Structure of PDB 7r81 Chain L1 Binding Site BS01

Receptor Information

>7r81 Chain L1 (length=217) Species: 5141 (Neurospora crassa)

ARRPARCYRYCKNKPYPKSRFNRGVPDPKIRIFDLGRKRATVDDFPLCIH
MVSNEYEQLSSEALEAARICANKYLVKTAGKEGFHLRVRVHPYHVVRINK
MLSCAGADRLQTGMRGAWGKPNGTVARVNIGQIIMSVRTRDSNKAFALEA
LRRSQYKFPGRQKIIISKNWGFTPLRREEYLEKKAAGKLLIDGAYVQFLG
NRGSLERNMKRFPDAFA

Ligand information

>7r81 Chain A1 (length=3123)

uugaccucggaucagguaggaauacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaacagggauugcccuaguaacggcgagugaagc
ggcaacagcucaaauuugaaaucuggcuucggcccgaguuguaauuugua
gaggaagcuuuuggugaggcaccuucugaguccccuggaacggggcgcca
uagagggugagagccccguauagucggaugccgauccaauguaaagcucc
uucgacgagucgaguaguuugggaaugcugcucaaaaugggagguaaauu
ucuucuaaagcuaaauaccggccagagaccgauagcgcacaaguagagug
aucgaaagaugaaaagcacuuugaaaagaggguuaaauagcacgugaaau
uguugaaagggaagcguuugugaccagacuugcgccguuccgaucauccg
guguucucaccggugcacucgggacggcucaggccagcaucgguuuuggc
ggggggauaaagguccggggaacguagcuccuccgggaguguuauagccc
cgggcguaaugcccucgccgggaccgagguucgcgcaucugcaaggaugc
uggcguaauggucaucaacgacccgucuugaaacacggaccaaggaguca
agguuuugcgcgaguguuuggguguaaaacccgcacgcguaaugaaagug
aacguaggugagagcuucggcgcaucaucgaccgauccugauguauucgg
auggauuugaguaagagcguuaagccuuggacccgaaagauggugaacua
ugcuuggauagggugaagccagaggaaacucugguggaggcucgcagcgg
uucugacgugcaaaucgaucgucaaaucugagcaugggggcgaaagacua
aucgaaccaucuaguagcugguuaccgccgaaguuucccucaggauagca
guguuguucuucaguuuuaugagguaaagcgaaugauuagggaccauucu
caaacuuuaaauauguaagaagcccuuguuacuuaauugaacgugggcau
ucgaauguaccaacacuagugggccauuuuugguaagcagaacuggcgau
gcgggaugaaccgaacgcgggguuaaggugccggaguggacgcucaucag
acaccacaaaaggcguuaguacaucuugacagcaggacgguggccaugga
agucggaauccgcuaaggacuguguaacaacucaccugccgaauguacua
gcccugaaaauggauggcgcucaagcgucccacccauaccccgcccucag
gguagaaacgaugcccugaggaguaggcggccguggaggucagugacgaa
gccuagggcgugagcccgggucgaacggccucuagugcagaucuuggugg
uaguagcaaauacuucaaugagaacuugaaggaccgaaguggggaaaggu
uccaugugaacagcgguuggacauggguuagucgauccuaagccauaggg
aaguuccguuucaaaggggcagugccccguguggcgaaagggaagccggu
uaacauuccggcaccuggauguggguuuugcgcggcaacgcaacugaacg
cggagacgacggcgggggccccgggcagaguucucuuuucuucuuaacgg
ucuaucacccugaaaacaguuugucuggagauaggguuuaauggccggaa
gagcccgacacuucugucggguccggugcgcucucgacgucccuugaaaa
uccgcgggagggaauaauucucacgccaggucguacucauaaccgcagca
ggucuccaaggugaacagccucugguugauagaacaauguagauaaggga
agucggcaaaauagauccguaacuucgggaaaaggauuggcucuaagggu
uggacaaccaacuuagaacugguacggacagggggaaucugacugucuaa
uuaaaacauagcauugcgauggccagaaagugguguugacgcaaugugau
uucugcccagugcucugaaugucaaagugaagaaauucaaccaagcgcgg
guaaacggcgggaguaacuaugacucucuuaagguagccaaaugccucgu
caucuaauuagugacgcgcaugaauggauuaacgagauucccacuguccc
uaucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauc
agcggggaaagaagacccuguugagcuugacucuaguuugacauugugaa
aauuuuuuuacuuauucaauuaagcggggcuggauuuucguccaacuucu
gguuuuaagguccuucgcgggccgacccggguugaagacauugucaggug
gggaguuuggcuggggcggcacaucuguuaaaccauaacgcagguguccu
aaggggggcucauggagaacagaaaucuccaguagaacaaaaggguaaaa
guccccuugauuuugauuuucagugugaauacaaaccaugaaaguguggc
cuaucgauccuuuagucccucgaaauuugaggcuagaggugccagaaaag
uuaccacagggauaacuggcuuguggcggccaagcguucauagcgacguc
gcuuuuugauccuucgaugucggcucuuccuaucauaccgaagcagaauu
cgguaagcguuggauuguucacccacuaauagggaacgugagcuggguuu
agaccgucgugagacagguuaguuuuacccuacugaugaccucaucgcaa
ugguaauugagcuuaguacgagaggaaccgcucauucagauaauugguuu
uugcgguuguccgaccgggcagugccgcgacgcuaccaucugcuggauaa
uggcugaacgccucuaagucagaauccaugccagaacgcgaugauaccuc
cagcacguuguagacguauaagaauaggcuccggccucguaucuuagcag
gcgauuccuccguuggccuugaaguggccagcgguaauucgcguauugua
auuuuaacacgcgcuggaucaaauccuuugcagacgacuuagaugugcga
aaggguccuguaagcaguagaguagccuuguuguuacgaucugcugaggg
uaagcccuccuucgccuagauuu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<..<<.......<<.<<<....>>>.>>.......>>....
<<<<.<<<<<.<<<...<<<<<<..<<....<<<<......>>>>.((..
....<<<.....))>>>....>>..>>.>>>>...>>>.....>>>>>.>
