Structure of PDB 7afi Chain K Binding Site BS01

Receptor Information

>7afi Chain K (length=116) Species: 562 (Escherichia coli)

KQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAA
QVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDV
TPIPHNGCRPPKKRRV

Ligand information

>7afi Chain A (length=1066)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacg
aguggcggacgggugaguaaugucugggaaacugccugauggagggggau
aacuacuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagag
ggggaccuucgggccucuugccaucggaugugcccagaugggauuagcua
guaggugggguaacggcucaccuaggcgacgaucccuagcuggucugaga
ggaugaccagccacacuggaacugagacacgguccagacuccuacgggag
gcagcaguggggaauauugcacaaugggcgcaagccugaugcagccaugc
cgcguguaugaagaaggccuucggguuguaaaguacuuucagcggggagg
aagggaguaaaguuaauaccuuugcucauugacguuacccgcagaagaag
caccggcuaacuccgugccagcagccgcgguaauacggagggugcaagcg
uuaaucggaauuacugggcguaaagcgcacgcaggcgguuuguuaaguca
gaugugaaauccccgggcucaaccugggaacugcaucugauacuggcaag
cuugagucucguagagggggguagaauuccagguguagcggugaaaugcg
uagagaucuggaggaauaccgguggcgaaggcggcccccuggacgaagac
ugacgcucaggugcgaaagcguggggagcaaacaggauuagauacccugg
uaguccacgccguaaacgaugucgacuuggagguugugcccuugaggcgu
ggcuuccggagcuaacgcguuaagucgaccgccuggggaguacggccgca
agguuaaaacucaaaugaauugacgggggcgccuugaacacacccaccau
gggaguggguugcaaaagaaguagguagcuuaaccuucgggagggcgcuu
accacuuugugauucaugacuggggugaagguaaccguaggggaaccugc
gguuggaucaccuccu

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<....
.<<<.<<<..<<<..<<.<...<<<<<<.<<<....>>>.>>>.>>>.>.
.>>>>>......<<.......<<<<<<<..<<...<<<<<<<.<.<<...
..<<<<........>>>>......>>.>.....<<<....>>>....<<<
<<...<<....>>.>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<.
.<<<<<<<.........>>>>>>>>>>......>>>..<<<<<<<<....
>>>...>>>>>.>>.<<<<<.<.........>>>>>>.<<<......>>>
...>>>>>>.........<<<...<<<<<....>>>>.>>>>..>>.>>>
>>>..<<<<......<<<<....>>>>.....>>>>...<.<<<<<....
..<.<<<<<<<<.......>>>>>>>>.>........>>>>>....>..<
<<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>
>>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<<<
<<<<<......<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>
>...<<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>>.
.....>>>>>>>>...........<<....>>.>>>>>>>..>>>>.>>>
>...>>>...>>>>....<<<<<<...<<...<<<<.........>>>>.
..>>>>>>>>..........<<<<<<.<<<<<<<.<<<<<.....>>>>>
.>>>>>>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<...
.>>>>....>>>..)))).]<<<....<<..>>......<<.....<<..
<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.>>>>>>>>
>>...>>>>>>>>>>>>..>>..>>.....>><<<<<<<<<....>>>>>
>>>>...>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7afi A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.
• Resolution: 3.53 Å
• Binding residue (original residue number in PDB): H24 N28 N29 T33 Q38 G39 N40 A41 W44 T46 G48 R53 G54 P117 H118 N119 G120 C121 R122 P124 K125 R127 R128 V129
• Binding residue (residue number reindexed from 1): H11 N15 N16 T20 Q25 G26 N27 A28 W31 T33 G35 R40 G41 P104 H105 N106 G107 C108 R109 P111 K112 R114 R115 V116

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7afi, PDBe:7afi, PDBj:7afi
• PDBsum: 7afi
• PubMed: 34088665
• UniProt: P0A7R9|RS11_ECOLI Small ribosomal subunit protein uS11 (Gene Name=rpsK)