Structure of PDB 7ls1 Chain K3 Binding Site BS01

Receptor Information

>7ls1 Chain K3 (length=227) Species: 10090 (Mus musculus)

MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVV
RISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGC
IVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKE
DDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTK
KNKEEAAEYAKLLAKRMKEAKEKRQEQ

Ligand information

>7ls1 Chain m2 (length=1723)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugaccccccuucccggggggg
gaugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgca
cgccccccguggcggcgacgacccauucgaacgucugcccuaucaacuuu
cgaugguagucgccgugccuaccauggugaccacgggugacggggaauca
ggguucgauuccggagagggagccugagaaacggcuaccacauccaagga
aggcagcaggcgcgcaaauuacccacucccgacccggggagguagugacg
aaaaauaacaauacaggacucuuucgaggcccuguaauuggaaugagucc
acuuuaaauccuuuaacgaggauccauuggagggcaagucuggugccagc
agccgcgguaauuccagcuccaauagcguauauuaaaguugcugcaguua
aaaagcucguaguuggaucuugggagcgggcguccccgcccucucggcgg
ggcccgaagcguuuacuuugaaaaaauuagaguguucaaagcaggcccga
gccgccuggauaccgcagcuaggaauaauggaauaggaccgcgguucuau
uuuguugguuuucggaacugaggccaugauuaagagggacggccgggggc
auucguauugcgccgcuagaggugaaauucuuggaccggcgcaagacgga
ccagagcgaaagcauuugccaagaauguuuucauuaaucaagaacgaaag
ucggagguucgaagacgaucagauaccgucguaguuccgaccauaaacga
ugccgacuggcgaugcggcggcguuauucccaugacccgccgggcagcuu
ccgggaaaccaaagucuuuggguuccggggggaguaugguugcaaagcug
aaacuuaaaggaauugacggaagggcaccaccaggaguggagccugcggc
uuaauuugacucaacacgggaaaccucacccggcccggacacggacagga
uugacagauugauagcucuuucucgauuccgugggugguggugcauggcc
guucuuaguugguggagcgauuugucugguuaauuccgauaacgaacgag
acucuggcaugcuaacuaguuacgcgacccccgagcggucggcguccccc
aacuucuuagagggacaaguggcguucagccacccgagauugagcaauaa
caggucugugaugcccuuagauguccggggcugcacgcgcgcuacacuga
cuggcucagcgugugccuacccugcgccggcaggcgcggguaacccguug
aaccccauucgugauggggaucggggauugcaauuauuccccaugaacga
ggaauucccaguaagugcgggucauaagcuugcguugauuaagucccugc
ccuuuguacacaccgcccgucgcuacuaccgauuggaugguuuagugagg
cccucggaucggccccgccggggugcccuggcggagcgcugagaagacgg
ucgaacuugacuaucuagaggaaguaaaagucguaacaagguuuccguag
gugaaccugcggaaggaucauua

