Structure of PDB 7s0s Chain K Binding Site BS01

Receptor Information

>7s0s Chain K (length=133) Species: 1772 (Mycolicibacterium smegmatis)

LIKLQIQAGQANPAPPVGPALGQHGVNIMEFCKAYNAATESQRGNVIPVE
ITVYEDRSFTFALKTPPAAKLLLKAAGVQKGSGEPHKTKVAKVTWDQVRE
IAETKKADLNANDIDAAAKIIAGTARSMGITVE

Ligand information

>7s0s Chain C (length=3119)

aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugagacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaaccaggagguuggcuuagaagcagccacccuug
aaagagugcguaauagcucacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccaaaaccaccgugaccgcaccuuucgggguguggcguugg
uggggcugcaugggaccuucguugguaguagucaagcgauggggugacgc
aggaagguagccguaccggucagugguaauaccgggguaagccuguaggg
agucagauagguaaauccgucuggcauauauccugagaggugaugcauag
ccgagugaggcgaauucggugauccuaugcugccgagaaaagccucuagc
gaggacauacacggcccguaccccaaaccaacacagguggucagguagag
aauacuaaggcguacgagugaacuaugguuaaggaacucggcaaaaugcc
cccguaacuucgggagaagggggacccacauggcguguaagccuuuacgg
cccaagcgugaguggguggcacaaaccagugagaagcgacuguuuacuaa
aaacacagguccgugcgaagucgcaagacgauguauacggacugacgccu
gcccggugcuggaagguuaagaggacccguuaacucccuuugggggugaa
gcggagaauuuaagccccaguaaacggcggugguaacuauaaccauccua
agguagcgaaauuccuugucggguaaguuccgaccugcacgaauggcgua
acgacuucucaacugucucaaccauagacucggcgaaauugcacuacgag
uaaagaugcucguuacgcgcggcaggacgaaaagaccccgggaccuucac
uacaacuugguauuggugcucgauacgguuuguguaggauaggugggaga
cugugaagcucacacgccaguguggguggagucguuguugaaauaccacu
cugaucguauugggccucuaaccucggaccguauauccgguucagggaca
gugccugguggguaguuuaacuggggcgguugccuccuaaaauguaacgg
aggcgcccaaagguucccucaaccuggacggcaaucagguguugagugua
agugcacaagggagcuugacugcgagacggacaugucgagcagggacgaa
agucgggacuagugauccggcaccucugaguggaaggggugucgcucaac
ggauaaaagguaccccggggauaacaggcugaucuuccccaagaguccau
aucgacgggaugguuuggcaccucgaugucggcucgucgcauccuggggc
uggagcaggucccaaggguugggcuguucgcccauuaaagcggcacgcga
gcuggguuuagaacgucgugagacaguucggucucuauccgccgcgcgcg
ucagaagcuugaggaaaccugucccuaguacgagaggaccgggacggacg
aaccucugguauaccaguugucccaccaggggcacggcuggauagccacg
uucggacaggauaaccgcugaaagcaucuaagcgggaaaccucuuccaag
accaggcuucucacccucuaggagggauaaggccccccgcagaccacggg
auugauagaccagaccuggaagccuaguaauaggugcagggaacuggcac
uaaccggccgaaaacuuac

<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.[[.(((.>>>.....<<<<<<<..<<<)))..]].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<..........(((((....
..>>>>>>>>>......<<<<..<.....<<<<<<<))))).>>>>>>><
<<<<<.......<<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>.....>.
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<....>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<<<<.<...<<<<<
....>>>>>.....>>>>>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
<<<<<<<<<.........<<<<.<<<..<<<.........>>>..>>>..
.......<<......>>...>>>>.....>>>>>>>>>.>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<
<<<........>>>>>))<<<<<<<<<<.......<<<<..<<<<<<<<.
.....<<<<.<<...<<<<<....<<<<<<<<....>>>>>>>>....>>
>>>...>>.>>>>.....>>>>>>>>....>>>>.<<...<<<<<...<<
<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<<...<<<
<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...>>...<
<<......>>>.....>>>>...>>>...>>>>........>>>>>..>>
....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<...
..>>>>..>>>>..<<<<<...<<<<<<<<.<<...............<<
<<<...............>>>>><<<<<<...<<<<....<<<.....>>
>....>>>>..>>>>>>............<<<<<<<<<..<<<.......
..>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.
<<<..<<<<<<<..<<<<.<<<....<<<<<.<<<<<<...>>>>>>..>
>>>>....>>>.>>>>>>>>>..>>>>><<<<<.........>>>>>...
.....<...........<<<<<<.....>>>>>>.....>....>>>>>.
.>>.>>>>>>>.....>>.>>>>>>>>>>>>>>>.....>.((..<<<<<
......))>>>>>..<<<<<<<<.<<<.<<......<<<<<<<<<<..<<
<<<.<<<.<<<<<.<<.<<<<<<<<<<<..............<<<<..<<
.......<<<<<<<......>>>>>>>....>>............>>>>.
....>>>>>>>>>>>.>>...<<<.<<<<<<......>>>>>>>>>....
>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<...........>>
>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.
(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<...
.>>>.>>)))]......<<<<<<<.<<..>>>>..>>>>>>>>>......
.......>>>>..>>>>>>.......<<<<.<.<.<<<<...<<......
.>>...>>>>.>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<
<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...
>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>>>>>..
..<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<
<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>>>>.>
>>>>...<<<....<<<<<...........>>>>>....>>>.>>>>...
....>>>>>>>..<<<<<...>>>>>....<<<<.<<<<.......>>>>
.....<<<.<<<<.<<<....<<<<<....>>>>>...>>>..>>>>..>
>>...>>>>....>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7s0s 50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA.
• Resolution: 3.05 Å
• Binding residue (original residue number in PDB): K12 Q14 P28 Q32 K73 T74 P75 G92 E93 K128 I129 T133 R135 S136 M137
• Binding residue (residue number reindexed from 1): K3 Q5 P19 Q23 K64 T65 P66 G83 E84 K119 I120 T124 R126 S127 M128

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7s0s, PDBe:7s0s, PDBj:7s0s
• PDBsum: 7s0s
• PubMed: 35357969
• UniProt: A0QS45|RL11_MYCS2 Large ribosomal subunit protein uL11 (Gene Name=rplK)