Structure of PDB 7ls2 Chain L1 Binding Site BS01

Receptor Information

>7ls2 Chain L1 (length=206) Species: 10090 (Mus musculus)

VSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKNYDPQKDKRFSGTVR
LKSTPRPKFSVCVLGDQQHCDERKAVDIPHMDIEALKKLNKNKKLVKKLA
KKYDAFLASESLIKQIPRILGPGLNKAGKFPSLLTHNENMVAKVDEVKST
IKFQMKKVLCLAVAVGHVKMTDDELVYNIHLAVNFLVSLLKKNWQNVRAL
YIKSTM

Ligand information

>7ls2 Chain A2 (length=3706)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccgcgcgucgcggcgugggaaau
guggcguacggaagacccacuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcucgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcguccggccgugccggugguc
cggcggaucuuucccgcgcgggggaccgcccccggccggcgaccggccgc
cgccgggcgcacuuccaccguggcggugcgccgcgaccggcuccgggacg
gccgggaaggcccgguggggaagguggcucggggggggcucaccccgagu
guuacagcccuccggccgcgcuuuucgccgaaucccggggccgaggaaga
ccgucgccgcgcucucccucucccgggugggggccgggccgccccuccca
cggcgcgaccgcuccggggcggacuguccccagugcgccccgggcgucgu
cgcgccgucgggucccgggggcgucggucacgcgugacgaagccgagcgc
acggggucggcggcgaugucggcuacccacccgacccgucuugaaacacg
gaccaaggagucuaacgcgugcgcgagucaggggcucguccgaaagccgc
cguggcgcaaugaaggugaagggccccgcccgggggcccgaggugggauc
ccgaggccucuccaguccgccgagggcgcaccaccggcccgucucgcccg
ccgcgccggggagguggagcacgagcguacgcguuaggacccgaaagaug
gugaacuaugcuugggcagggcgaagccagaggaaacucugguggagguc
cguagcgguccugacgugcaaaucggucguccgaccuggguauaggggcg
aaagacuaaucgaaccaucuaguagcugguucccuccgaaguuucccuca
ggauagcuggcgcucucgcuacgcaguuuuauccgguaaagcgaaugauu
agaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaaaugg
guaagaagcccggcucgcuggcguggagccgggcguggaaugcgagugcc
uagugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaac
gccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaaggug
uugguugauauagacagcaggacgguggccauggaagucggaauccgcua
aggaguguguaacaacucaccugccgaaucaacuagcccugaaaauggau
ggcgcuggagcgucgggcccauacccggccgucgccggcagucggaacgg
aacgggacgggagcggcgucgggccccgcggagccuacgccgcgacgagu
aggagggccgcugcggugagccuugaagccuagggcgcgggcccgggugg
agccgccgcaggugcagaucuuggugguaguagcaaauauucaaacgaga
acuuugaaggccgaaguggagaaggguuccaugugaacagcaguugaaca
ugggucagucgguccugagagaugggcgagugccguuccgaagggacggg
cgauggccuccguugcccucggccgaucgaaagggagucggguucagauc
cccgaauccggaguggcggagaugggcgccgcgaggcguccagugcggua
acgcgaccgaucccggagaagccggcgggagccucggggagaguucucuu
uucuuugugaagggcagggcgcccuggaauggguucgccccgagagaggg
gcccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucuc
gcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggccg
uacccauauccgcagcaggucuccaaggugaacagccucuggcauguugg
aacaauguagguaagggaagucggcaagccggauccguaacuucgggaua
aggauuggcucuaagggcugggucggucgcggccggcgccuagcagccga
cuuagaacuggugcggaccaggggaauccgacuguuuaauuaaaacaaag
caucgcgaaggcccgcggcggguguugacgcgaugugauuucugcccagu
gcucugaaugucaaagugaagaaauucaaugaagcgcggguaaacggcgg
gaguaacuaugacucucuuaagguagccaaaugccucgucaucuaauuag
ugacgcgcaugaauggaugaacgagauucccacugucccuaccuacuauc
cagcgaaaccacagccaagggaacgggcuuggcggaaucagcggggaaag
aagacccuguugagcuugacucuagucuggcacggugaagagacaugaga
gguguagaauaagugggaggcccggccgccggugaaauaccacuacucuc
aucguuuuuucacugacccggugaggcgggggggcgagccccgaggggcu
cucgcuucuggcgccaagcguccgucccgcgcgugcgggcgggcgcgacc
cgcuccggggacagugccagguggggaguuugacuggggcgguacaccug
ucaaacgguaacgcagguguccuaaggcgagcucagggaggacagaaacc
ucccguggagcagaagggcaaaagcucgcuugaucuugauuuucaguacg
aauacagaccgugaaagcggggccucacgauccuucugaccuuuuggguu
uuaagcaggaggugucagaaaaguuaccacagggauaacuggcuuguggc
ggccaagcguucauagcgacgucgcuuuuugauccuucgaugucggcucu
uccuaucauugugaagcagaauucaccaagcguuggauuguucacccacu
aauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuua
cccuacugaugauguguuguugccaugguaauccugcucaguacgagagg
aaccgcagguucagacauuugguguaugugcuuggcugaggagccaaugg
ggcgaagcuaccaucugugggauuaugacugaacgccucuaagucagaau
cccgcccaggcggaacgauacggcagcgccgaggagccucgguuggcccc
ggauagccggguccccguccguccccgcucccgggcgucgggaccggggu
ccggugcggagagccguucgucuugggaaacggggugcggccggaaaggg
ggccgcccucucgcccgucacguugacgcacguucguguggaaccuggcg
cuaaaccauucguagacgaccugcuucugggucgggguuucguacguagc
agagcagcucccucgcugcgaucuauugaaagucagcccucgacacaagg
guuugu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..[[[...<<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>>...<<<<<<<<<....<<<<<<<<.<......<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>..>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<..<<.....<<<<<<<<<<.<<<.<<<.<<<<<.<...
