Structure of PDB 7azs Chain L22A Binding Site BS01

Receptor Information

>7azs Chain L22A (length=113) Species: 300852 (Thermus thermophilus HB8)

MEAKAIARYVRISPRKVRLVVDLIRGKSLEEARNILRYTNKRGAYFVAKV
LESAAANAVNNHDMLEDRLYVKAAYVDEGPALKRVLPRARGRADIIKKRT
SHITVILGEKHGK

Ligand information

>7azs Chain 23SA (length=2889)

gucaagaugguaagggcccacgguggaugccucggcacccgagccgauga
aggacguggcuaccugcgauaagccagggggagccgguagcgggcgugga
ucccuggauguccgaaugggggaacccggccggcgggaacgccggucacc
gcgcuuuuugcgcggggggaaccuggggaacugaaacaucucaguaccca
gaggagaggaaagagaaaucgacucccugaguagcggcgagcgaaagggg
accagccuaaaccguccggcuuguccgggcggggucguggggcccucgga
ccgaauccccagccuagccgaagcuguugggaagcagcgccagagagggu
gaaagccccguaggcgaaagguggggggauaggugaggguacccgaguac
cccgugguucguggagccaugggggaaucugggcggaccaccgccuaagg
cuaaguacuccgggugaccgauagcgcaccaguaccgugagggaaaggug
aaaagaaccccgggaggggagugaaauagagccugaaaccgugggcuuac
aagcagucacggccccgcaagggguuguggcgugccuauugaagcaugag
ccggcgacucacggucgugggcgagcuuaagccguugaggcggaggcgua
gggaaaccgaguccgaacagggcgcaaacggccgaaaaguccgcggccgu
ggacccgaaaccgggcgagcuagcccuggccagggugaagcuggggugag
acccaguggaggcccgaaccggugggggaugcaaaccccucggaugagcu
ggggcuaggagugaaaagcuaaccgagcccggagauagcugguucucccc
gaaaugacuuuagggucagccucaggcgcugacuggggccuguagagcac
ugauagggcuagggggcccaccagccuaccaaacccugucaaacuccgaa
gggucccagguggagccugggagugagggcgcgagcgauaacguccgcgu
ccgagcgcgggaacaaccgagaccgccagcuaaggcccccaagucugggc
uaagugguaaaggauguggcgccgcgaagacagccaggauguuggagcag
ccaucauuuaaagagugcguuagcucacuggucgaguggcgccgcgccga
aaaugaucggggcuuaagcccagcgccgaagcugcgggucugggggauga
ccccaggcgguaggggagcguucccgaugccgaugaaggccgacccgcga
gggcggcuggagguaagggaagugcgaaugccggcaugaguaacgauaaa
gagggugagaaucccucucgccguaagcccaaggguuccuacgcaauggu
cgucagcguaggguuaggcgggaccuaaggugaagccgaaaggcguagcc
gaagggcagccgguuaauauuccggcccuucccgcaggugcgaugggggg
acgcucuaggcuagggggaccggagccauggacgagcccggccagaagcg
cagggugggagguaggcaaauccgccucccaacaagcucugcgugguggg
gaagcccguacgggugacaaccccccgaagccagggagccaagaaaagcc
ucuaagcacaaccugcgggaacccguaccgcaaaccgacacaggugggcg
ggugcaagagcacucaggcgcgcgggagaacccucgccaaggaacucugc
aaguuggccccguaacuucgggagaaggggugcucccuggggugaugagc
cccggggagccgcagugaacaggcucuggcgacuguuuaccaaaaacaca
gcucucugcgaacucguaagaggagguauagggagcgacgcuugcccggu
gccggaaggucaaggggaggggugcaagccccgaaccgaagccccgguga
acggcggccguaacuauaacgguccuaagguagcgaaauuccuugucggg
uaaguuccgaccugcacgaaaagcguaacgaccggagcgcugucucggcg
agggacccggugaaauugaacuggccgugaagaugcggccuacccguggc
aggacgaaaagaccccguggagcuuuacugcagccugguguuggcucuug
gucgcgccugcguaggauaggugggagccugugaacccccgccuccgggu
gggggggaggcgccggugaaauaccacccuggcgcggcugggggccuaac
ccucggauggggggacagcgcuuggcgggcaguuugacuggggcggucgc
cuccuaaaagguaacggaggcgcccaaagguccccucaggcgggacggaa
auccgccggagagcgcaaggguagaagggggccugacugcgaggccugca
agccgagcaggggcgaaagccgggccuagugaaccgguggucccgugugg
aagggccaucgaucaacggauaaaaguuaccccggggauaacaggcugau
cucccccgagcguccacagcggcggggagguuuggcaccucgaugucggc
ucgucgcauccuggggcugaagaaggucccaaggguugggcuguucgccc
auuaaagcggcacgcgagcuggguucagaacgucgugagacaguucgguc
ucuauccgccacgggcgcaggaggcuugaggggggcucuuccuaguacga
gaggaccggaagggacgcaccucugguuucccagcugucccuccaggggc
auaagcuggguagccaugugcggaagggauaaccgcugaaagcaucuaag
cgggaagcccgccccaagaugaggccucccacggcgucaagccgguaagg
acccgggaagaccacccgguggaugggccggggguguaagcgccgcgagg
cguugagccgaccggucccaaucguccgaggucuugacc

