Structure of PDB 6htq Chain L Binding Site BS01

Receptor Information

>6htq Chain L (length=146) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

MKLHELKPSEGSRKTRNRVGRGIGSGNGKTAGKGHKGQNARSGGGVRPGF
EGGQMPLFQRLPKRGFTNINRKEYAVVNLDKLNGFAEGTEVTPELLLETG
VISKLNAGVKILGNGKLEKKLTVKANKFSASAKEAVEAAGGTAEVI

Ligand information

>6htq Chain A (length=2887)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaug
aaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuug
auccggagauuuccgaauggggaaacccaccacucguaauggagugguau
ccauaucugaauucauaggauaugagaaggcagacccggggaacugaaac
aucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcgg
cgagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugu
aggacacucgaacgagguagaugaagaggucuggaaaggcccgccauagg
agguaacagcccuguagucaaaacuucguucgaguggauccugaguacgg
cggaacacgugaaauuccgucggaauccgggaggaccaucucccaaggcu
aaauacucccuagugaccgauagugaaccaguaccgugagggaaagguga
aaagcaccccggaaggggagugaaagagauccugaaaccgugugccuaca
aguagucagagcccguuaacgggugauggcgugccuuuuguagaaugaac
cggcgaguuacgaucucgugcaagguuaagcagaagaugcggagccgcag
cgaaagcgagucugaauagggcgcaugaguacguggucguagacccgaaa
ccaggugaucuacccauguccagggugaaguucagguaacacugaaugga
ggcccgaacccacgcacguugaaaagugcggggaugagguguggguaggg
gugaaaugccaaucgaaccuggagauagcugguucucuccgaaauagcuu
uagggcuagccucaagguaagagucuuggagguagagcacugauuggacu
aggggccccuaccggguuaccgaauucagucaaacuccgaaugccaauga
cuuauccuugggagucagacugcgagugauaagauccguagucgaaaggg
aaacagcccagaccgccagcuaaggucccaaaguauacguuaaguggaaa
aggauguggaguugcuuagacaaccaggauguuggcuuagaagcagccac
cauuuaaagagugcguaauagcucacuggucgagugacucugcgccgaaa
auguaccggggcuaaacguaucaccgaagcugcggacuguucgaacagug
guaggagagcguucuaagggcugugaagccagaccggaaggacuggugga
gcgcuuagaagugagaaugccgguaugaguagcgaaagaggggugagaau
ccccuccaccgaaugccuaagguuuccugaggaaggcucguccgcucagg
guuagucgggaccuaagccgaggccgaaaggcguaggcgauggacaacag
guugauauuccuguaccaccuccucaccauuugagcaauggggggacgca
ggaggauaggguaagcgcgguauuggauauccgcguccaagcaguuaggc
ugggaaauaggcaaauccguuucccaaaggcugagcugugauggcgagcg
cgaaguuccugauuccacacugccaagaaaagccucuagcgaggugagag
gugcccguaccgcaaaccgacacagguaggcgaggagagaauccuaaggu
gaucgagagaacucucguuaaggaacucggcaaaaugaccccguaacuuc
gggagaaggggugcucuguuagggugcaagcccgagagagccgcagugaa
uaggcccaggcgacuguuuagcaaaaacacaggucucugcgaagccguaa
ggcgaaguauaggggcugacgccugcccggugcuggaagguuaagaggag
cgcuuagcguaagcgaaggugcgaauugaagccccaguaaacggcggccg
uaacuauaacgguccuaagguagcgaaauuccuugucggguaaguuccga
cccgcacgaaaggcgcaacgaucugggcacugucucaacgagagacucgg
ugaaauuauaguaccugugaagaugcagguuacccgcgacaggacggaaa
gaccccguggagcuuuacugcagccugauauugaauguugguacagcuug
uacaggauagguaggagccuuggaaaccggagcgccagcuucgguggagg
caucggugggauacuacccuggcuguauugaccuucuaacccgccgcccu
uaucgggcggggagacagugucaggugggcaguuugacuggggcggucgc
cuccuaaaagguaacggaggcgcccaaagguucccucagaaugguuggaa
aucauucgcagaguguaaaggcacaagggagcuugacugcgagaccuaca
agucgagcagggacgaaagucgggcuuagugauccggugguuccgcaugg
aagggccaucgcucaacggauaaaagcuaccccggggauaacaggcuuau
cucccccaagaguccacaucgacggggagguuuggcaccucgaugucggc
ucaucgcauccuggggcuguagucggucccaaggguugggcuguucgccc
auuaaagcgguacgcgagcuggguucagaacgucgugagacaguucgguc
ccuauccgucgcgggcgcaggaaauuugagaggagcuguccuuaguacga
gaggaccgggauggacgcaccgcugguguaccaguuguucugccaagggc
aucgcuggguagcuaugugcggacgggauaagugcugaaagcaucuaagc
augaagccccccucaagaugagauuucccauuccgcaaggaaguaagauc
ccugaaagaugaucagguugauaggucugagguggaaguguggcgacaca
uggagcugacagauacuaaucgaucgaggacuuaacc

