Structure of PDB 8hl1 Chain L22P Binding Site BS01

Receptor Information

>8hl1 Chain L22P (length=150) Species: 330779 (Sulfolobus acidocaldarius DSM 639)

YPILSIPEDKLAKAVIKNIDASVRDLYNVCKAIRGMNLKEAREFLNNVLE
EKEALPFWRYSHGTSHRSNISRKWKVKSGRYPKKAIKYVLKLLDNAENNA
NSKGLDIDNLKIVHIAAHKGLVLKRYMTRAFGRSTRKYKYLSHIEVILGE

Ligand information

>8hl1 Chain A23S (length=2995)

aagccucccgguggauggcucggcucgggcaccgaagaagggcgcggcaa
gcagcgaaaugcucgggugaggcgcaagcagccguugaccccgagguccc
cuaaugggauauccugccggguuuccggcucccgguuuauaacugggugg
gaaccccccgaacggaaacaucuuaguagggggaggaaaagaaaucaauu
gagauccccugaguaggggcgaccgaaagggggacagcccaaacuaaacc
ugccgaugauaagucgguggggaugugguguuacgaccucuagccugagg
uugaccucggcuuuccuaaccuaucuagccgaacuccccuggaacggggg
gccauagagggugaaagccccguaggcuaaagauagguggaaaguggcua
gagguagaguaccauccccugguuugggggugggaaguuaggggacacgu
gccucuaaggcuaaauaugucccgagaccgauagcaaacuaaguaccgug
agggaaagcugaaaagaaccccggaagggggagugcaaagagccugaaac
cgggugguuauacaggguguggcucgaaagaagugaacccuuccgaagga
aaggggcgcaagcccuuaguacgaggaagggcgaucggggucacgccuuu
cgucuugaaacacgggccggggaguucacaucaguggcgagcuuaaggag
auuaucuccgaaggcauagggaaaccaagugcccgcagccuaguuucuag
gcgaggggcagggucugucagggccugaagccacugaugugaggcuagaa
accgggcgaucuaguccggggcaggcugaaggugggggaaaccccacugg
agggccgaauagggguucugacgugcaauucguucccuugaccucggacu
aggggcaaaagaccaaucuagcccggugauagcuaguuccccccgaaaug
cguccuagcgcagccucccuaaaggcagcucgcgggguagagugacagau
cgggggcuccaggcgccuggggcuuccggucuaacuccgaacccacgagc
gccgaagaaggggggagugggucacucggcguaagguuggguggcaaaag
gggaacaacccagaccuggguuaaggccccaaagucccggcuaagugcca
acgaaaaggcgucuccagccuuagacagcgggaaggugggcccagcagca
gccauccucuaaggagugcguaacagcucacccgccgaggcuggaggccc
uaaagauuggucggggcucaagccgggcgccgagacccaggagggggucu
acuagagauccucugguaggggggcgcugugaugggguagaagguggguc
gugagauccacuggacccgucacaggugcagaucccggcgguaguaacag
cgaaggggggugagaaucccccucgccggaagggcaaggguuucccggca
acguucgucagccgggaguuagccgccuaagguagggccuaauagguacc
uaccgaaagggaaagggguuaacauuccccugccucccggguaggugcgg
uaacgcaagccagacuccugacggauugggguagggagaguaggaccacc
guccuacccaagcacucaagcccuuggagagccguaacggugagaagagg
gcgaaggugugaugggccuuccguuaggaggguucuccugaucccuaguc
cccaugaaaagggagucuggaacgaucccgggagaccguaccuagaaccg
acacuggugcccgggugagaagcccaaggcgucugagggguaacccaggc
uagggaacucggcaaauuagccccguaacuucgggagaaggggugccuau
cgugguuuaacaaagccacgauaggucgcagugaccagagggaccugacu
guuuaauaaaaacauagguccccgcuagcccgaaaggguguguacggggg
cuaaauccuggccacuggugguugaaauccggguucaaccgggcgaagcc
ccaccgaaggccggggguaacucugacccucuuaagguagccaaaugccu
ugccggguaaguuccggcgcgcaugaauggaucaacgcggucccuacugu
cccagccuggggccucgugaacgcccugagccggugcacaguccggcauc
ucccuacaccgagagaagaccccguggagcuucaccgcagccuggcguug
ucccucgggcguuuaugcguagaguaggugggaggggucgaaccuguccu
uucgggggcaggggacccgaaagugaaacaccacccauggacgcucgagg
gacuaaccucucgaaagagaggaacaacgucaggugggcgguucggcugg
ggcggcacucccgcgaaaagauaacacgggagcccaaaggucggcucagg
cgguacagaacgccgccguagagcgcaagggcaaaagccggccugacgug
acccuuccaaguacggggucacgacgcgaaagcggggccuagcgaacgcu
cgugcccccacacgugggggccgggcaugacagaaaaguuaccccgggga
uaacagggucgucgcgggcgagagcucacaucgaccccgcgguuugcuac
aucgaugucggcucuucccacccuggaggugcagcugccuccaaggguag
ggcugcccgcccguuaaaggggagcgugagcuggguuuagaccgucgcga
gacaggucggacucuaaggguagggagugcggaccgcuugaggggaagga
accccuaguacgagaggaacaggguuccggggccuccaguuuaccgguug
uccgguagggcaaugccgggcagccgcgcccugagggguaaccgcugaaa
gcaucuaagcgggaaccccuccccuaaaagaggcggucaggcgccggggc
cuuccccuagaagaggggguugaugggguggggauguaagcuccaagguu
guaagaccgaggaguuuaguccgccacucccaaucaggcuccccc

