Structure of PDB 3bbx Chain L Binding Site BS01

Receptor Information

>3bbx Chain L (length=144) Species: 562 (Escherichia coli)

MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGF
EGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANI
IGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Ligand information

>3bbx Chain B (length=2902)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaa
aaauuagcggaugacuuguggcugggggugaaaggccaaucaaaccggga
gauagcugguucuccccgaaagcuauuuagguagcgccucgugaauucau
cuccggggguagagcacuguuucggcaagggggucaucccgacuaccaac
ccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacggc
gggugcuaacguccgucgugaagagggaaacaacccagaccgccagcuaa
ggucccaaagucaugguuaagugggaaacgaugugggaaggcccagacag
ccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagcu
cacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugca
ccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguucu
guaagccugcgaaggugugcugugaggcaugcuggagguaucagaagugc
gaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgga
agaccaaggguuccuguccaacguuaaucggggcagggugagucgacccc
uaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccug
uacuugguguuacugcgaaggggggacggagaaggcuauguuggccgggc
gacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccgg
aaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaaca
aaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaaucg
uaccccaaaccgacacagguggucagguagagaauaccaaggcgcuugag
agaacucgggugaaggaacuaggcaaaauggugccguaacuucgggagaa
ggcacgcugauauguaggugaggucccucgcggauggagcugaaaucagu
cgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugca
aacacgaaaguggacguauacggugugacgccugcccggugccggaaggu
uaauugaugggguuagcgcaagcgaagcucuugaucgaagccccgguaaa
cggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgggu
aaguuccgaccugcacgaauggcguaaugauggccaggcugucuccaccc
gagacucagugaaauugaacucgcugugaagaugcaguguacccgcggca
agacggaaagaccccgugaaccuuuacuauagcuugacacugaacauuga
gccuugauguguaggauaggugggaggcuuugaaguguggacgccagucu
gcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaacg
uugacccguaauccggguugcggacagugucugguggguaguuugacugg
ggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaaucc
uggucggacaucaggagguuagugcaauggcauaagccagcuugacugcg
agcgugacggcgcgagcaggugcgaaagcaggucauagugauccgguggu
ucugaauggaagggccaucgcucaacggauaaaagguacuccggggauaa
caggcugauaccgcccaagaguucauaucgacggcgguguuuggcaccuc
gaugucggcucaucacauccuggggcugaaguaggucccaaggguauggc
uguucgccauuuaaagugguacgcgagcuggguuuagaacgucgugagac
aguucggucccuaucugccgugggcgcuggagaacugaggggggcugcuc
cuaguacgagaggaccggaguggacgcaucacugguguucggguugucau
gccaauggcacugcccgguagcuaaaugcggaagagauaagugcugaaag
caucuaagcacgaaacuugccccgagaugaguucucccugacccuuuaag
gguccugaaggaacguugaagacgacgacguugauaggccggguguguaa
gcgcagcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaac
cu

.<<<.<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..<<<..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<......>>>>>..>>>......>>.>>>>>>>.>>.....
..........<<....>>....>>.........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<......>
>>>>..>>>>..................<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<
<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<......
..>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>.
.....>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<
<<<<<<.<...<<<...<<<.<<<<...<...<<.......>>...>...
>>>>.>>>..>>.>...>.>>>>>>.>>>...>>>>>>>.>...<<<<<<
<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<...
<<<(((.....<<<<<<...........<<..<<<<<..<<........<
<<<<.<<...<<<<.......>>>>...>>........<<<......>>>
..>>>>>....>>..>>>>>.>>...........))).....>>>>>>.>
>>...>>>>>>><<<<<<<<<....>>>>>>>>>....>>>>>>..<<<<
<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>....
....>>>>>>..............<<<<<<.......>>>>>><<<<...
.<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<<
...<<<...<<<....>>>...>>>...>>>..<<<<<........>>>>
>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<.
...[[....>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>
>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>>
...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<
....<<<<<<<<.<<.........<.....<<<<<...............
>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
..<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<
<.......<<<<<..<<....>>...>>>>>.......>>>>>>>>>..>
>>>><<<<<<.......>>>>>>........<...........<<<<<..
.....>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>
>>>.>>>>.............<<<<<........>>>>>..<<<<<<<<.
<<<.<<......<<<<.<<<<<<..<<<<<.<<<.<<<<..<<<<<<<<<
<<.<<<<.........>.<<(.[..<<.......<<<<<<<<....>>>>
>>>>....>>............>>).]...>>>.>>>>>>>>>>>...<<
<.<<<<<<.....>>>>>>>>>.....>>>>.>>>..>>>>>..<<<<..
...>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<<
<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<.
.<<<<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<<<
.<<........>>>>>>>>>.............>>>>..>>>>>>.....
..<<<<...<<<<<<...<<.......>>...>>>>>>....>>>.>...
....<<<<<.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<
.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>
.>>>>....>>..>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<
<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<
.]].>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<.....
......>>>>>....>>>.>>>>.......>>>>>>>..<<<<<<...>>
>>>>.....<<..<<<<<.......>>>>>....<<<<<<<<..<<....
<<<<<....>>>>>...>>...>>>>>.>>>.....>>.....>>>.>>>
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3bbx Recycling of Aborted Ribosomal 50S Subunit-Nascent Chain-tRNA Complexes by the Heat Shock Protein Hsp15.
• Resolution: 10.0 Å
• Binding residue (original residue number in PDB): K14 R21 G22 I23 G24 S25 L27 G28 K29 T30 G31 G32 R33 H35 K36 G37 Q38 K39 R41 V46 R47 R48 G49 F50 E51 Q54 M55 L79
• Binding residue (residue number reindexed from 1): K14 R21 G22 I23 G24 S25 L27 G28 K29 T30 G31 G32 R33 H35 K36 G37 Q38 K39 R41 V46 R47 R48 G49 F50 E51 Q54 M55 L79

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3bbx, PDBe:3bbx, PDBj:3bbx
• PDBsum: 3bbx
• PubMed: 19013177
• UniProt: P02413|RL15_ECOLI Large ribosomal subunit protein uL15 (Gene Name=rplO)