Structure of PDB 7ow7 Chain J Binding Site BS01

Receptor Information

>7ow7 Chain J (length=152) Species: 9606 (Homo sapiens)

VRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDV
TLQKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESN
AELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILT
EK

Ligand information

>7ow7 Chain A (length=3303)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccggggggcccaaguccuucugaucgaggcccag
cccguggacggugugaggccgguagcggcccccggccgggucuucccgga
gucggguugcuugggaaugcagcccaaagcgggugguaaacuccaucuaa
ggcuaaauaccggcacgagaccgauagucaacaaguaccguaagggaaag
uugaaaagaacuuugaagagagaguucaagagggcgugaaaccguuaaga
gguaaacgggugggguccgcgcaguccgcccggaggauucaacccggcgg
cggguccggccgggcggaucugaccgucggcgaccggccgccgccgggcg
cauuuccaccgcggcggugcgccgcgaccggcuccgggacggcugggaag
gcccggcggggaagguggcucggccgaguguuacagcccccccggcagca
gcacucgccgaaucccggggccgagggaggucgccgcgcucuccccccug
ggggggccgggccaccccucccacggcgcgaccgcucggggcggacuguc
cccagugcgccccgggcgggucgcgccgucgggcccggggggccacgcgc
gcgucccgggacggcggagcgagcgcacggggucggcggcgacgucggcu
acccacccgacccgucuugaaacacggaccaaggagucuaacacgugcgc
gagucgggggcucgcacgaaagccgccguggcgcaaugaaggugaaggcc
ggccgaggugggaucccgaggccucucgccgagggcgcaccaccggcccg
ucucgcccgccgcgccggggagguggagcacgagcgcacguguuaggacc
cgaaagauggugaacuaugccugggcagggcgaagccagaggaaacucug
guggagguccguagcgguccugacgugcaaaucggucguccgaccugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccgaag
uuucccucaggauagcuggcgcucucggcaguuuuauccgguaaagcgaa
ugauuagagguaccuauucucaaacuuuaaauggguaagaagcccggcuc
gcuggcguggagccgggcguggaaugcgagugccuagugggccacuuuug
guaagcagaacuggcgcugcgggaugaaccgaacgccggguuaaggcgcc
cgaugccgacgcucaucagaccccagaaaagguguugguugauauagaca
ggaaucaacuagcccugaaaauggauggcgcuggagcgucgggcccauac
ccggccgucgccggcaguccgcggacgcuacgccgcgacgaguaggaggg
ccgcugcggugagccuugaagccuagggcgcgggcccggguggagccgcc
gcaggugcagaucuuggugguaguagcaaauauucaaacgagaacuuuga
aggccgaaguggagaaggguuccaugugaacagcaguugaacauggguca
gucgguccugagagaugggcgagcgccguuccgaagggggccgaucgaaa
gggagucggguucagauccccgaauccggagcggagaugggcgccgcgag
gcguccagugcgguaacgcgaccgaucccggagaagccggcgggagcccc
ggggagaguucucuuuucuuugugaagggcagggcgcccuggaauggguu
cgccccgagagaggggcccgugccuuggaaagcgucgcgguuccggcggc
guccggugagcucucgcuggcccuugaaaauccgggggagaggguguaaa
ucucgcgccgggccguaccuauauccgcagcaggucuccaaggugaacag
ccucuggcauguuggaacaauguagguaagggaagucggcaagccggauc
cguaacuucgggauaaggauuggcucuaagggcuagccgacuuagaacug
gugcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaag
gcccgcggcggguguugacgcgaugugauuucugcccagugcucugaaug
ucaaagugaagaaauucaaugaagcgcggguaaacggcgggagcucucuu
aagguagccaaaugccucgucaucuaauuagugacgcgcaugaauggaug
aacgagauucccacugucccuaccuacuauccagcgaaaccacagccaag
ggaacgggcuuggcggaaucagcggggaaagaagacccuguugagcuuga
cucuagucuggcacggugaagaguuuuucacugacccggugaggcggggg
gcgcuucuggcgccaagcgcccggccgggcgcgacccgcuccggggacag
ugccagguggggaguuugacuggggcgguacaccugucaaacgguaacgc
agguguccuaaggcgagcucagggaggacagaaaccucccguggagcaga
agggcaaaagcucgcuugaucuugauuuucaguacgaauacagaccguga
aagcggggccucacgauccuucugaccuuuuggguuuuaagcaggaggug
ucagaaaaguuaccacagggauaacuggcuuguggcggccaagcguucau
agcgacgucgcuuuuugauccuucgaugucggcucuuccuaucauuguga
agcagaauucaccaagcguuggauuguucacccacuaauagggaacguga
gcuggguuuagaccgucgugagacagguuaguuuuacccuacugaugaug
uguuguugccaugguaauccugcucaguacgagaggaaccgcagguucag
acauuugguguaugugcuuggcugaggagccaauggggcgaagcuaccau
cugugggauuaugacugaacgccucuaagucagaaucccgcccaggcgga
acgauacggcagcgccgcggagccucgguuggccucggauagccgguccc
cgggguccggugcggagugcccuucguccugggaaacggggcgcggccgg
agaggcggccgcccccucgcccgucacgcaccgcacguucguggggaacc
uggcgcuaaaccauucguagacgaccugcuugggucgggguuucguacgu
agcagagcagcgcugcgaucuauugaaagucagcccucgacacaaggguu
ugu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..<<<...<<<<...<<<<<<<<.........>>>>.>>>>...
..>>>>...<<<<<<<<<.<<<<<<<<....<<<<......>>>>.....
((......<<<.....))>>>...>.>>>>>>>....>>>>>>>>>....
...<<<<<<<........>>>>>>>.....<<<<<..((((((>>>>>..