..<<<<.<<<..<<<<<>>>>>>>>>>>>.>>>>>>>>>....>>>.>>>
>>>>>>>......>.>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<<<<
<<...<<.<<<<.<<..<<<......<<<<<<<<.<....>.>>>>>>>>
........>>>>>>>>>.......>>>>>>..>>>>>>>>>>>.......
........<<<<<<.<<<<..<<..>>..>>>>...<<<.<<<.<.<<<<
<<<<<<<<<<<<<..<<<<<<..............>>>>>>.>>>>..>>
>>>>>>>>.>>>....>>>..>>>>.....<<.....>>.....>>>>>>
......>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>>
><<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>..
...>>>...<<....>>...<<<<<<<.....>>>>>>>..<<<<<...<
<<..<<<...(((.[...>>>..>>>....>>>>>.<<<<<<<<<.<<<<
......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<..<<<<<
<<.....<<<<<<<<<..<<<<...<<<<<<<....>>>>>>>...>.>>
>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>...<<..
.....>>......>>>>>>...>..>.>>..<<<<<<....<.<<<....
>>>.><<<<<<<<<<.......<<<.<<<<<<<<<...<<<....<<<..
<.<<<<...<<<<.<<...>>.>>>>.>>>>.>..>>>...>>>...>>>
>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>
.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...
<<<<<<<...<<<(((.......<<<<<.....<<..........<<<.<
<<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>...
......<<......>>...>>>>>...>>>>>>>>>>>>>..........
))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<...........
..].)))...<<.<<.>>>>..................>>>>>>>>>...
.>>>>>>...<<<<<<<..<<......<<<<.<<<<....>>>>>>>>..
.>>.>>>>>>>........>>>>>><<.....<<.....<<<<<<.....
..>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>
>>>....((<<<<<<...<<....<<<....>>>..........<<.<<<
<<<<<<...>>>>>>>>>>>......>>....>>>..<<<<<........
>>>>>))<<<<....<<<<<...<<<<<<<....>>>>>>>...<<<...
.>>>...<..<<<<<<<...<<<<<<<<<<<<<.<<<<<<<<<..{{{..
>>>>>>....<<<<<.<<<<.<<<......<<<.....>>>......>>>
>>>>..>>>>>.....<<<<<<<<<..>>.>>>>>>>>>>..>.>>>>>>
>>>>>>.........>>>>>>>..>..........>>.>>>.>>>>.>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<<
<.....<<<<<<<.<<.........<.....<<<<<....<<..>>....
.>>>>><<.<<<<<<<<<<<..<<<.<<..>>.>>>.......>>>>>..
>>>>>>.>>............<<<<<<<<<..<<<.........>>>..<
<<<<<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<<
<<<.<<..<<<.....<<<<....>>>>.....>>>>>>>>..>>>>><<
<<<<.......>>>>>>........<...........<<<<<.......>
>>>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>
>>>>....>>....<<<<<........>>>>>....[[[[[..{{{.{{.
.....[[[[.{{[[[[..<<<<<..<<<<<<<<.<<<<<<<<<<<<<<<<
<...........<<<<..<<<<.>>>>............>>>>..>>>>>
>>.>>>>>>>>>>...<<<<<.....<<<.<..<.<<<<.<<...>>>>>
>.>..............<<<<<<<.<<<<<....>>>>>>>>>>>>..>>
>>..>>>>>....>>>>>>>>...>>>>>...<<<.....>>>..<<<<<
<...........>>>>>>..<<.....<<<<<...<<<<<<.......>>
>>>>.....<<...(((>>....>>>>>.....<<<.<<<<<.......>
>>>.>>>>.<<<....>>>.>>))).......((((((..(((..>>>>>
>.)))))))))..........]]]]..}}]]]].......<<.<.<.<<<
<<<...<<.......>>...>>>>>>...>>.>.>.......<<<<<.<<
<<<<..<.<.<<<<<......>>>>>...>.>.<<<.........>>>..
...>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}.
.}}}.]]]]].....<<<<<<....<<<..<.<<<<<.............
....>>>>>.><<<<....<<<<....<<.<<<<<<<}}}.>>>>>..>>
.>>...>>>>...>>>><<<<<...<<<<<...........>>>>>...>
>>>>.>>>.....>>>>>>.....<<<<<<.<<<..<<.<....<<<...
.....<<<<<<.<<<<<..<..<.<.........>.>....>..>>>>>>
>>>>>.......>>>...<<<..<<<<......<<.<<<<<<........
>>>>>>>>.....>>>>..>>>...............>.>>..>>>>>>>
>><<<<<.<.............>....<<.<<<<<<<.<<.....<<<<.
...<<<<<......>>>>>...>>>>....>>....>>>>.>>>.>>..>
>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ls2 Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): K24 K27 F28 E33 Q35 R60 K98 L99 K102 K105 C164 A166 R202 Y205 T209 M210
• Binding residue (residue number reindexed from 1): K20 K23 F24 E29 Q31 R56 K94 L95 K98 K101 C160 A162 R198 Y201 T205 M206

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0140236 translation at presynapse
• GO:0140242 translation at postsynapse

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:0045202 synapse
• GO:0098793 presynapse
• GO:0098794 postsynapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ls2, PDBe:7ls2, PDBj:7ls2
• PDBsum: 7ls2
• PubMed: 34815424
• UniProt: P53026|RL10A_MOUSE Large ribosomal subunit protein uL1 (Gene Name=Rpl10a)