>>>........<<<<<<<........>>>>>>>.....<<<<<..(((((
(>>>>>..>>>..................................<<...
.>>...............<<<<....>>>>....................
..............<<<<.<<<<<<.....<<.<<<<<<<....<....<
<....>>....>........>>>.>>>>.>>.<<<<<<<.<<<<<<<<<<
<<<<<.....<.<<<<<<<<......<<<<<....>>>>>.......>>>
>>>>>....>.>>>>>>>>>>>>>>>........<<<..>>>.....>>>
>>>>.....>>>>>>.>>>>..<<<<........>>>><<<<<<....<<
<<<<<<.<<<<..<<<..<<<<<.....>>>>>..>>>...<<....>>.
......<<<<<..<<....>>...>>>>>.<<<<<<<<...<<....>>.
..>>>.>>>.>>....>>>>.>>>>>>>>..<<.<..<<<<<<<.....<
<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<.
.<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>.
.....>>>>>>...>..>.>>...<<<<<....<.<<<....>>>.><<<
<<<<<<.....<<.<<<<<<<.....<<<....<<<..<.<<.>>.>..>
>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>>.>>>>..
...>>>>...>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>...
.<<<......>>>...<<<<<<<...<<<(((.....<<<<<<......<
..........<<<.<<<<<<<<<<.......<<<<<.<<<...<<.....
....>>...>>>.........<........>...>>>>>...>>>>>>>>
>>>>>..........))).>....>>>>>>.>>>...>>>>>>><<<<<<
<<<.........>>>>>>>>>....>>>>>.<.<<<<<<<<.<<......
<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<
.....<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<..
.<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<<...<<<
....>>>........<<<<<<.>>>>>>....>>>....>>...<<<<<.
.......>>>>>))<<<<.<<<<.<<<....<<<....>>>......(<<
<<<<...<<<<.<<<<<<<<<<<<<.<<<<.......>>>>.><...<<<
<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>...
.><<<<<<<<....>>>>>>>>>>>>.>.>>>>>>>.>>>>.........
>>>>>>.........>>>...>>>>>>>>>>>>..>>.....<<......
>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<..
.......<.....<<<<<....<<..>>.....>>>>><<.<<<<<<<<<
<<..>>>>>..>>>>>>.>>............<<<<<<<<<..<<<....
.....>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>...
<<..<<<..<<<<<<....<<<....<<<<<....>>>>.>...>>>.>>
>>..>>>>><<<<<<.......>>>>>>........<...........<<
<<<.......>>>>>.....>.....>>....>>.>>>>>>>..>..>>.
>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<
<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<<..<<<<<
<....>>>>>>...<<<<<.....<<<<..<<<<......>>>>......
....)...<<<<<.....>>>>>..>>>>..>>>>>.....>>>>>>>>.
.>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<<.
..<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>....
>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)
))......<<<<<<<..<<<<.))))))>>>>>>>>>>>.........>>
>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>>...>>>
>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>
>>>>>...>.<<<.........>>>.....>>>>>..>...>>>.>>...
.<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<<<<<<..
<<<<.<.<<<<<.................>>>>>.><<<<<...<<<<..
..<<<<<<<<<<....>>>>>..>>>>>...>>>>..>>>>>.<<<<...
<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>....<<
<<<<.<<<.<<......<<.......<<<....>>>....>>........
<<<<.....<<<<<<<<<.......>>>>>>>>>....>>>>........
......>>>>>.>>>>>>..<<.<.<<.............>>....<.<<
.<<<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>
....>>>>..>>..>...>.>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7r81 Structure of the translating Neurospora ribosome arrested by cycloheximide
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): R3 R4 P5 A6 R7 C8 K13 N14 K15 P16 R21 F22 D35 K39 R40 E63 A64 R90 H92 Y94 R98 N100 K101 L103 Q112 G114 M115 R116 A118 W119 R154 Y157 K158 P160 R162 D193 G194 Y196
• Binding residue (residue number reindexed from 1): R2 R3 P4 A5 R6 C7 K12 N13 K14 P15 R20 F21 D34 K38 R39 E62 A63 R89 H91 Y93 R97 N99 K100 L102 Q111 G113 M114 R115 A117 W118 R153 Y156 K157 P159 R161 D192 G193 Y195

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7r81, PDBe:7r81, PDBj:7r81
• PDBsum: 7r81
• PubMed: 34815343
• UniProt: Q7S7F0