<.<<<.<<<<<<....<<<..<<<<<<<<<<<<.....<<<<<<<.((..
((.>>>.....<<<<<<...<<<))..)).>>>......>>>>>>...>>
>>...<<<....>>><<<<<<..>>>>>>.<<<<<<.....>>>>>>.>>
...<<<<<<....<<......>>.....>>>>>.>.........<<<..>
>>..<<<<<<.....<<.....>>....>>>>>>..<<<...<<<...<<
<<<<<<<.....>>>>>>>>>.....>>>......<<<<<<<<<<<<<<<
<..>>>>>><<<<<<<..<<<<<<<<.<<<.....<<<<......>>>>.
((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>>>>>
>>>>>......<<<<<<<<......>>>>>>>>......<<<<<.(((((
(>>>>>..>>>......>>.>>>>>>.>>................<<...
.>>....>>.........<<<<.....>>>>............>....>>
>>>>.>>>>......<<<<<<<<<<....<<......<<<<<<<...<..
..<<<<<....>>>>>.....>..>>>>>>>...>>.>>>>>>>>>>..<
<<<........>>>><<<<<<...<<<<<<<<<<<<<<..<<<...<<<<
<...>>>>>...>>>...<<....>>...<<<<<...<<<<.......>>
>>...>>>>><<<<<<....>>>>>>...>>>>>>>>>>>>>>.<<<<..
.<<<<<<.....<<<<<<<<<..<<<<...<<<<<<<....>>>>>>>..
.>.>>>....<<<<<....<<........>>..>>>>>...>>>>>>>>>
...<<.......>>......>>>>>>......>>>>..<<<<<<....<<
<<<....>>>>><<<<<<<<...<<<.<<<<<.<<<...<<<....<<<<
<<<<<<<<<<<<<..>>>>>>>>>>>>>>>>>..>>.>...>>>.>>>>>
>>>......>>>>>>.>>..<<<<<<.<<<.....>>>.>>>>>>....<
<<......>>>...<<<<<<<...<<<(((.....<<<<<<.....<<..
......<<<.<<<<<<<<<<.......<<<<<.<<.....<.........
>.....>>.........<<......>>...>>>>>...>>>>>>>>>>>>
>...........)))>>....>>>>>>.>>>...>>>>>>><<<<<<<.<
....>.>.>>>>>>....>>>>>><<<<<<<<<.<<<.....<<<<<<<<
....>>>>>>>>...>>>>>>>>>>.>>.....>>>>>><......<<..
....<<<<<<.......>>>>>><<<<....<<<<<<<<...<<<<<<..
..<.....>..>>>>>>....<<.{<<...<<<<<<<<<.....>>>.>>
>>>>....>>...<<<<<........>>>>>>><<<<<<<<.....<<<.
...>>>...<<<<<<<<<<...<<<<<<<<<..<<<<<<<<<<<<<.[[[
>>>>>>><<..<<<<<...<<<<<<.....<<<<...>>>>.....>>>>
>>...>>>>>...>><<<<<<<....>>>>>>>>>>>>>...>>>>>>>>
>.........>>>>>>>>>>......>>>>>>>>.}....>>>....<<.
.....>>>>>>><<<<.....>>>>..>>>>...<<<<.....<<<<<<<
<.<<...............<<<<<....<....>.....>>>>><<<<<<
<<<<<<<......>>>>>>>>>>>>>............<<<<<<<<<..<
