Structure of PDB 2otj Chain L Binding Site BS01

Receptor Information

>2otj Chain L (length=145) Species: 2238 (Haloarcula marismortui)

TSKKKRQRGSRTHGGGSHKNRRGAGHRGGRGDAGRDKHEFHNHEPLGKSG
FKRPQKVQEEAATIDVREIDENVTLLAADDVAEFRVDVRDVVEEADDADY
VKVLGAGQVRHELTLIADDFSEGAREKVEGAGGSVELTDLGEERQ

Ligand information

>2otj Chain 0 (length=2754)

uaugccagcugguggauugcucggcucaggcgcugaugaaggacgugcca
agcugcgauaagccauggggagccgcacggaggcgaagaaccauggauuu
ccgaaugagaaucucuaacaauugcuucgcgcaaugaggaaccccgagaa
cugaaacaucucaguaucgggaggaacagaaaacgcaaugugaugucguu
aguaaccgcgagugaacgcgauacagcccaaaccgaagcccucacgggca
auguggugucagggcuaccucucaucagccgaccgucucgacgaagucuc
uuggaacagagcgugauacagggugacaaccccguacucgagaccaguac
gacgugcgguagugccagaguagcggggguuggauaucccucgcgaauaa
cgcaggcaucgacugcgaaggcuaaacacaaccugagaccgauagugaac
aaguagugugaacgaacgcugcaaaguacccucagaagggaggcgaaaua
gagcaugaaaucaguuggcgaucgagcgacagggcauacaaggucccucg
acgaaugaccgacgcgcgagcguccaguaagacucacgggaagccgaugu
ucugucguacguuuugaaaaacgagccagggagugugucugcauggcaag
ucuaaccggaguauccggggaggcacagggaaaccgacauggccgcaggg
cuugcccgagggccgccgucuucaagggcggggagccauguggacacgac
ccgaauccggacgaucuacgcauggacaagaugaagcgugccgaaaggca
cguggaagucuguuagaguugguguccuacaauacccucucgugaucuau
guguaggggugaaaggcccaucgaguccggcaacagcugguuccaaucga
aacaugucgaagcaugaccuccgccgagguagucugugagguagagcgac
cgauugguccugucaaacuccaaacuuacagacgccguuugacgcgggga
uuccggugcgcgggguaagccuguguaccaggaggggaacaacccagaga
uagguuaagguccccaaguguggauuaaguguaauccucugaaggugguc
ucgagcccuagacagccgggaggugagcuuagaagcagcuacccucuaag
aaaagcguaacagcuuaccggccgagguuugaggcgcccaaaaugaucgg
gacucaaauccaccaccgagaccuguccguaccacucauacugguaaucg
aguagauuggcgcucuaauuggauggaaguaggggugaaaacuccuaugg
accgauuagugacgaaaauccuggccauaguagcagcgauagucggguga
gaaccccgacggccuaauggauaaggguuccucagcacugcugaucagcu
gaggguuagccgguccuaagucauaccgcaacucgacuaugacgaaaugg
gaaacggguuaauauucccgugccacuaugcagugaaaguugacgcccug
gggucgaucacgcugggcaucgcccagucgaaccguccaacuccguggaa
gccguaauggcaggaagcggacgaacggcggcauagggaaacgugauuca
accuggggcccaugaaaagacgagcauaguguccguaccgagaaccgaca
cagguguccauggcggcgaaagccaaggccugucgggagcaaccaacguu
agggaauucggcaaguuagucccguaccuucggaagaagggaugccugcu
ccggaacggagcaggucgcagugacucggaagcucggacugucuaguaac
aacauaggugaccgcaaauccgcaaggacucguacggucacugaauccug
cccagugcagguaucugaacaccucguacaagaggacgaaggaccuguca
acggcgggggucuuaagguagcguaguaccuugccgcaucaguagcggcu
ugcaugaauggauuaaccagagcuucacugucccaacguugggcccggug
aacuguacauuccagugcggagucuggagacacccagggggaagcgaaga
cccuauggagcuuuacugcaggcugucgcugaggacucucacuccgggag
gaggacaccgauagccgggcaguuugacuggggcgguacgcgcucgaaaa
gauaucgagcgcgcccuauggcuaucucagccggggacccggcgaagagu
gcaagagcaaaagauagcuugacaguguucuucccaacgaggaacgcuga
cgcgaaagcguggucuagcgaaccaauuagccugcuugaugcgggcaauu
gaugacagaaaagcuacccuagggauaacagagucgucacucgcaagagc
acauaucgaccgaguggcuugcuaccucgaugucgguucccuccauccug
cccgugcagaagcgggcaagggugagguuguucgccuauuaaaggagguc
gugagcuggguuuagaccgucgugagacaggucggcugcuaucuacuggg
uguguaggugucugacaagaacgaccguauaguacgagaggaacuacggu
ugguggccacugguguaccgguuguucgagagagcacgugccggguagcc
acgccacacgggguaagagcugaacgcaucuaagcucgaaacccacuugg
aaaagagacaccgccgaggucccgcguacaagacgcggucgauagacucg
gggugugcgcgucgagguaacgagacguuaagcccacgagcacuaacaga
ccaa

