Structure of PDB 6o8y Chain K Binding Site BS01

Receptor Information

>6o8y Chain K (length=145) Species: 1351 (Enterococcus faecalis)

MRTTYMAKAGEVERKWYVVDATDVPLGRLSAVVASVLRGKNKPTFTPHVD
TGDFVIVINADKVKLTGKKATDKIYYRHSMYPGGLKSVTAGELRDKNSRR
LIETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITN

Ligand information

>6o8y Chain A (length=2897)

uuaagugaauaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacuaacaccgauaugcuuuggggagcuguaaguaagcuauga
uccagagauuuccgaaugggggaacccaauaucuuuuauaggauauuacu
uuucagugaauacauagcugauuagagguagacgcagagaacugaaacau
cuuaguaccugcaggaagagaaagaaaauucgauucccugaguagcggcg
agcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguuguag
gacuccgauaugguagucuguuaguauaguugaaggauuuggaaaauucc
gcuaaagagggugaaagccccguagacgaaaugcugacaacaccuaggag
gauccugaguacggcggaacacgagaaauuccgucggaauccgcggggac
caucccgcaaggcuaaauacucccuagugaccgauagugaaccaguaccg
ugagggaaaggugaaaagcaccccggaaggggagugaaauagauccugaa
accgugugccuacaacaagucaaagcucguuaaugagugauggcgugccu
uuuguagaaugaaccggcgaguuacgauugcaugcgagguuaagucgaag
agacggagccgcagcgaaagcgagucugaauagggcgaaugaguauguag
ucguagacccgaaaccaugugaucuacccauguccagguugaaggugcgg
uaaaacgcacuggaggaccgaacccacguacguugaaaagugcggggaug
agguguggguagcggagaaauuccaaacgaacuuggagauagcugguucu
cuccgaaauagcuuuagggcuagccucggaauugagaaugauggagguag
agcacuguuuggacuaggggcccaucggguuaccgaauucagauaaacuc
cgaaugccauucauuuauauccgggagucagacugcgagugauaagaucc
guagucgaaagggaaacagcccagaccaccagcuaaggucccaaaauaua
uguuaaguggaaaaggaugugggguugcacagacaacuaggauguuggcu
uagaagcagccaccauuuaaagagugcguaauagcucacuagucgaguga
cccugcgccgaaaauguaccggggcuaaacauauuaccgaagcuguggac
uacaccauuagguguagugguaggagagcguucuaagggcguugaagguc
gaucgugaggacggcuggagcgcuuagaagugagaaugccgguaugagua
gcgaaagacaggugagaauccuguccaccguaugacuaagguuuccuggg
gaaggcucguccgcccaggguuagucgggaccuaagccgaggccgauagg
cguaggcgauggacaacagguugauauuccuguaccaguuguuuuuguuu
gagcaauggagggacgcaguaggcuaaggaaugcaugcgauuggaagugc
auguccaagcaaugagucuugaguagaguuaaaugcuuuacucuuuaagg
acaaguugugacggggagcgaaauaguagcgaaguuccugacgucacacu
gccaagaaaagcuucuagugagaaaacaacugcccguaccguaaaccgac
acagguagucgaggagaguauccuaaggugagcgagcgaacucucguuaa
ggaacucggcaaaaugaccccguaacuucgggagaaggggugcugacuuc
ggucagccgcagugaauaggcccaagcgacuguuuaucaaaaacacaggu
cucugcaaaaucguaagaugaaguauaggggcugacgccugcccggugcu
ggaagguuaagaggauggguuagcuucggcgaagcucagaauugaagccc
caguaaacggcggccguaacgguccuaagguagcgaaauuccuugucggg
uaaguuccgacccgcacgaaaggcguaacgauuugggcacugucucaacg
agagacucggugaaauuuuaguaccugugaagaugcagguuacccgcgac
aggacggaaagaccccauggagcuuuacuguaguuugauauugaguguuu
guaccacauguacaggauagguaggagccgaugagaccggaacgcuaguu
ucggaggaggcgcuggugggauacuacccuuguguuaugaacccucuaac
ccgcaccacuaaucguggugggagacagugucagaugggcaguuugacug
gggcggucgccuccuaaaagguaacggaggcgcccaaagguucccucaga
augguuggaaaucauucgaagaguguaaaggcagaagggagcuugacugc
gagaccuacaagucgagcagggacgaaagucgggcuuagugauccggugg
uuccgcauggaagggccaucgcucaacggauaaaagcuacccuggggaua
acaggcuuaucucccccaagaguccacaucgacggggagguuuggcaccu
cgaugucggcucgucgcauccuggggcuguagucggucccaaggguuggg
cuguucgcccauuaaagcggcacgcgagcuggguucagaacgucgugaga
caguucggucccuauccgucgcgggcguuggaaauuugagaggagcuguc
cuuaguacgagaggaccgggauggacuuaccgcugguguaccaguuguuc
ugccaagggcauugcuggguagcuauguagggaagggauaaacgcugaaa
gcaucuaagugugaagcccaccucaagaugagauuucccauuucuuuaag
aaaguaagaccccugagagaugaucagguagauagguuggaaguggaagg
cuagugauaguuggagcggaccaauacuaaucggucgaggacuuaac