>>>..................................<<....>>.....
..........<<<<....>>>>............................
......<<<<<<<<..<<<.<....<<<<<<<<.<<<.....<<<<<<<<
<<<..<<<<<<..<<<<.<<.>>>>>>.>>>...>>>>>>>>>>>>>>..
....>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<<<<<<...<<..
<<<....<<<<......<<<<<..>>>>>.......>>>>...>>>....
....>>>>>>..>>>>>>>>>>>............<<<<<..<<<<<<..
>>>>>>.<<<<<<.<<<<.<<<<<<<<<<<.<<<<<<<<<<<<.<<<<..
..>>>>.>>>>>>>>>>>>.>.>>>>>>>.>>>...>>>>>>>>.>>..<
<<<<<<..>>>>>.>>.....>>>>>......>>.>>>>>....>>>>..
.>>>>>>>>..<<<<........>>>><<<<<<...<<<<<<<<<<<<<<
..<<<...<<<<........>>>>.....>>>...<<....>>...<<<.
.>>>..<<<<<...<<<..<<<.....>>>..>>>....>>>>>.<<<<<
<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>>.<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<.<<<<<<<...<<<.<<<<<<<<<...<<<....
<<<..<.<<<..>>>.>..>>>..>.>>...>>>>>>>...<<<<<<<<<
.........>>>>>>>>>....>>>>>>>>>>>>..>>..<<<<.<<<<<
.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(((
.......<<<<<.....<<..........<<<.<<<<<<<<<<.......
...>>>>>>>>>>>>>..........))).>>...>>>>>..>>>...>>
>>>>><<<<<<.<<<................>>>>>>>>>....>>>>>>
...<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>
>>>>........>>>>>><<.....<<.....<<<<<<.......>>>>>
>.<<<.....<<<<<<<<...<<<<<<...<<.....>>.>>>>>>....
((<<<<<<...<<....<<<....>>>.................>>....
>>>..<<<<<........>>>>>))<<<<.<<<<<..<<<<<<<<....>
>>>>>>>..<<<....>>>...<..<<<<<<<...<<<<<<<<<<<<<<<
<<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.<<<<.....<<<..
...>>>.....>>>>>>>>..>>>>>.....<<<<<<<.......>>>>>
>>>>>>.>.>>>>>>>>>>>>.........>>>>>>>..>..........
>>.>>>.>>>>.>>>..>>>....<<......>>>>>>>.<<<<.....>
>>>..>>>....<<<<.....<<<<<<<.<<.........<.....<<<<
<...............>>>>><<.<<<<<<<<<..>>>..>>>>>>.>>.
...........<<<<<<<<<..<<<.........>>>..<<<<<<<....
<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....<<
<.....<<<<....>>>>.....>>>.>>>>..>>>>><<<<..>>>>..
......<...........<<<<<.......>>>>>.....>.....>>..
..>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<
........>>>>>....<<<<<..<<<.<<......<<<<.<<<<<<..<
<<<<.<<<<<<<<<.<<<<<<<..>>>>>>>...<<<<<.....<<<<..
................<<<<<<<..>>>>>>>..>>>>..>>>>>....>
>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>
>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.
(((>>....>>>>>>>...<<<..<<<<.......>>>>..>>><<<<..
..>>>>>>)))].....<<<<<<<..<<<..)))))).>>>>>>>>>>..
.......>>>>..>>>>>>.......<<.<...<<<<<<...<<......
.>>...>>>>>>....>.>.>.......<<<<<.<<<<<<..<.<.<<<<
<......>>>>>...>.>.<<<.........>>>.....>>>>>..>...
>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....
.<<<<<<.<..<<<....<<<<<.................>>>>>..<<<
<....<<<<....<<.<<<<<<.]]]..>>>>..>>.>>...>>>>...>
>>><<<<<...<<<<<...........>>>>>...>>>>>.>>>...>.>
>>>>>.....<<<<<<..<<..<<<<....<<<........<<<<<.<<<
..>>>.>>>>>.......>>>...<<<..<<<<....<<<<.<<<<<<<.
.....>>>>>>>>>>>...>>>>..>>>..............>>>>..>>
.>>>>>><<<<<.<.............>..<<.<<<<<<<.<<.....<<
<<.<..<<<<..>>>>..>>>>>....>>....>>>>.>>>.>>..>>>>
>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ow7 Structure of human EIF6-bound large ribosomal subunit
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): V2 R3 Y4 K16 S17 R18 G19 S20 R23 H25 K27 R30 Q34 K37 K43 R62 Y63 N64 G65 G66 G68 R69 A71 Q72 K74 Q75 W78 Q80 R82 W83 P84 K85 K86 N97 S100 N101 Q118 V119 K121 R127 T129 Y130 R131 A132 H133 G134 R135 I136 N137 P138 Y139 M140 S141 S142
• Binding residue (residue number reindexed from 1): V1 R2 Y3 K15 S16 R17 G18 S19 R22 H24 K26 R29 Q33 K36 K42 R61 Y62 N63 G64 G65 G67 R68 A70 Q71 K73 Q74 W77 Q79 R81 W82 P83 K84 K85 N96 S99 N100 Q117 V118 K120 R126 T128 Y129 R130 A131 H132 G133 R134 I135 N136 P137 Y138 M139 S140 S141

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ow7, PDBe:7ow7, PDBj:7ow7
• PDBsum: 7ow7
• UniProt: P18621|RL17_HUMAN Large ribosomal subunit protein uL22 (Gene Name=RPL17)