Structure of PDB 6pst Chain L Binding Site BS01

Receptor Information
>6pst Chain L (length=558) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVM
EEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMG
TVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEA
RLSDLITGFVDIDPELAREKFAELRAQYVVTRDTIKHATAQEEILKLSEV
FKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKKNFITL
FTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTI
EQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLI
QEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVH
MIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPI
SMETPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTARE
AKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSE
VLRSFLDD
Ligand information
>6pst Chain O (length=50) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cacaaatccattgacaaaagaaggctaaaagggcatatcctcggcctttg
Receptor-Ligand Complex Structure
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PDB6pst Stepwise Promoter Melting by Bacterial RNA Polymerase.
Resolution3.0 Å
Binding residue
(original residue number in PDB)		E420 R423 Y425 K426 T429 Y430 W434 Q437 R441 R451 P453 H455 E585 R586
Binding residue
(residue number reindexed from 1)		E365 R368 Y370 K371 T374 Y375 W379 Q382 R386 R396 P398 H400 E530 R531
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003700 DNA-binding transcription factor activity
GO:0005515 protein binding
GO:0016987 sigma factor activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006355 regulation of DNA-templated transcription
GO:0009408 response to heat
GO:0010468 regulation of gene expression
GO:0045892 negative regulation of DNA-templated transcription
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1903865 sigma factor antagonist complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6pst, PDBe:6pst, PDBj:6pst
PDBsum6pst
PubMed32160514
UniProtP00579|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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