<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<
<<<<.....>>>>>>.>>....<<<<<.....<......>......>>>>
>...........<<....>>...<<<<<.....<<.....>>....>>>>
>...(((....<<<<<<<<.....>>>>>>>>......<<<<<<<<<..<
<<...<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<.
....))>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<
<<<<<<<<.......>>>>>>>>>......<<<<<..<<<<>>>>>..))
)......]].>>>>>>.]]...............<<....>>....]]..
.......<<<<....>>>>..................>>>>>>>>>>>..
.....<<<<<<<<<<<....>>>>>>>>>>>...<............>.<
<<<<<....<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...
<<....>>..<<<<.....>>>>.....<<..>>....>>>>>>>>>>>>
>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<.....
.>>>>>>.....>>>...<<<..<<<<........>>>>.>>>....>>>
>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<
....<<<<<....>>>>><<<<<<<<<.....<<<<<<<<<...<<<...
<<<<<<<<.<..<<<<<......>>>>.>..>.>>>>>>>>..>>.>...
>>>>>>>>>.....>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>
>>.....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<
...........<<..<<<<<<<<<........<<<<<.<<...<......
.>...>>.........<<....>>...>>>>>....>>>>>>>>>.>>..
........)))......>>>>>>.>>>...>>>>>>><<<<<<<<.....
>>>>>>>>....>>>>>>..<<<<<...<<<......<<<<<.<<<....
>>>>>>>>...>>>..>>>>>........>>>>>><......<<.....<
<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<..
...>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>
...>>...<<<<<........>>>>>))<<<<<<<<<<<<<...<<<<..
..<<<<<.<<<..<<<<<.<<<<.<<..((((...>>>>>><<<...<<<
<<<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>>>>>>..>>><
...<<<<....>>>>.....>>>>>>...>>>..>>>>>.........>>
>>...>>...>>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.
<<<<<....>>>>>..>>>>..<<<<....<<<<<<<<.<<.........
<.....<<<<<.....<..>......>>>>><<<<<<<<<<<<.....>>
>>>>>>>>>>............<<<<<<<<<..<<<.........>>>..
<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<
<<<<<..<<<...<<...<<<<<....>>>>>...>>...>>>>>>>>..
>>>>><<<<<<.......>>>>>>........<...........<<<<<<
.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.(((.........<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<<<<<<<
<<<<<<<<...........<<.<<..<<<.......<<<<<<<<..>>>>
>>>>....>>>............>>.>>..>>>>>>>>>>>>>>>>...<
<<<<....>>>>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<
<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<......
.>>>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<.....
.>>>..>>>>.<<<....>>>.>>))).......<<<<<<.(((..>>>>
..)))>>>>>>.............>>>>..]]>>>>.......<<<<.<<
<<<<<<<..<<.......>>>..>>>>>>>..>>>>.>.......<<<<<
.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>
>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....
....)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<........
.......>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>
>...>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>
>>>....>>>.>>>>.......>>>>>>>..<<<<.....>>>>....<<
<<<<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>
>>>...>>>..>>>>>.>>>...>>>>....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7azs Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agents.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): K4 A5 I6 A7 R8 Y9 R11 S13 R15 K16 R18 R25 N40 K41 R42 Y45 K49 S53 A56 N57 N60 N61 H62 D77 E78 P80 R84 P87 R88 A89 R90 G91 A93 D94 K97 K98 R99 H102
• Binding residue (residue number reindexed from 1): K4 A5 I6 A7 R8 Y9 R11 S13 R15 K16 R18 R25 N40 K41 R42 Y45 K49 S53 A56 N57 N60 N61 H62 D77 E78 P80 R84 P87 R88 A89 R90 G91 A93 D94 K97 K98 R99 H102

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7azs, PDBe:7azs, PDBj:7azs
• PDBsum: 7azs
• PubMed: 36827973
• UniProt: Q5SHP3|RL22_THET8 Large ribosomal subunit protein uL22 (Gene Name=rplV)