Structure of PDB 7mkj Chain L Binding Site BS01

Receptor Information
>7mkj Chain L (length=471) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
EIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPE
AITYLLEQYDRVEAEEARLSDLITGFVDPELAREKFAELRAQYVVTRDTI
KHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKL
CVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRA
LQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISI
AKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
RSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMP
EDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESL
RAATHDVLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQI
EAKALRKLRHPSRSEVLRSFL
Ligand information
>7mkj Chain P (length=62) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
caaaaagagtattgacttaaagtctaacctataggatacttacagccatc
gagagggacacg
Receptor-Ligand Complex Structure
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PDB7mkj Structural origins of Escherichia coli RNA polymerase open promoter complex stability.
Resolution2.9 Å
Binding residue
(original residue number in PDB)		G106 L110 N383 R385 L386 F419 E420 R423 Y425 K426 S428 T429 Y430 T432 W433 Q437 R451 P453 H455 T583 R586
Binding residue
(residue number reindexed from 1)		G17 L21 N243 R245 L246 F279 E280 R283 Y285 K286 S288 T289 Y290 T292 W293 Q297 R311 P313 H315 T443 R446
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003700 DNA-binding transcription factor activity
GO:0005515 protein binding
GO:0016987 sigma factor activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006355 regulation of DNA-templated transcription
GO:0009408 response to heat
GO:0010468 regulation of gene expression
GO:0045892 negative regulation of DNA-templated transcription
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1903865 sigma factor antagonist complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7mkj, PDBe:7mkj, PDBj:7mkj
PDBsum7mkj
PubMed34599106
UniProtP00579|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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