Structure of PDB 3j8g Chain L Binding Site BS01

Receptor Information

>3j8g Chain L (length=143) Species: 83333 (Escherichia coli K-12)

RLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFE
GGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANII
GIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Ligand information

>3j8g Chain B (length=2874)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaa
aaauuagcggaugacuuguggcugggggugaaaggccaaucaaaccggga
gauagcugguucuccccgaaagcuauuuagguagcgccucgugaauucau
cuccggggguagagcacuguuucggcaagggggucaucccgacuuaccaa
cccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacgg
cgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcua
aggucccaaagucaugguuaagugggaaacgaugugggaaggcccagaca
gccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagc
ucacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugc
accgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguuc
uguaagccugcgaaggugugcugugaggcaugcuggagguaucagaagug
cgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgg
aagaccaaggguuccuguccaacguuaaucggggcagggugagucgaccc
cuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccu
guacuugguguuacugcgaaggggggacggagaaggcuauguuggccggg
cgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccg
gaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaac
aaaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaauc
guaccccaaaccgacacagguggucagguagagaauaccaaggcgcuuga
gagaacucgggugaaggaacuaggcaaaauggugccguaacuucgggaga
aggcacgcugauauguaggugaggucccucgcggauggagcugaaaucag
ucgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugc
aaacacgaaaguggacguauacggugugacgccugcccggugccggaagg
uuaauaucgaagccccgguaaacggcggccguaacuauaacgguccuaag
guagcgaaauuccuugucggguaaguuccgaccugcacgaauggcguaau
gauggccaggcugucuccacccgagacucagugaaauugaacucgcugug
aagaugcaguguacccgcggcaagacggaaagaccccgugaaccuuuacu
auagcuugacacugaacauugagccuugauguguaggauaggugggaggc
uuugaaguguggacgccagucugcauggagccgaccuugaaauaccaccc
uuuaauguuugauguucuaacguugacccguaauccggguugcggacagu
gucugguggguaguuugacuggggcggucuccuccuaaagaguaacggag
gagcacgaagguuggcuaauccuggucggacaucaggagguuagugcaau
ggcauaagccagcuugacugcgagcgugacggcgcgagcaggugcgaaag
caggucauagugauccggugguucugaauggaagggccaucgcucaacgg
auaaaagguacuccggggauaacaggcugauaccgcccaagaguucauau
cgacggcgguguuuggcaccucgaugucggcucaucacauccuggggcug
aaguaggucccaaggguauggcuguucgccauuuaaagugguacgcgagc
uggguuuagaacgucgugagacaguucggucccuaucugccgugggcgcu
ggagaacugaggggggcugcuccuaguacgagaggaccggaguggacgca
ucacugguguucggguugucaugccaauggcacugcccgguagcuaaaug
cggaagagauaagugcugaaagcaucuaagcacgaaacuugccccgagau
gaguucucccugacccuuuaaggguccugaaggaacguugaagacgacga
cguugauaggccggguguguaagcgcagcgaugcguugagcuaaccggua
cuaaugaaccgugaggcuuaaccu

<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<......
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<........>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<......>
>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<.<.<<<<<<.....<<<<<<<<<..<
<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<......
..>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>.
..>..>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<
<<<<<<.....<<<...<<<.<<<<...<<.<<<<.....>>>>..>>..
.>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<
<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<..
.<<<(((.....<<<<<<...........<<..<<<<<..<<........
<<<<<.<<...<<<<.......>>>>...>>........<<<......>>
>..>>>>>....>>..>>>>>.>>...........))).....>>>>>>.
>>>...>>>>>>><<<<<<<<<....>>>>>>>>>....>>>>>><<<<<
<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>.>>
.....>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<..
..<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<
<...<<<...<<<....>>>...>>>...>>>..<<<<<........>>>
>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<
....(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>
>>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>
>...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<
<....<<<<<<<<.<<...............<<<<<....<....>....
.>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>
>............<<<<<<<<<..<<...........>>..<<<<<<<..
...<<<....>>>.......>>>>>>>.<<<<<......<<<<<<...<<
<<........>>>>.>>>>..>>....<<<<<.......>>>>>......
...............<<<<<.......>>>>>..........>>>>>..>
>.>>>>>>>.....>>.>>>>>>>>.>>>>>>...>.>.....<<<<<..
......>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<
<..<<<.<<<<<.<<<<<<<<.<<.<.<......................
..................................................
.>..>.>>.>>>>>>>>...<<<.<<<<<<.....>>>>>>>>>....>>
>>>.>>>...>>>>..<<<<.....>>>><<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...((
(>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>
>>.>>))).......<<<<<<<.((..>>>>..))>>>>>>>........
.....>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>
>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.
.....>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....
<<<<<<<<..<<<<..<<<<<<<.....<....>....>>>>>>>.<<<<
<...<<<<...<<<<<<<<<<<))).>>>>>>..>>>>>...>>>>.>>>
>>...<<<....<<<<<...........>>>>>....>>>.>>>>>....
..>>>>>>>..<<<<<<...>>>>>>.....<<..<<<<.........>>
>>....<<<<<<<<..<<....<<<<<....>>>>>...>>...>>>>>.
>>>.....>>.....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3j8g Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly
• Resolution: 5.0 Å
• Binding residue (original residue number in PDB): L3 N4 T5 L6 S7 A9 S12 K13 K14 K17 R18 G20 R21 G22 G24 S25 K29 T30 G31 G32 R33 G34 H35 K36 Q38 K39 R41 S42 R47 R48 E51 G53 Q54 M55 R59 R60 L61 P62 K63 F64 G65 F66 S68 R69 K70 R78 N99 I103 K109 I111 T128 K129 G130
• Binding residue (residue number reindexed from 1): L2 N3 T4 L5 S6 A8 S11 K12 K13 K16 R17 G19 R20 G21 G23 S24 K28 T29 G30 G31 R32 G33 H34 K35 Q37 K38 R40 S41 R46 R47 E50 G52 Q53 M54 R58 R59 L60 P61 K62 F63 G64 F65 S67 R68 K69 R77 N98 I102 K108 I110 T127 K128 G129

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3j8g, PDBe:3j8g, PDBj:3j8g
• PDBsum: 3j8g
• PubMed: 25389271
• UniProt: P02413|RL15_ECOLI Large ribosomal subunit protein uL15 (Gene Name=rplO)