..<<<<<<<<<<<.....((..((<<<<<<.[<<......<<<<<<.((.
.((.>>>.....<<<<<<...<<<))..)).>>>......>>>>>>...>
>>....<<<...>>>.....<<<<<.....>>>>>.>>...<<<<<<...
..<......>......>>>>>.>.........<<.....>>..<<<<<<.
....<<.....>>....>>>>>>..<<<...<<<...<<<<....>>>>.
....>>>....<<<<<<<<......<..<.....<<<<<<<......<<<
<......>>>>.((......<<<.....))>>>....>>>>>>>......
>..>...>>>>>>.>>......<<<<<<<........>>>>>>>......
<<<<<.<<<<<<>>>>>..>>>......]..>>>>>>.))..........
.....<<....>>....))..........<<<.....>>>..........
.........>>>>>>>>>>>.......<<<<<<<<<<....<.<<<<..<
<.........<<<<<....>>>>>.........>>..>>>>...>.>>>>
>>>>>>...<<<........>>>.<<<<<<....<<<<<<<<<<<<<..<
<<...<<<<<...>>>>>...>>>...<<....>>....<<<<<...<<<
<..>>>>...>>>>><<<<<<....>>>>>>...>>>>>>>>>>>>>..<
<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>
>>>.....>>>....<<<..<<.<<........>>>>.>>>....>>>>>
>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<<..
..<<<<<....>>>>><.<<<<<<...<<<.<<<<<<<<<...<<<....
.<<..<<.>>..>>...>>.>...>>>>>>>>>>>>.......>>>>>>.
.>..<<<<<<<<<<.....>>>>>>>>>>....<<<......>>>...<<
<<<<<...<<<(((.....<<<<<<.....<<...........<<<.<<<
<<<<<<<.......<<<<<.<<...<<<<.......>>>>...>>.....
..<<<<......>>>>.>>>>>...>>>>>>>>>>>>>..........))
)>>....>>>>>>.>>>...>>>>>>><<<<<<<.......>>>>>..>>
....>>>>>><...<<<<<<<<......<<<<<<<<....>>>>>>>>..
.>>>>>>>>....>.....>>>>>><<.....<........<<<<<<...
....>>>>>><<<<....<<<<..<<...<<<<<<...<<.....>>.>>
>>>>....((<<<<<<...<<<<<<.<<.....>>..>>>>>>.....>>
>..<<<<<........>>>>>))<<<<<<<<.......<<<...<<<<<<
<<...<<<<<<<<<<<<<.[[>>>>>>><...<<<<<....<<<<<....
<<<<...>>>>.....>>>>>.....>>>>>....>......>>>>>>..
.>>>>>>>>.........>>>..>>>>>>>>.>>>..>>.....<<....
..>>.>>>>..<<<.......>>>..>>>>...<<<<.....<<<<<<<<
.<<.........<.....<<<<<...............>>>>><<<<<<<
<<.....>>>>>>>>>............<<<<<<<<<..<<<........
.>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<
<<..<<<<<<<..<<<.....<<<<......>>>>.....>>>>>>>>..
>>>>><<...>>........<...........<<<<<.......>>>>>.
....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>.>.
...>>.((.<<<<<<......))>>>>>>.<<<<<<<..(((.[[.....
.<<<<.[[<<<<..<<<<<.<<<<<<<<..<<....>>...<<<<....>
>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<.....
......>>>>>>..<<...<<<<<<<...<<<<<<..>>>>>>.....<<
...(((>>....>>>>>>>...<<<<<<<<<.........>>>>>>>>>.
<<<....>>>.>>))).......<<<<<.<<<<<.>>>>>>>>>>>>>>>
>..........>>>>..]]>>>>.......<<<<.<.<<<<<<...<<..
.....>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<
<<<<<......>>>>>>>..>>..<<<<.....>>>>......>>>>>..
>...>>>.>>....<<<<<<<....>>>.>>>>....]]..))).>>>>>
>>....<<<<<<...<<<<..<<<<<<<<...............>>>>>>
>><<<<<...<<<<...<<<<<<<<<<]]..>>>>>...>>>>>...>>>
>.>>>>>..<<<....<<<<<...........>>>>>....>>>.>>>>.
......>>>>>>.....<<<<<<<<<.......>>>>>....<<<.<<<<
<<<<....<<<<<..........>>>>>...>>>>.>>>>..>>>...>>
>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2otj The Structures of Antibiotics Bound to the E Site Region of the 50 S Ribosomal Subunit of Haloarcula marismortui: 13-Deoxytedanolide and Girodazole.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): T1 K3 K4 K5 R6 R8 G9 R11 T12 H13 G14 G15 G16 H18 K19 N20 R21 R22 G23 A24 G25 H26 R27 G28 G29 R30 G31 D32 G34 R35 K37 H38 E39 F40 H41 L46 G47 K48 G50 F51 K52 R53 P54 K56 V57 T63 D65 R67 D70 E71 K107 L109 G110 A111 G112 Q113 R115 S126
• Binding residue (residue number reindexed from 1): T1 K3 K4 K5 R6 R8 G9 R11 T12 H13 G14 G15 G16 H18 K19 N20 R21 R22 G23 A24 G25 H26 R27 G28 G29 R30 G31 D32 G34 R35 K37 H38 E39 F40 H41 L46 G47 K48 G50 F51 K52 R53 P54 K56 V57 T63 D65 R67 D70 E71 K102 L104 G105 A106 G107 Q108 R110 S121
• Binding affinity: PDBbind-CN: Kd=2nM

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:2otj, PDBe:2otj, PDBj:2otj
• PDBsum: 2otj
• PubMed: 17321546
• UniProt: P12737|RL15_HALMA Large ribosomal subunit protein uL15 (Gene Name=rpl15)