Structure of PDB 8kab Chain J Binding Site BS01

Receptor Information
>8kab Chain J (length=133) Species: 246196 (Mycolicibacterium smegmatis MC2 155) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
LIKLQIQAGQANPAPPVGPALGQHGVNIMEFCKAYNAATESQRGNVIPVE
ITVYEDRSFTFALKTPPAAKLLLKAAGVQKGSGEPHKTKVAKVTWDQVRE
IAETKKADLNANDIDAAAKIIAGTARSMGITVE
Ligand information
>8kab Chain A (length=3111) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugagacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaaccaggagguuggcuuagaagcagccacccuug
aaagagugcguaauagcucacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccaaaaccaccgugaccgcaccuuucgggguguggcguugg
uggggcugcaugggaccuucguugguaguagucaagcgauggggugacgc
aggaagguagccguaccggucagugguaauaccgggguaagccuguaggg
agucagauagguaaauccgucuggcauauauccugagaggugaugcauag
ccgagugaggcgaauucggugauccuaugcugccgagaaaagccucuagc
gaggacauacacggcccguaccccaaaccaacacagguggucagguagag
aauacuaaggcguacgagugaacuaugguuaaggaacucggcaaaaugcc
cccguaacuucgggagaagggggacccacauggcguguaagccuuuacgg
cccaagcgugaguggguggcacaaaccagugagaagcgacuguuuacuaa
aaacacagguccgugcgaagucgcaagacgauguauacggacugacgccu
gcccggugcuggaagguuaagaggacccguuaacucccuuugggggugaa
gcggagaauuuaagccccaguaaacggcggugguacauccuaagguagcg
aaauuccuugucggguaaguuccgaccugcacgaauggcguaacgacuuc
ucaacugucucaaccauagacucggcgaaauugcacuacgaguaaagaug
cucguuacgcgcggcaggacgaaaagaccccgggaccuucacuacaacuu
gguauuggugcucgauacgguuuguguaggauaggugggagacugugaag
cucacacgccaguguggguggagucguuguugaaauaccacucugaucgu
auugggccucuaaccucggaccguauauccgguucagggacagugccugg
uggguaguuuaacuggggcgguugccuccuaaaauguaacggaggcgccc
aaagguucccucaaccuggacggcaaucagguguugaguguaagugcaca
agggagcuugacugcgagacggacaugucgagcagggacgaaagucggga
cuagugauccggcaccucugaguggaaggggugucgcucaacggauaaaa
gguaccccggggauaacaggcugaucuuccccaagaguccauaucgacgg
gaugguuuggcaccucgaugucggcucgucgcauccuggggcuggagcag
gucccaaggguugggcuguucgcccauuaaagcggcacgcgagcuggguu
uagaacgucgugagacaguucggucucuauccgccgcgcgcgucagaagc
uugaggaaaccugucccuaguacgagaggaccgggacggacgaaccucug
guauaccaguugucccaccaggggcacggcuggauagccacguucggaca
ggauaaccgcugaaagcaucuaagcgggaaaccucuuccaagaccaggcu
ucucacccucuaggagggauaaggccccccgcagaccacgggauugauag
accagaccuggaagccuaguaauaggugcagggaacuggcacuaaccggc
cgaaaacuuac
<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.[[.(((.>>>.....<<<<<<<..<<<)))..]].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<<.........(((((....
.>>>>>>>>>>......<<<<<.<<<<..<<<<<<<))))).>>>>>>><
<<<<<........<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>.>>>>>>.>>>>..
>.>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<....>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<<<<......<<<.
.....>>>.......>>>>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
<<<<<<<<<<.......<<<...<<<..<<<.........>>>..>>>..
.......<<......>>.....>>>...>>>>>>>>>>.>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<
<<<........>>>>>))<<<<<<<<<<........<<.....<<.<<<.
.....<<<<.<<...<<<<<....<<<<<<<<....>>>>>>>>....>>
>>>...>>.>>>>.....>>>.>>.......>>..<<...<<<<<...<<
<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<<...<<<
<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...>>...<
<<......>>>.....>>>>...>>>...>>>>........>>>>>..>>
....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<...
..>>>>..>>>>..<<<<<...<<<<<<<<.<<.........<.....<<
<<<....<<..>>.....>>>>><<<<<<...<<<<....<<<.....>>
>....>>>>..>>>>>>............<<<<<<<<<..<<<.......
..>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.
.<<..<<<<<<<..<<<...<.....<<<<<.<<<<<.....>>>>>..>
>>>>......>..>>>>>>>>..>>>>.......................
...........<.<.......>.>............>>>>>..>>.>>>>
>>>..>..>>.>>>>>>>>>>>>>>>.....>.((..<<<<<......))
>>>>>..<<<<<<<<.<<<.<<......<<<<<<<<<<..<<<<<.<<<.
<<<<<.<<.<<<<<<<<<<<<<.........(.<<<<<..<<.......<
...<<<......>>>...>....>>.........)..>>>>>..>>>>>>
>>>>>>>.>>...<<<.<<<<<<......>>>>>>>>>....>>>>>.>>
>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<
...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>...
.>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>)
))]......<<<<<<<.<<..>>>>..>>>>>>>>>.............>
>>>..>>>>>>.......<<<<.<.<.<<<<...<<.......>>...>>
>>.>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>
>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>..
..<<<<<<<....>>>.>>>>....>>..>>>>>>>>>>>....<<<<<<
<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<
<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>>>>.>>>>>...<
<<....<<<<<...........>>>>>....>>>.>>>>.......>>>>
>>>..<<<<<...>>>>>....<<<<.<<<<.......>>>>.....<<<
.<<<<.<<<....<<<<<....>>>>>...>>>..>>>>..>>>...>>>
>....>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8kab Cryo-EM structures reveal the molecular mechanism of HflX-mediated erythromycin resistance in mycobacteria.
Resolution3.3 Å
Binding residue
(original residue number in PDB)
L10 K12 L13 Q14 P24 P25 P28 A29 H33 K73 K89 G90 S91 G92 E93 P94 H95 K114 D117 L118 N119 A120 N121 D122 A125 K128 I129 G132 T133 R135 S136
Binding residue
(residue number reindexed from 1)
L1 K3 L4 Q5 P15 P16 P19 A20 H24 K64 K80 G81 S82 G83 E84 P85 H86 K105 D108 L109 N110 A111 N112 D113 A116 K119 I120 G123 T124 R126 S127
Gene Ontology
Molecular Function
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
GO:0070180 large ribosomal subunit rRNA binding
Biological Process
GO:0006412 translation
Cellular Component
GO:0005840 ribosome
GO:0015934 large ribosomal subunit
GO:1990904 ribonucleoprotein complex

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Molecular Function

View graph for
Biological Process

View graph for
Cellular Component
External links
PDB RCSB:8kab, PDBe:8kab, PDBj:8kab
PDBsum8kab
PubMed39029461
UniProtA0QS45|RL11_MYCS2 Large ribosomal subunit protein uL11 (Gene Name=rplK)

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