Structure of PDB 7asm Chain K Binding Site BS01

Receptor Information

>7asm Chain K (length=137) Species: 1280 (Staphylococcus aureus)

MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITSRQIESA
RIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPG
RILFEVAGVSEEVAREALRLASHKLPVKTKFVKREEL

Ligand information

>7asm Chain A (length=2834)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuacggaguuacaaaggacgacauuagacgaaucaucuggaaa
gaugaaucaaagaagguaauaauccuguagucgaaaauguugucucucuu
gaguggauccugaguacgacggagcacgugaaauuccgucggaaucuggg
aggaccaucuccuaaggcuaaauacucucuagugaccgauagugaaccag
uaccgugagggaaaggugaaaagcaccccggaaggggagugaaauagaac
cugaaaccgugugcuuacaaguagucagagcccguuaaugggugauggcg
ugccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagc
aguaaauguggagccguagcgaaagcgagucugaauagggcguuuaguau
uuggucguagacccgaaaccaggugaucuacccuuggucagguugaaguu
caggcugaauggaggaccgaaccgacuuacguugaaaagugagcggauga
acugaggguagcggagaaauuccaaucgaaccuggagauagcugguucuc
uccgaaauagcuuuagggcuagccucaagugaugauuauuggagguagag
cacuguuuggacgaggggccgguuaccgaauucagacaaacuccgaaugc
caauuaauuuaacuugggagucagaacaugggugauaagguccguguucg
aaagggaaacagcccagaccaccagcuaaggucccaaaauauauguuaag
uggaaaaggauguggcguugcccagacaacgucgagugacacugcgccga
aaauguaccggggcuaaacauauuaccgaagcuguggauuguccuuugga
caaugguaggagagcguucuaagggcguugaagcaugaucguaaggacau
guggagcgcuuagaagugagaaugccggugugaguagcgaaagacgggug
agaaucccguccaccgauugacuaagguuuccagaggaaggcucguccgc
ucuggguuagucggguccuaagcugaggccgacaggcguaggcgauggau
aacagguugauauuccuguaccaccuauaaucguuuuaaucgaugggggg
acgcaguaggauaggcgaagcgugcgauuggauugcacgucuaagcagua
aggcugaguauugguacucguuaaggcugagcugugauggggagaagaca
uugugucuucgagucguugauuucacacugccgagaaaagccucuagaua
gaaaauaggugcccguaccgcaaaccgacacagguagucaagaugagaau
ucuaaggugagcgagcgaacucucguuaaggaacucggcaaaaugacccc
guaacuucgggagaaggggugcucuuuaggguuaacgcccagaagagccg
cagugaauaggcccaagcgacuguuuaucaaaaacacaggucucugcuaa
accguaaggugauguauaggggcugacgccugcccggugcuggaagguua
agaggagugguuagcuucugcgaagcuacgaaucgaagccccaguaaacg
gcggccguauaacgguccuaagguagcgaaauuccuugucggguaaguuc
cgacccgcacgaaaggcguaacgauuugggcacugucucaacgagagacu
cggugaaaucauaguaccugugaagaugcagguuacccgcgacaggacgg
aaagaccccguggagcuuuacuguagccugauauugaaauucggcacgcu
uguacaggauagguaggagccuuugaaacgugagcgcuagcuuacgugga
ggcgcuggugggauacuacccagcuguguuggcuuucuaacccgcaccac
uuaucguggugggagacagugucaggcgggcaguuugacuggggcggucg
ccuccuaaaagguaacggaggcgcucaaagguucccucagaaugguugga
aaucauucauagaguguaaaggcauaagggagcuugacugcgagaccuac
aagucgagcagggucgaaagacggacuuagugauccggugguuccgcaug
gaagggccaucgcucaacggauaaaagcuaccccggggauaacaggcuua
ucucccccaagaguucacaucgacggggagguuuggcaccucgaugucgg
cucaucgcauccuggggcuguagucggucccaaggguugggcuguucgcc
cauuaaagcgguacgcgagcuggguucagaacgucgugagacaguucggu
cccuauccgucgugggcguaggaaauuugagaggagcuguccuuaguacg
agaggaccgggauggacauaccucugguguaccaguugucgugccaacgg
cauagcuggguagcuauguguggacgggauaagugcugaaagcaucuaag
caugaagccccccucaagaugagauuucccaacuucgguuauaagauccc
ucaaagaugaugagguuaauagguucgagguggaagcauggugacaugug
gagcugacgaauacuaaucgaucgaagacuuaau

