Structure of PDB 7mke Chain L Binding Site BS01

Receptor Information
>7mke Chain L (length=465) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
IGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEA
ITYLLEQYDRVEAEEARLSDLITGFVELAREKFAELRAQYVVTRDTITAQ
EEILKLSEVFKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCK
MPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQ
IEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTN
RGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQ
ARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRK
VLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHD
VLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALR
KLRHPSRSEVLRSFL
Ligand information
>7mke Chain P (length=44) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
tgttgactattttacctctggcggtgataatgggtactaaggag
Receptor-Ligand Complex Structure
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PDB7mke Structural origins of Escherichia coli RNA polymerase open promoter complex stability.
Resolution3.7 Å
Binding residue
(original residue number in PDB)		M102 R385 L386 K392 K418 R423 Y425 K426 T429 Y430 T432 W433 Q437 R451 H455 R554 V582 T583 E585 R586 Q589 K593
Binding residue
(residue number reindexed from 1)		M12 R239 L240 K246 K272 R277 Y279 K280 T283 Y284 T286 W287 Q291 R305 H309 R408 V436 T437 E439 R440 Q443 K447
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003700 DNA-binding transcription factor activity
GO:0005515 protein binding
GO:0016987 sigma factor activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006355 regulation of DNA-templated transcription
GO:0009408 response to heat
GO:0010468 regulation of gene expression
GO:0045892 negative regulation of DNA-templated transcription
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1903865 sigma factor antagonist complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7mke, PDBe:7mke, PDBj:7mke
PDBsum7mke
PubMed34599106
UniProtP00579|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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