<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
..<<<<<.........>>>>>..>>.>>....<<<<<......<......
>.......>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<......>>>>>>>......<<
<<<<<<.....<<<.<<.<<<<<<<<<.<<<...<<<.<......>.>>>
((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>..>>>>
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..)))......]].>>>>>>>.]]...............<<.
...>>....]].........<<<<....>>>>.............}....
>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<.<
........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<<.....
..>>...>>>...<<....>>...<<<.....>>>.......>>>>>>>>
>>>>>..<<.<...<<<.<<.....<<<<<<<<<..<<<....<<<<<<.
.....>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>...
.>>>>>>>>>...<<<.....>>>......>>.>>>......>.>>..<<
<<<<....<<<<<....>>>>><<<<<<<<..<..<<<<<<<<<......
.....<<<<<<<<.......<<<...>>>........>>>>>>>>.....
......>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<......>>.>
>>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....<<<
<<<...........<<..<<<<<<<<<........<<.....<...<...
..........>...>..........................>>....>>>
>>>>>>.>>...........))).....>>>>>>.>>>...>>>>>>><<
<<<<<<....>>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<
<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......
<<....<<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<<<<
...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>
>...>>>...>>...<<<<<........>>>>>))<<<<<<<........
..<<...<<<<....<<<<...<...<<<<<.........[.[.......
....<...<<<<<..<<<<.<<<<<<<<<.....>>>>>>>>>.....>>
>>..>>>>>....>...<<.........>>....>>>>>....>....>>
>>.........>>>>..>>......>>>>>>>>>>>.>>>.....<<...
...>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<
<.........<.....<<<<<....<<..>>.....>>>>><<<<<<...
.>>>>>>............<<<<<<<<<..<<<.........>>>..<<<
<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<
<<..<<<...<<...<<<<<..<<....>>...>>>>>...>>...>>>>
>>>>..>>>>><<<<<...>>>>>........<...........<<<<<<
.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.(((.((......<<<<.((<<<<..<<<<<.<<<<<<<<<.<<<.<<<<
<<<.<<<<...........<<<<<..<<<.......<<<<<<<....>>>
>>>>....>>>............>>>>>..>>>>.>>>>>>>.>>>....
<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<.
....>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<
<<<<.......>>>>>>.....<<.((.((>>....>>>>>>>...<<<<
..<<<......>>>..>>>>.<<<....>>>.>>))))......<<<<<<
<.((..>>>>..))>>>>>>>.............>>>>..))>>>>....
...<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>..
.....<.<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<
<.....>>>>>.....>>>>>..>...>>>..>....<<<<<<<....>>
>.>>>>....))..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<
<<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<
<.].]>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<....
.......>>>>>....>>>.>>>>.......>>>>>>>..<<<......>
>>.....<<<<<<<<<.......>>>>>....<<<<<<<<..<<....<<
<<<....>>>>>...>>...>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6o8y Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
• Resolution: 4.14 Å
• Binding residue (original residue number in PDB): R2 M6 K8 P25 R28 R38 K40 P47 H48 T66 K69 K73 Y76 R77 H78 S79 Y81 K86 G108 M109 P111 K112 N113 T114 L115 R117 P132 H133 A135 Q136
• Binding residue (residue number reindexed from 1): R2 M6 K8 P25 R28 R38 K40 P47 H48 T66 K69 K73 Y76 R77 H78 S79 Y81 K86 G108 M109 P111 K112 N113 T114 L115 R117 P132 H133 A135 Q136

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6o8y, PDBe:6o8y, PDBj:6o8y
• PDBsum: 6o8y
• PubMed: 33004869
• UniProt: A0A1B4XSB2