<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<....
..<<<<<<..[..((.>>>.....<<<<<<<..<<<))..]..>>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<....
..>......>>>>>.>.........<<....>>...<<<<......<<..
...>>.....>>>>...(((....<<<<<<<<....>>>>>>>>......
<<<<<<<<..<<<<<<<<.<<<.....<<<<......>>>>.((......
<<<.....))>>>...>>>...>>>>>>>>..>>>>..>>>>........
..<<<.........>>>...........<<<<<..<<<<>>>>>..))).
.....]].>>>>>>>.]]...............<<....>>....]]...
......<<<<....>>>>.............}....>>>>>>>>>>>...
....<<<<..<<<<......>>>>..>>>>...<.<........>.>.<<
<<<<....<<<<<<<.<<<<<..<<<...<<<.....>>>...>>>...<
<....>>..<<<<.....>>>>......>>>>>.>>>>>>>..<<.<..<
<<<<<<.....<<<<<<.<...<<<....<<<<<<......>>>>>>...
..>>>...<<<.<<<<<........>>>>>>>>.....>.>>>>>>...<
<<.....>>>......>>>>>>...>..>.>>..<<<<<<....<<<<<.
...>>>>><<<<<<<<<...<<<<<<<<......<<<...<<<<<<<<..
.<<.<<<<.....>>>>..>>...>>>>>>>>..>>.>......>>>.>>
>>>..>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>>>....<<<
......>>>...<<<<.<<<...<<<(((.....<<<<<<..........
.<<..<<<<<<<<<......<.<<<<<.<<...<<...........>>..
.>>.........<<......>>...>>>>>.>..>>>>>>>>>.>>....
.......))).....>>>>>>.>>>...>>>>>>><<<<<<..>>>>>>.
...>>>>>><.<<<<<<<.<.......<<<<<.<<....>>.>>>>>...
.>.>>>>>>>..>.....>>>>>><......<.....<<<<<<.......
>>>>>><<<<....<<<<<.<<<..<<<<<<...<<.....>>.>>>>>>
....((<<<<.<<...<<<................>>>...>>....<<<
<........>>>>.))<<<<..<<<<<<......<<...<<<<....<<.
<<.<<<..<<<.<<<<<<<<...(((....>>>>>><<..<<<<<..<<<
<<<<<<<<.<<.....>>>>>>>>>...>>>>..>>>>>...>><..<..
>...>>>>>>...>>>...>>>>.........>>>>..>>..>>>>>>.>
>>>>>>>.>>>.....<<......>>>>>>>.<<<<.....>>>>..>>>
>...<<<....<<<<<<<<..<<..............<<<<<........
.......>>>>><<<<<....<<<<....>>>>...>>>>>.........
...<<<<<<<<<..<<<.........>>>..<<<<<<<............
..........>>>>>>>..<<<<.<<<..<<<<<<<..<<<...<<...<
<<<<<.<<....>>..>>>>>>...>>...>>>>>>>>..>>>>><<<<<
<.......>>>>>>........<...........<<<<<.......>>>>
>.....>....>>>>...>>.>>>>>>>....>>..>>>>>>>>.>>>..
>.....>.....<<<<<........>>>>>..<<<<<<<<.(((.[[...
...<<<<.[[<<<<..<<<<<.<<<<<<....<<<.<<<<<<<<<<....
..........<<....<<<......<<<<<<<<....>>>>>>>>...>>
>..............>>.....>>>>>>>>>>.>>>....<<.<<<<<..
.....>>>>>>>........>>>>>>..>>>>>..<<<<.....>>>><<
<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<......
.>>>>>>.....<<.[.(((>>....>>>>>>>...<<<<..<<<.....
.>>>..>>>>.<<<....>>>.>>)))]......<<<<<<<.((..>>>>
..))>>>>>>>.............>>>>..]]>>>>.......<<<<...
<<<<<<...<<.......>>...>>>>>>....>>>.>.......<.<<<
...<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>
>.....>>>>.......>>>..>....<<<<<<<....>>>.>>>>....
]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<........
.......>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.)))>>>>>
>..>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>
>>....>>>.>>>>.......>>>>>>>..<<<<....>>>>....<<<<
<<<<.........>>>>....<<<<<<<<.<<<....<<<<<....>>>>
>...>>>..>>>>>.>>>...>>>>....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6htq Structural Basis for Regulation of the Opposing (p)ppGpp Synthetase and Hydrolase within the Stringent Response Orchestrator Rel.
• Resolution: 4.5 Å
• Binding residue (original residue number in PDB): L3 H4 L6 G11 S12 R13 K14 R16 N17 R18 G20 R21 G22 G24 S25 G28 K29 T30 A31 G32 K33 G34 H35 K36 Q38 N39 A40 R41 S42 G43 R47 E51 G52 G53 Q54 M55 F58 Q59 R60 L61 P62 K63 R64 F66 T67 N68 I69 N70 R71 N78 K81 S103 K104 L112 G113 N114 S129 A130 S131
• Binding residue (residue number reindexed from 1): L3 H4 L6 G11 S12 R13 K14 R16 N17 R18 G20 R21 G22 G24 S25 G28 K29 T30 A31 G32 K33 G34 H35 K36 Q38 N39 A40 R41 S42 G43 R47 E51 G52 G53 Q54 M55 F58 Q59 R60 L61 P62 K63 R64 F66 T67 N68 I69 N70 R71 N78 K81 S103 K104 L112 G113 N114 S129 A130 S131

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6htq, PDBe:6htq, PDBj:6htq
• PDBsum: 6htq
• PubMed: 32937119
• UniProt: P19946|RL15_BACSU Large ribosomal subunit protein uL15 (Gene Name=rplO)