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[.<.....
.<<<<<<.[...((.>>>.....<<<<<<<..<<<))...].>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>.....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<...<<<<.......<<<<<<<<<<.<<<...<<<<<......
>>>>>((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>.
>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<<<
<<..<<<<>>>>>..)))......]].>>>>>>>.]].............
..<<....>>....]].........<<<<....>>>>.............
.....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>.
..<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<
<.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>>>
>.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<
<<<.>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....
>>>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<
<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<
...<<<<<<<<...<<.........>>...>>>>>>>>..>>.>.....>
>>>>.>>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>.
...<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<....
.......<<..<<<<.<<<<........<..>....>>>>.>>>>.>>..
.........))).....>>>>>>.>>>...>>>>>>><<<<<<<...>>>
>>>>....>>>>>><.<<<<<<<.<<......<<<<<.<<....>>.>>>
>>...>>.>>>>>>>..>.....>>>>>><......<<....<<<<<<..
.....>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>
>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>..
.<<<<<........>>>>>))<<<<<<<..........<<<...<<<<..
..<<<<...<<...<....<.<<<...{.{...>>>.>.<....<<<<..
.<<<.<<<<<<..>>>>>>......>>>...>>>>.....>.........
.................>....>>...>>>>.........>>>>..>>>.
....>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<.......
>>>..>>>>..<<<<....<<<<<<<<.<<.........<.....<<<<<
....<<..>>.....>>>>><<<<<<..<.........>...>>>>>>..
..........<<<<<<<<<..<<<.........>>>..<<<<<<<.....
<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<..
.<<...<<<<<<...........>>>>>>...>>...>>>>>>>>..>>>
>><<<<<.....>>>>>........<...........<<<<<<.....>>
>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>
>>>>.....>.....<<<<<........>>>>>..<<<<<<<<.(((.[[
......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<<.<<<<<<<<<.
.............<<<..<<.........<<<<<<....>>>>>>.....
.>>............>>>....>.>>>>>>>>.>>>>....<<.<<<<<<
.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><
<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<.....
..>>>>>>.....<<.[.(((>>....>>>>>>>...<<<<..<<<....
..>>>..>>>>.<<<....>>>.>>)))]......<<<<<<<.((..>>>
>..))>>>>>>>.............>>>>..]]>>>>.......<<<<.<
.<<<<<<...<<.......>>...>>>>>>...>>>>.>.......<<<<
<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>>
>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>...
.]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<.......
........>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.}.}>>>>
>>..>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>
>>>....>>>.>>>>.......>>>>>>>.<<<....>>>....<<<<<<
<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>.
..>>>..>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7asm Staphylococcus aureus 50S after 30 minutes incubation a 37C
• Resolution: 2.48 Å
• Binding residue (original residue number in PDB): K5 R6 Y9 R11 H13 R14 K22 G24 W41 R45 Q46 S49 R56 F69 H71 E80 V81 R82 M83 G84 G86 K87 H123 K124 P126 K128
• Binding residue (residue number reindexed from 1): K5 R6 Y9 R11 H13 R14 K22 G24 W41 R45 Q46 S49 R56 F69 H71 E80 V81 R82 M83 G84 G86 K87 H123 K124 P126 K128

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7asm, PDBe:7asm, PDBj:7asm
• PDBsum: 7asm
• UniProt: Q2FW13|RL16_STAA8 Large ribosomal subunit protein uL16 (Gene Name=rplP)