Structure of PDB 8c99 Chain J Binding Site BS01

Receptor Information
>8c99 Chain J (length=132) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDT
GDYIIVLNADKVAVTGNKRTDKVYYQATFEEMIARRPERVIEIAVKGMLP
KGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI
Ligand information
>8c99 Chain A (length=945) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
agcguacacgguggaugcccuggcagucagaggcgaugaaggacgugcua
aucugcgauaagcgucgguaaggugauaugaaccguuauaaccggcgauu
uccgaauggggaaacccaguguguuucgacacacuaucauuaacugaauc
cauagguuaaugaggcgaaccgggggaacugaaacaucuaaguaccccga
ggaaaagaaaucaaccgagauucccccaguagcggcgagcgaacggggag
cagcccagagccugaaucaguguguguguuaguggaagcgucuggaaagg
cgcgcgauacagggugacagccccguacacaaaaaugcacaugcugugag
cucgaugaguagggcgggacacgugguauccugucugaauaugggggauc
cuccaaggcuaaauacuccugacugaccgauagugaaccaguaccgugag
ggaaaggcgaaaagaaccccggcgaggggagugaaaaagaaccugaaacc
guguacguacaagcagugggagcacgcuuaggcgugugacugcggucagc
gacuuauauucuguagcaagguuaaccgaauaggggagccgaagggaaac
cgagucuuaacugggcguuaaguugcaggguauagacccgaaacccggug
aucaccgggagauagcugguucuccccgaaagcuauuuagguagcgccuc
gugaauucaucuccgggggagaauguuaucacgggagacacacggcgggu
gcuaacguccgucgugaagagggaaacaacccagaccgccagcuaagguc
ccaaagucaugguuaagggggcuaaaccaugcaccgaagcugcggcagcg
acgcuuaugcguuguuggguaggggagcguucuguaagccugcgaaggug
ugcugugaggcaugcuggagguaucagaagugcgaaugcugacau
.<<<<<<<<<<<....<<<..<<<<<<<<<<<<<......<<<<<<.[..
(((.>>>.....<<<<<<<..<<<)))..].>>>......>>>>>>>...
>>>....<<<....>>><<<<<<<....>>>>>>>.<<<<<<<<<.....
....>>>>>>>>>.>>...<<<<<<.....<<....>>......>>>>>.
>.........<<....>>...<<<<<.....<<.....>>....>>>>>.
..<<<..<<<<......<<<<<<<<<<<<<.<<<...<<<<<......>>
>>>((......<<<.....))>>>...>>>...>>>>>>>>>>>>>...>
>>>........<.<.<<<<.........>>>>.>.>......<<<<<..>
>>>>..>>>......>>.>>>>>>>.>>...............<<....>
>....>>.........<<<<.....>>>>.............>....>>>
>>>>>>>>.......<<<<..<<<<<......>>>>>..>>>>.<<<<<<
....<<<<<<<<<<<<<..<<<...<<.....>>...>>>...<<....>
>...<........>.......>>>>>>>>>>>>>..<<.<..<<<<<<<.
...>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>>.<<<<
<<<<...<<<<<<<...>>>>.>>>...>>>>>>>.>...<<<<<<<<<.
.......>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<(
((.....<<<<<<....))).....>>>>>>.>>>...>>>>>>><<<<<
<<<<....>>>>>>>>>....>>>>>><<<<<<<...<<<......<<<<
<<<<....>>>>>>>>...>>>..>>>>>.>>.....>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8c99 Cryo-EM captures early ribosome assembly in action.
Resolution3.29 Å
Binding residue
(original residue number in PDB)		K2 T3 K7 P8 T24 G26 R27 T30 R37 K39 H47 V64 T65 K68 K72 Y74 G107 M108 P113 L114
Binding residue
(residue number reindexed from 1)		K2 T3 K7 P8 T24 G26 R27 T30 R37 K39 H47 V64 T65 K68 K72 Y74 G97 M98 P103 L104
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003729 mRNA binding
GO:0003735 structural constituent of ribosome
GO:0008270 zinc ion binding
GO:0048027 mRNA 5'-UTR binding
GO:0070180 large ribosomal subunit rRNA binding
Biological Process
GO:0002181 cytoplasmic translation
GO:0006412 translation
GO:0017148 negative regulation of translation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
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Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8c99, PDBe:8c99, PDBj:8c99
PDBsum8c99
PubMed36797249
UniProtP0AA10|RL13_ECOLI Large ribosomal subunit protein uL13 (Gene Name=rplM)

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