccaaucguccgaggucuugacc

<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<
<<<<.....>>>>>>.>>....<<<<<.....<......>......>>>>
>...........<<....>>...<<<<<.....<<.....>>....>>>>
>...(((....<<<<<<<<.....>>>>>>>>......<<<<<<<<<..<
<<...<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<.
....))>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<
<<<<<<<<.......>>>>>>>>>......<<<<<..<<<<>>>>>..))
)......]].>>>>>>.]]...............<<....>>....]]..
.......<<<<....>>>>..................>>>>>>>>>>>..
.....<<<<<<<<<<<....>>>>>>>>>>>...<............>.<
<<<<<....<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...
<<....>>..<<<<.....>>>>.....<<..>>....>>>>>>>>>>>>
>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<.....
.>>>>>>.....>>>...<<<..<<<<........>>>>.>>>....>>>
>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<
....<<<<<....>>>>><<<<<<<<<.....<<<<<<<<<...<<<...
<<<<<<<<.<..<<<<<......>>>>.>..>.>>>>>>>>..>>.>...
>>>>>>>>>.....>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>
>>.....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<
...........<<..<<<<<<<<<........<<<<<.<<...<......
.>...>>.........<<....>>...>>>>>....>>>>>>>>>.>>..
........)))......>>>>>>.>>>...>>>>>>><<<<<<<<.....
>>>>>>>>....>>>>>>..<<<<<...<<<......<<<<<.<<<....
>>>>>>>>...>>>..>>>>>........>>>>>><......<<.....<
<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<..
...>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>
...>>...<<<<<........>>>>>))<<<<<<<<<<<<<...<<<<..
..<<<<<.<<<..<<<<<.<<<<.<<..((((...>>>>>><<<...<<<
<<<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>>>>>>..>>><
...<<<<....>>>>.....>>>>>>...>>>..>>>>>.........>>
>>...>>...>>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.
<<<<<....>>>>>..>>>>..<<<<....<<<<<<<<.<<.........
<.....<<<<<.....<..>......>>>>><<<<<<<<<<<<.....>>
>>>>>>>>>>............<<<<<<<<<..<<<.........>>>..
<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<
<<<<<..<<<...<<...<<<<<....>>>>>...>>...>>>>>>>>..
>>>>><<<<<<.......>>>>>>........<...........<<<<<<
.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.(((.........<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<<<<<<<
<<<<<<<<...........<<.<<..<<<.......<<<<<<<<..>>>>
>>>>....>>>............>>.>>..>>>>>>>>>>>>>>>>...<
<<<<....>>>>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<
<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<......
.>>>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<.....
.>>>..>>>>.<<<....>>>.>>))).......<<<<<<.(((..>>>>
..)))>>>>>>.............>>>>..]]>>>>.......<<<<.<<
<<<<<<<..<<.......>>>..>>>>>>>..>>>>.>.......<<<<<
.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>
>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....
....)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<........
.......>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>
>...>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>
>>>....>>>.>>>>.......>>>>>>>..<<<<.....>>>>....<<
<<<<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>
>>>...>>>..>>>>>.>>>...>>>>....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7azs Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agents.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): M1 K2 S14 E15 K16 K25 H31 T35 K36 T37 E38 K40 N41 E44 K50 K53 N55 T56 L57 H58 R60 K62 K64 R65 L66 Y69 L70 G71 K72 R73 P74 D75
• Binding residue (residue number reindexed from 1): M1 K2 S14 E15 K16 K25 H31 T35 K36 T37 E38 K40 N41 E44 K50 K53 N55 T56 L57 H58 R60 K62 K64 R65 L66 Y69 L70 G71 K72 R73 P74 D75

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7azs, PDBe:7azs, PDBj:7azs
• PDBsum: 7azs
• PubMed: 36827973
• UniProt: Q5SHP0|RL23_THET8 Large ribosomal subunit protein uL23 (Gene Name=rplW)