Structure of PDB 8eza Chain L Binding Site BS01

Receptor Information
>8eza Chain L (length=3720) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
AGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSSSPAVLALQT
SLVFSRDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSV
EIKNTCTSVYTKDRAAKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSK
FYGELALKKKIPDTVLEKVYELLGLLGEVHPSEMINNAENLFRAFLGELK
TQMTSAVREPKLPVLAGCLKGLSSLLCNFTKSMEEDPQTSREIFNFVLKA
IRPQIDLKRYAVPSAGLRLFALHASQFSTCLLDNYVSLFEVLLKWCAHTN
VELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYFMEQFYGIIRNVDSN
NKELSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQTDTGDD
RVYQMPSFLQSVASVLLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQL
VCCRAIVKVFLALAAKGPVLRNCISTVVHQGLIRICSKPVVLPTYKDYVD
LFRHLLSSDQMMDSSESLNHLLYDEFVKSVLKIVEKLDLTLEIAANLHPA
KPKDFSAFINLVEFCREILPEKQAEFFEPWVYSFSYELILQSTRLPLISG
FYKLLSITVRNAKKIKYFEGVPEKYSCFALFVKFGKEVAVKMKQYKDELL
ASCLTFLLSLPHNIIELDVRAYVPALQMAFKLGLSYTPLAEVGLNALEEW
SIYIDRHVMQPYYKDILPCLDGYLKTSALEVSALSRAAQKGFNKVVLKHL
KKTKEAISLEEIRIRVVQMLGSLGGQINKNLLTVTSSDEMMKSYVAWDRE
KRLSFAVPFREMKPVIFLDVFLPRVTELALTASDRQTKVAACELLHSMVM
FMLGKATQMPEGGQGAPPMYQLYKRTFPVLLRLACDVDQVTRQLYEPLVM
QLIHWFTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCIREFLKW
SIKQITPQQQEKSPVNTKSLFKRLYSLALHPNAFKRLGASLAFNNIYREF
REEESLVEQFVFEALVIYMESLALAHADEKSLGTIQQCCDAIDHLCRIIE
KKHVSLNKAKKRRLPRGFPPSASLCLLDLVKWLLAHCGRPQTECRHKSIE
LFYKFVPLLPGNRSPNLWLKDVLKEEGVSFLINTFEGGGCGQPSGILAQP
TLLYSLQATLCWLDLLLAALECYNTFIGERTVGALQVLGTEAQSSLLKAV
AFFLESIAMHDIIAAEKSPQEGERYNYSKCTVVVRIMEFTTTLLNTSPEG
WKLLKKDLCNTHLMRVLVQTLCEPASIGFNIGDVQVMAHLPDVCVNLMKA
LKMSPYKDILETHLREKITAQSIEELCAVNLYGPDAQVDRSRLAAVVSAC
KQLHRAGLLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCLPSLDL
SCKQLASGLLELAFAFGGLCERLVSLLLNPAVLSTSVIHFSHGEYFYSLF
SETINTELLKNLDLAVLELMQSSVDNTKMVSAVLNGMLDQSFRERANQKH
QGLKLATTILQHWKKCDSWWAKDSPLETKMAVLALLAKILQIDSSVSFNT
SHGSFPEVFTTYISLLADTKLDLHLKGQAVTLLPFFTSLTGGSLEELRRV
LEQLIVAHFPMQSREFPPGTPRFNNYVDCMKKFLDALELSQSPMLLELMT
EVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLESVYEMFRKDDPRLS
FTRQSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNESTF
DTQITKKMGYYKILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTK
TLIKLCYDAFTENMAGENQLLERRRLYHCAAYNCAISVICCVFNELKFYQ
GFLFSEKPEKNLLIFENLIDLKRRYNFPVMDELNRHECMAPLTALVKHMH
RSLPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPYAKHWL
SPLLQLAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRL
LNFLMKHVFHPKRAVFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDP
NSKDNSVGIQLLGIVMANDLPPYDPQCGIQSSEYFQALVNNMSFVRYKEV
YAAAAEVLGLILRYVMERKNILEESLCELVAKQLKQHQNTMEDKFIVCLN
KVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEVVLCRVEGMTELYFQL
KSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVEFVSHP
STTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENP
GLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEM
TSMSPDYPNPMFEHPLSECEFQEYTIDSDWRFRSTVLTPMGRTDLLRLRR
RFMRDQEKLSLMYARKGVAEQKREKEIKSELKMKQDAQVVLYRSYRHGDL
PDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKEMDKFKTLSEKN
NITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSAGC
LASLQQPVGIRLLEEALLRLLPRLPPDVLRWVELAKLYRSIGEYDVLRGI
FTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKD
FWELASLDCYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLP
YMIRSKLKLLLQGEADQSLLTFIDKAMHGELQKAILELHYSQELSLLYLL
QDDVDRAKYYIQNGIQSFMQNYSSIDVLLHQSRLTKLQSVQALTEIQEFI
SFISKQGNLSSQVPLKRLLNTWTNRYPDAKMDPMNIWDDIITNRCFFLSK
IEEKLTPISSLIRSCKFSMKMKMIDSARKQNNFSLAMKLLKELHKESKTR
DDWLVSWVQSYCRLSHCRSRSQGCSEQVLTVLKTVSLLDENNVSSYLSKN
ILAFRDQNILLGTTYRIIANALSSEPACLAEIEEDKARRILELSGSSSED
SEKVIAGLYQRAFQHLSEAVQAAEEEAAGVIDAYMTLADFCDQQLRKEEE
NASVIDSAELQAYPALVVEKMLKALKLNSNEARLKFPRLLQIIERYPEET
LSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAI
VYPFIISSESYSFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLS
NPELLFKDWSNDVRAELAKTPVNKKNIEKMYERMYAALGDPKAPGLGAFR
RKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDITNMLLLKMNKDSKPPGN
LKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIAGFDERVTVM
ASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDS
ACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYL
SDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVP
ADLLKRAFVRMSTSPEAFLALRSHFASSHALICISHWILGIGDRHLNNFM
VAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLMLPMKETGL
MYSIMVHALRAFRSDPGLLTNTMDVFVKNWYPRQKICYAKRKLAGANPAV
ITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLM
DQATDPNILGRTWEGWEPWM
Ligand information
>8eza Chain R (length=20) Species: 9606 (Homo sapiens) [Search peptide sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
AAAAAAAAAAAAAAAAAAAA
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8eza Cryo-EM visualization of DNA-PKcs structural intermediates in NHEJ.
