Structure of PDB 8phj Chain J Binding Site BS01

Receptor Information
>8phj Chain J (length=98) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
RIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLIS
PHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
Ligand information
>8phj Chain A (length=1503) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacaggaaguuugcugacgaguggcggacgg
gugaguaaugucugggaaacugccugauggagggggauaacuacuggaaa
cgguagcuaauaccgcauaacgucgcaagaccaaagagggggccucuugc
caucggaugugcccagaugggauuagcuaguaggugggguaacggcucac
cuaggcgacgaucccuagcuggucugagaggaugaccagccacacuggaa
cugagacacgguccagacuccuacgggaggcagcaguggggaauauugca
caaugggcgcaagccugaugcagccaugccgcguguaugaagaaggccuu
cggguuguaaaguacuuucagcggggaggaagggaguaaaguuaauaccu
uugcucauugacguuacccgcagaagaagcaccggcuaacuccgugccag
cagccgcgguaauacggagggugcaagcguuaaucggaauuacugggcgu
aaagcgcacgcaggcgguuuguuaagucagaugugaaauccccgggcuca
accugggaacugcaucugauacuggcaagcuugagucucguagagggggg
uagaauuccagguguagcggugaaaugcguagagaucuggaggaauaccg
guggcgaaggcggcccccuggacgaagacugacgcucaggugcgaaagcg
uggggagcaaacaggauuagauacccugguaguccacgccguaaacgaug
ucgacuuggagguugugccggcguggcuuccggagcuaacgcguuaaguc
gaccgccuggggaguacggccgcaagguuaaaacucaaaugaauugacgg
gggcccgcacaagcgguggagcaugugguuuaauucgaugcaacgcgaag
aaccuuaccuggucuugacauccacggaaguuuucagagaugagaaugug
ccgaaccgugagacaggugcugcauggcugucgucagcucguguugugaa
auguuggguuaagucccgcaacgagcgcaacccuuauccuuuguugccag
cgguccggccgggaacucaaaggagacugccagugauaaacuggaggaag
guggggaugacgucaagucaucauggcccuuacgaccagggcuacacacg
ugcuacaauggcgcauacaaagagaagcgaccucgcgagagcaagcggac
cucauaaagugcgucguaguccggauuggagucugcaacucgacuccaug
aagucggaaucgcuaguaaucguggaucagaaugccacggugaauacguu
cccgggccuuguacacaccgcccgucacaccaugggaguggguugcaaaa
gaaguagguagcuuaaccuucgggagggcgcuuaccacuuugugauucau
gacuggggugaagucguaacaagguaaccguaggggaaccugcgguugga
uca
.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<...<<<<<<.>>>.>>>.>..>>>>>......
<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<<.....
.>>>>>......>>.>.....<<<....>>>....<<<<<<.>>>>>>.>
>>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<.........>>>>
>>>>>>......>>>..<<<<<<<<....>>>>...>>>>.>>.<<<<<.
<.........>>>>>>.<<<<....>>>>...>>>>>>.........<<<
....<<<<....>>>>..>>>..>>.>>>>>>..<<<<......<<<<<.
.>>>>>.....>>>>...<.<<<<<......<.<<<<<<<<.......>>
>>>>>>.>........>>>>>....>..<<<<<(((...<<<<<.....<
<.)))>>.......>>>>>>>>>>..>>>>>>>>>..........<<<((
.....<<<<...<<<.<<<<<<<.<<<<<<<<<<......<<<<<<....
.>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<
<....<<<<<<<<...<<<......>>>......>>>>>>>>........
...<<....>>.>>>>>>>..>>>>.>>>>...>>>...>>>>....<<<
<<<...<<...<<<<.<.....>.>>>>...>>>>>>>>..........<
<<<<<.<<<<<<<<<<<<..>>>>>>>>>>>>..<<..))>>.....>>>
>>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<<.
<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>...
.......<<<<<<<......<<<<<<<..<<<<<<<....>>>>>>>...
.....>>>>>.>>.<<<.<<<..<<<<<<.......<<<<<<<<<....>
>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<<...<<..
<<<.....>>>>>....>>>>>>>.....<<<<<.....>>>>>......
..>>>>.........>>>...>>>>>>>>>...>>>>>>>...>>.>>>>
>>>>.....<<<<<<<.....<<<..<<...<<<....>>>...>>....
>>>.....>>>>>>>......<....<<<<<<<........>>>>>>>..
..>.....>>>>>>....<<<<<<<.........>>>>>>>......>>.
..>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<<<<<<<<.
...<<<<<<.<<<<..<<....>>.>>>>>>>>>>...>>>>>>>>>>>>
..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>...
...
Receptor-Ligand Complex Structure
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PDB8phj Ribosomal stalk-captured CARF-RelE ribonuclease inhibits translation following CRISPR signaling
Resolution3.67 Å
Binding residue
(original residue number in PDB)
R7 R9 K11 H15 R37 G38 P39 I40 P41 L42 P43 T44 R45 I53 S54 P55 H56 V57 N58 K59 A61 R62 Q64 R68 H70 R72 L73
Binding residue
(residue number reindexed from 1)
R3 R5 K7 H11 R33 G34 P35 I36 P37 L38 P39 T40 R41 I49 S50 P51 H52 V53 N54 K55 A57 R58 Q60 R64 H66 R68 L69
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0001072 transcription antitermination factor activity, RNA binding
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0005515 protein binding
Biological Process
GO:0002181 cytoplasmic translation
GO:0006412 translation
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0042254 ribosome biogenesis
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome
GO:0008023 transcription elongation factor complex
GO:0015935 small ribosomal subunit
GO:0022627 cytosolic small ribosomal subunit
GO:1990904 ribonucleoprotein complex

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Molecular Function

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Biological Process

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Cellular Component
External links
PDB RCSB:8phj, PDBe:8phj, PDBj:8phj
PDBsum8phj
PubMed38033086
UniProtP0A7R5|RS10_ECOLI Small ribosomal subunit protein uS10 (Gene Name=rpsJ)

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