...<<<<<.[.((((>>>>><<<.<<<<<......<<..<......<<<.
<<<........<<....<<..........>>...>>.........<<...
.....<<<.<..........<<<...........<<.....<<.......
......>>......>>.........<<<<<..<<<<<<<....>>>>>>>
.>.>>>><<..<<<<<...<......>..>>>>>......>>....<<<.
<<<<......>>>>>>>.....>>>.......>.>>>.<<<....<<<..
.....<<<<.........>>>.>..>>>......>>>...<<<.<<<<..
..>>>>....>>>.>>.<<.<<<..........>>>.>>.<.<<<..>>>
.>...>>>>>>.........<<<....<<<.....>>>..>>>..>...>
>.......<<<<<<<<<.<<<....>>>..>>>>>.>>>>......<<..
<...........>..>>.........<<<<<.((...<.<<<<.....<<
..))>>.......>>>>>>>>>>...>>>>>.>>>.........<.<((.
....<.<<...<<<..........................<<.<......
......>>>....<<<<<.<.......<<...<.......>..<<.....
.....>>...>>......>.>>>..>>.........<.<..<<<<<<<..
.............>>>>>>>..>.>..........<<<<<.<.<...<<<
<..<<..<<<<<<<<...<<<......>>>......>>>>>>>>..>>..
.....<<....>>...>>>>..>..>>>.>>>...>>>...>>.>....<
<<<<<<...<...<<<<.........>>>>...>>>>>>>>.........
.<<<.<<.<<<..<.<<<<<<.<<<........>>>>>>>>>.>..>>>.
...<<.))>>...>>.....>>>.>.>.<<.......<<<<....>>>>.
....>>..)))).]<<<<<.<<<<<<<..<<..<<<<<..<<<.<<<<<<
......<<........>>..........<<<<<.<....<<<<<<.....
..<<.<<<........>>>.>>.....>>>>>>...<<.<<<..<<.<<<
<<<....<<<.<<<<......>>...<<<......>>>...>>.>>>...
.<<<...<...<<<...<<<<<<<<<<<<.......>>>>.>>>>.....
>>>>...>>>..>.....<<<<<.....>>>>>........>>>....>>
>.>>>.....>>>>>>>.....>.>>>>>...>>.>>>>.>>>.....<<
.<<<........<.......<<<<<<<<....>>>>>>>>....>.....
....>>>.>>............<<<<<..........>>>>>........
...>>>>>.....<<<<<<<<.......>>>>>>>>......>>...>>>
>>>>>>>.>>....<..<<.<..<<<<.<<....<<<<<<<<.<<<...<
<<.<<...<<<<<.<<<<<<<<<..>>>>>>>>>...>>>>>...>>.>>
>...>>>.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<
<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ls1 Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): K2 N4 S6 P8 G11 Q13 L15 S53 N56 Q59 G60 Q65 G66 L75 H81 C83 R85 R87 R88 T89 G90 R92 R94 K95 R132 L133 G134 K136 R137 S139 R140 K143 R154 R159 L162 K164 K167 R170 T171 K172 A173 P174 K175 I176 Q177 R178 R183 Q186 H187 K188 R189 R190 R191 I192 K195 R198 Q227
• Binding residue (residue number reindexed from 1): K2 N4 S6 P8 G11 Q13 L15 S53 N56 Q59 G60 Q65 G66 L75 H81 C83 R85 R87 R88 T89 G90 R92 R94 K95 R132 L133 G134 K136 R137 S139 R140 K143 R154 R159 L162 K164 K167 R170 T171 K172 A173 P174 K175 I176 Q177 R178 R183 Q186 H187 K188 R189 R190 R191 I192 K195 R198 Q227

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019901 protein kinase binding

Biological Process

• GO:0000082 G1/S transition of mitotic cell cycle
• GO:0000278 mitotic cell cycle
• GO:0001890 placenta development
• GO:0002181 cytoplasmic translation
• GO:0002309 T cell proliferation involved in immune response
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0006924 activation-induced cell death of T cells
• GO:0007369 gastrulation
• GO:0008284 positive regulation of cell population proliferation
• GO:0022605 mammalian oogenesis stage
• GO:0031929 TOR signaling
• GO:0033077 T cell differentiation in thymus
• GO:0042274 ribosomal small subunit biogenesis
• GO:0042593 glucose homeostasis
• GO:0043065 positive regulation of apoptotic process
• GO:0043066 negative regulation of apoptotic process
• GO:0048821 erythrocyte development

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030425 dendrite
• GO:0032040 small-subunit processome
• GO:0036464 cytoplasmic ribonucleoprotein granule
• GO:0044297 cell body
• GO:0048471 perinuclear region of cytoplasm
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ls1, PDBe:7ls1, PDBj:7ls1
• PDBsum: 7ls1
• PubMed: 34815424
• UniProt: P62754|RS6_MOUSE Small ribosomal subunit protein eS6 (Gene Name=Rps6)