<<.........>>>..<<<<<<<....<<<<....>>>>......>>>>>
>>..<<<<.<<<..<<<<<<<......<<<<<......>>>>>.......
>>>>>..>>>>>.<<<<<.......>>>>>.........<..........
.<<<<<<.....>>>>>>.....>....>>>>...>>.>>>>>>>.....
>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<
<<<<<<<.<<..<<......<<<<.<<<<<<..<<<<<.<<<<<<<<<.<
<<<<<<<<<<<<<<<<..........<<<<<..<<<......<.<<.<<<
.....>>>>>.>...>>>............>>>>>.>>>>>>>>>>>>>>
>>>...<<<<<<....>>>>>>....>>>>>>>>>..>>>>>...<<<..
...>>>..<<<<<.............>>>>>..<<...<<<<<<<...<<
<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<
<<<<<.........>>>>>>>>>.<<<....>>>.>>))).......<<<
<<.<<<<<..))))))>>>>>>>>>>..........>>>>..>>>>>>..
.....<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>
.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>..<
<<<.....>>>>......>>>>>..>...>>>.>>....<<<<<<<....
>>>.>>>>....>>...>>>>>>>>>>....<<<<<<<..<<<<<..<<<
<<<<.................>>>>>>><<<<<...<.<<...<<<<<<<
<<<]]].>>>>>..>>>>>...>>.>.>>>>>.<<<<<...<<<<<....
.......>>>>>...>>>>>>>>>>......>>>>>>>.<<..>><<<<<
<<<<<<<.......>>>>>....<<<<<<<<<<<<....<<<<<..<<<<
....>>>>..>>>>>...>>>>.>>>>>.>>>...>>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8hl1 Cryo-electron microscopy structure and translocation mechanism of the crenarchaeal ribosome.
• Resolution: 3.93 Å
• Binding residue (original residue number in PDB): K18 V20 K22 R29 D30 Y32 K36 W63 R64 Y65 S66 H67 G68 T69 S70 H71 R72 S73 R77 K80 K82 R85 Y86 K89 N103 N104 H119 A122 H123 K124 R130 T133 R134 A135 F136 G137 R138 T140 R141 K144
• Binding residue (residue number reindexed from 1): K13 V15 K17 R24 D25 Y27 K31 W58 R59 Y60 S61 H62 G63 T64 S65 H66 R67 S68 R72 K75 K77 R80 Y81 K84 N98 N99 H114 A117 H118 K119 R125 T128 R129 A130 F131 G132 R133 T135 R136 K139

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8hl1, PDBe:8hl1, PDBj:8hl1
• PDBsum: 8hl1
• PubMed: 37604686
• UniProt: Q4JB45|RL22_SULAC Large ribosomal subunit protein uL22 (Gene Name=rpl22)