Structure of PDB 4wfa Chain K Binding Site BS01

Receptor Information

>4wfa Chain K (length=119) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

RKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVVEKLITLGKK
GDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTRI
LKQGPRRGDGAESVIIELV

Ligand information

>4wfa Chain X (length=2711)

gauuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccaau
gaaggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuu
gauccagagauuuccgaauggggaaacccagcaugaguuaugucauguua
ucgauauguauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguugag
gacacucuaugaguuacaaaggacgacauuagacgaaucaucuggaaaga
ugaaucaaagaagguaauaauccuguagucgaaaauguugucucucuuga
guggauccugaguacgacggagcacgugaaauuccgucggaaucugggag
gaccaucuccuaaggcuaaauacucucuagugaccgauagugaaccagua
ccgugagggaaaggugaaaagcaccccggaaggggagugaaauagaaccu
gaaaccgugugcuuacaaguagucagagcccguuaaugggugauggcgug
ccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagcag
uaaauguggagccguagcgaaagcgagucugaauagggcguuuaguauuu
ggucguagacccgaaaccaggugaucuacccuuggucagguugaaguuca
ggcugaauggaggaccgaaccgacuuacguugaaaagugagcggaugaac
ugaggguagcggagaaauuccaaucgaaccuggagauagcugguucucuc
cgaaauagcuuuagggcuagccucaagugaugauuauuggagguagagca
cuguuuggacgaggggguuaccgaauucagacaaacuccgaaugccaauu
aauuuaacuugggagucagaacaugggugauaagguccguguucgaaagg
gaaacagcccagaccaccagcuaaggucccaaaauauauguuaaguggaa
aaggauguggcguugcccagacaacuagcucacuagucgagugacacugc
gccgaaaauguaccggggcuaaacauauuaccgaagcuguggauuguccu
uggacaaugguaggagagcguucuaagggcguugaagcaugaucguaagg
acauguggagcgcuuagaagugagaaugccggugugaguagcgaaagacg
ggugagaaucccguccaccgauugacuaagguuuccagaggaaggcucgu
ccgcucuggguuagucggguccuaagcugaggccgacaggcguaggcgau
ggauaacagguugauauuccuguaccaccuauaaucguaucgaugggggg
acgcaguaggauaggcgagcgugcgauuggaugcacgucuaagcaguagg
cugaguaaaauccgguacucggcugagcugugauggggagaagagagucg
uugauuucacacugccgagaaaagccucuagauagaauaggugcccguac
cgcaaaccgacacagguagucaagaugagaauucuaaggugagcgagcga
acucucguuaaggaacucggcaaaaugaccccguaacuucgggagaaggg
gugcucuuuaggguuaacgcccagaagagccgcagugaauaggcccaagc
gacuguuuaucaaaaacacaggucucugcuaaaccguaaggugauguaua
ggggcugacgccugcccgugcuggagguuaagaggagugguuagcugcga
agcuacgaaucgaagccccaguaaacggcggccuaagguagcgaaauucc
uugucggguaaguuccgacccgcacgaaaggcguaacgauuugggcacug
ucucaacgagagacucggugaaaucauaguaccugugaagaugcagguua
cccgcgacaggacggaaagaccccguggagcuuuacuguagccugauauu
gaaauucuggcuuucuaacccgcaccacuuaucguggugggagacagugu
caagcgggcaguuugacuggggcggucgccuccuaaaagguaacggaggc
gcucaaagguucccucagaaugguuggaaaucauucauagaguguaaagg
cauaagggagcuugacugcgagaccuacaagucgagcagggucgaaagac
ggacuuagugauccggugguuccgcauggaagggccaucgcucaacggau
aaaagcuaccccggggauaacaggcuuaucucccccaagaguucacaucg
acggggagguuuggcaccucgaugucggcucaucgcauccuggggcugua
gucggucccaaggguugggcuguucgcccauuaaagcgguacgcgagcug
gguucagaacgucgugagacaguucggucccuauccgucgugggcguagg
aaauuugagaggagcuguccuuaguacgagaggaccgggauggacauacc
ucugguguaccaguugucgugccaacggcauagcuggguagcuaugugug
gacgggauaagugcugaaagcaucuaagcaugaagccccccucaagauga
gauuucccaacuugguuauaagaucccucaaagaugaugagguuaauagg
uucgagguggaagcauggugacauguggagcugacgaauacuaaucgauc
gaagacuuaau