Resolution4.39 Å
Binding residue
(original residue number in PDB)		P433 K578 K616 K618 D619
Binding residue
(residue number reindexed from 1)		P428 K536 K551 K553 D554
Enzymatic activity
Enzyme Commision number 2.7.11.1: non-specific serine/threonine protein kinase.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003690 double-stranded DNA binding
GO:0003723 RNA binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004677 DNA-dependent protein kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0019899 enzyme binding
GO:0019904 protein domain specific binding
GO:0034511 U3 snoRNA binding
GO:0035979 histone H2AXS139 kinase activity
GO:0044024 histone H2AS1 kinase activity
GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
GO:0106310 protein serine kinase activity
Biological Process
GO:0000460 maturation of 5.8S rRNA
GO:0000723 telomere maintenance
GO:0001756 somitogenesis
GO:0001933 negative regulation of protein phosphorylation
GO:0002218 activation of innate immune response
GO:0002326 B cell lineage commitment
GO:0002327 immature B cell differentiation
GO:0002328 pro-B cell differentiation
GO:0002360 T cell lineage commitment
GO:0006281 DNA repair
GO:0006302 double-strand break repair
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0006310 DNA recombination
GO:0006338 chromatin remodeling
GO:0006468 protein phosphorylation
GO:0006974 DNA damage response
GO:0007420 brain development
GO:0007507 heart development
GO:0008630 intrinsic apoptotic signaling pathway in response to DNA damage
GO:0010212 response to ionizing radiation
GO:0010332 response to gamma radiation
GO:0016233 telomere capping
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0018107 peptidyl-threonine phosphorylation
GO:0019219 regulation of nucleobase-containing compound metabolic process
GO:0030098 lymphocyte differentiation
GO:0031571 mitotic G1 DNA damage checkpoint signaling
GO:0031648 protein destabilization
GO:0032869 cellular response to insulin stimulus
GO:0033077 T cell differentiation in thymus
GO:0033151 V(D)J recombination
GO:0033152 immunoglobulin V(D)J recombination
GO:0033153 T cell receptor V(D)J recombination
GO:0034462 small-subunit processome assembly
GO:0035234 ectopic germ cell programmed cell death
GO:0036211 protein modification process
GO:0042254 ribosome biogenesis
GO:0042752 regulation of circadian rhythm
GO:0043065 positive regulation of apoptotic process
GO:0043066 negative regulation of apoptotic process
GO:0045087 innate immune response
GO:0045621 positive regulation of lymphocyte differentiation
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045727 positive regulation of translation
GO:0045944 positive regulation of transcription by RNA polymerase II
GO:0048511 rhythmic process
GO:0048660 regulation of smooth muscle cell proliferation
GO:0050678 regulation of epithelial cell proliferation
GO:0080135 regulation of cellular response to stress
GO:0097681 double-strand break repair via alternative nonhomologous end joining
GO:0160049 negative regulation of cGAS/STING signaling pathway
GO:1902036 regulation of hematopoietic stem cell differentiation
GO:1905221 positive regulation of platelet formation
GO:2001034 positive regulation of double-strand break repair via nonhomologous end joining
Cellular Component
GO:0000781 chromosome, telomeric region
GO:0000785 chromatin
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005667 transcription regulator complex
GO:0005730 nucleolus
GO:0005829 cytosol
GO:0005958 DNA-dependent protein kinase-DNA ligase 4 complex
GO:0016020 membrane
GO:0032040 small-subunit processome
GO:0032991 protein-containing complex
GO:0032993 protein-DNA complex
GO:0070418 DNA-dependent protein kinase complex
GO:0070419 nonhomologous end joining complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8eza, PDBe:8eza, PDBj:8eza
PDBsum8eza
PubMed37256947
UniProtP78527|PRKDC_HUMAN DNA-dependent protein kinase catalytic subunit (Gene Name=PRKDC)

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