Structure of PDB 8to1 Chain L Binding Site BS01

Receptor Information
>8to1 Chain L (length=551) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVM
EEAPAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTR
EGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLIT
GFVDIDPELAREKFAELRAQYVVTRDTIKHATAQEEILKLSEVFKQFRLV
PKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETS
DTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQVKDIN
RRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGL
MKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
LNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIG
DDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMR
FGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLD
D
Ligand information
Receptor-Ligand Complex Structure
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PDB8to1 Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy.
Resolution2.8 Å
Binding residue
(original residue number in PDB)		Y425 K426 T429 W433 R441 R451 P453 H455 V582 R586
Binding residue
(residue number reindexed from 1)		Y363 K364 T367 W371 R379 R389 P391 H393 V520 R524
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0005515 protein binding
GO:0016987 sigma factor activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006355 regulation of DNA-templated transcription
GO:0009408 response to heat
GO:0010468 regulation of gene expression
GO:0045892 negative regulation of DNA-templated transcription
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1903865 sigma factor antagonist complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8to1, PDBe:8to1, PDBj:8to1
PDBsum8to1
PubMed38951624
UniProtP00579|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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