..<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.
<<<<<<<<<.......>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>.....<<
<<<<<<<...<..........<<<<<<<<<.<<<...<<<<<......>>
>>>((......<<<.....))>>>...>>>...>>>>>>>>.>...>.>>
>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<
..<<<<>>>>>..)))......]].>>>>>>>.]]...............
<<....>>....]].........<<<<....>>>>...............
...>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...
<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<..
.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>.
>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<.
....>>>>.....>>>...<<<.<<<<<........>>>>>>>>....>>
>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<<
<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<..
.<<<<<<<<...<<......>>...>>>>>>>>..>>.>.....>>>>.>
>>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>....<<
<......>>>...<<<<.<<<...<<<(((.....<<<<<<.........
..<<..<<<<.<<<<........<<<<......>>>>....>>>>.>>>>
.>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<<.
.>>>>>>>....>>>>>>..<<<<<<<.<<......<<<<<.<<....>>
.>>>>>...>>.>>>>>>>........>>>>>><......<<....<<<<
<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....
>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>...
>>...<<<<<........>>>>>))<<<<<<<......<<<...<<<<..
..<<<<...<<...<...<.<<<...{.{..>>>.>.<....<<<<<.<<
<.<<.<..........>.>>.>>>..>>>>>.....>.............
.>....>>...>>>>.........>>>.>.>>>...>>>>>>>>>>>..>
>.....<<......>>>>>>>.<<<<....>.>>>..>>>>..<<<<...
.<<<<<<<<.<<...............<<<<<.....<..>......>>>
>><<<<<<<.<<<<....>>>>.>>>>>>>............<<<<<<<<
<..<<<.........>>>..<<<<<<<.....<<<....>>>.......>
>>>>>>.<<<<<..<<.<<<<<<<.<<<...<<...<<<<<..<<.>>..
.>>>>>...>>...>>>>>>>>..>>>>............<.........
..<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>....
.>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..
<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<..<<.<<<<<<.
<<<<.......>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>
>>.>>...>>>>..<<<<.....>>>><<<<<<...........>>>>>>
..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>
>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>
.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>..........
...>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>.
..>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<...
...>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.
>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<
<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<.
..<<<<...<<<<<<<<<<<.}.}>>>>>>..>>>>>...>>>>.>>>>>
...<<<....<<<<<...........>>>>>....>>>.>>>>.......
>>>>>>>.<<<<.>>>>....<<<<<<<<<.......>>>>>....<<<<
<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>
....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wfa Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.
• Resolution: 3.392 Å
• Binding residue (original residue number in PDB): R4 K5 L6 R8 S10 R13 A15 R18 I30 E31 T32 T33 A35 K38 E39 R41 E45 K46 T49 R59 R60 K64 R67 N68 Q97 G98 G99 T101 R102 K105 R109 R110 G111 D112 A114
• Binding residue (residue number reindexed from 1): R1 K2 L3 R5 S7 R10 A12 R15 I27 E28 T29 T30 A32 K35 E36 R38 E42 K43 T46 R56 R57 K61 R64 N65 Q94 G95 G96 T98 R99 K102 R106 R107 G108 D109 A111

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wfa, PDBe:4wfa, PDBj:4wfa
• PDBsum: 4wfa
• PubMed: 26464510
• UniProt: Q2FW33|RL17_STAA8 Large ribosomal subunit protein bL17 (Gene Name=rplQ)