Structure of PDB 7ohr Chain J Binding Site BS01

Receptor Information

>7ohr Chain J (length=145) Species: 559292 (Saccharomyces cerevisiae S288C)

NNTKAMKHALERVQLPWKKHSFQEHQSVTSETNTDEHIKDIYDDTERELA
FYKQSLDAVLVARDELKRLKVPFKRPLDYFAEMVKSDEHMDKIKGKLIEE
ASDKKAREEARRQRQLKKFGKQVQNATLQKRQLEKRETLEKIKSL

Ligand information

>7ohr Chain 1 (length=2373)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaggccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaacgagguuggggccucgcaagaggugcacaaucgaccgauccugagga
uggauuugguaagagcauagcuguugggacccgaaagauggugaacuaug
ccugaauagggugaagccagaggaaacucugguggagcucgguucgacgu
ucgaucgugaauuggguauagggcgaaagacaaccaucuaguagcugguu
ccugccgaaguuucccuaggauagcagaagcucguaucaguuuuaugagu
aagaaugaagagcuuuuagugggccauuuuugguaagcagaacuggcgau
gcgggaugaaccgaacguagaguuaaggugccggaauacacgcucaucag
acacaaaagguguuaguucaucuagacagccggacgguggccauggaagu
cggaauccgcuaaggaguguguaacaacucaccggccgaaugaacuagcc
cugaaaauggauggcgcucaagcguguuaccuauacucuaccgucagggu
ugauaugaugcccugacgaguaggcaggcguggaggucagugacgaagcc
uagaccguaaggucgggucgaacggccucuagugcagaucuuggugguag
uagcaaauauucaaaugagaacuuugaagacugaaguggggaaagguucc
acgucaacagcaguuggacguggguuagucgauccuaagagauggggaag
cuccguuucaaaggccugauuuuaugcaggccaccaucgaaagggaaucc
gguuaagauuccggaaccuggauauggauucuucacgguaacguaacuga
auguggagacgucggcgcgagcccugggaggaguuaucuuuucuucuuaa
cagcuuaucaccccggaauugguuuauccggagauggggucuuauggcug
gaagaggccagcacugcuggcuccggugcgcuugugacggccgugaaaau
ccacaggaaggaauaguuuucaugccaggucguacugggucuccaaggug
aacagccucuaguugauagaauaauguagauaagggaagucggcuguccc
uaucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauc
agcggggacccuguugagcuugacucuaguuugacauuauugucaggugg
ggaguaaaguuaccacagggauaauggcuuguggcagucaagcguucagc
gacauugcucggcucuuccuaucauaccgaagcagaauucgguaagcguu
ggauuguucacccacuaauagggaacguggcuggguagaccgucgugaga
cgguuaguuuuacccuacugaugaauguuaccgcaauaguaauugaacuu
aguacgagaggaacaguucauucggauaauugguuuuugcggcugucuga
ucaggcaugccgcgaagcuaccauccgcuggauuauggcugaacgccucu
aagucagaauccaugcuagaacgcggugauuucuuugcuccacacaauau
agauggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggc
uagcaacggugcacuuggcggaaaggccuugggugcuugcuggcgaauug
caaugucauuuugcguggggauaaaucauuuguauacgacuuagauguac
aacgggguauuguaagcaguagaguagccuugguuacgaucugcugagau
uaagccuuuguugucugauuugu

..........................<<<<<<.....<......>.....
.>>>>>.>...<.....<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>......
..>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......
>>>>.((......<<<.....))>>>....>>>>>>>>>..>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<<..>>>>>>..>>>....>............................
.<<....>>...............<<<<....>>>>..............
....................<<<<<.<<<<<......<<<<<<<.<<<<<
<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>....
...>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>.
....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<
<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.....
......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<......
.>>>.>>>>>....>>>>>>.>>>>>>..<<.<..<<<<<<<...<<<<<
<<<<....<<<<...<<<<<<<....>>>>>>>...>>>>...<<<....
.>>>........>>>>>>>>>...........>>>>>>>.....>.>>..
<<<<<<....<...........><<<<<<<<<....<<<.<<<<<<..>>
>>.>>>>>..>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>...
.<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<
.......<<<.<<<<<<<<<<.......<<<<<.<<<...<<........
.>>...>>>.........<<......>>...>>>>>...>>>>>>>>>>>
>>..........))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<
.........>>>>>>>>>....>>>>>>..<<<<<<<..<<......<<<
<.<<<<....>>>>>>>>...>>.>>>>>>>........>>>>>><<...
..<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<
<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<<...
.>>>........<<<<<<........>>>>>>....>>....>>...<<<
<<........>>>>>))<<<<.<<<<<<<.....<<<....>>>...<..
.<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..(((..>>>>>><...
<<<<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>
....><<<<<<<...>>>>>>>>>>>...>>>>>>>.>>>>........>
>>>>>...>......>.>>.>>>>>>>>>>>>..>>...>>>>>.<<<<.
....>>>>..>>>>..<<<<.....<<<<<<<.<<............>>.
>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<
<<..<<..<<<<<.<..<<<<..<<<<.<<<<<<<<....>>>>>>>>..
>>>>..>>>>....>>>>>>............<<<<<....<<.....>>
....>>>>>.<<...<<<<<..<.<<<<<<<......>>>>>>>...>.<
<<.........>>>.....>>>>>......>>.....<<<<<<<....>>
>>>>>.........>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<.
..............>>>>>>.><<<<<...<<<<...<<<<<<.<<<<))
).>>>>..>>>>>>..>>>>..>>>>>.<<<<...<<<<<..........
.>>>>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<
....<<..........<<<<<<.....>>>>>>......>>.......<<
<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>......
.......>>.>>>.>>>>>>..<<<<<.<.............>.....<<
<<<.<<<.<<<....<<<<....<..<<<..>>>..>...>>>>...>>>
....>>>..>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohr Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 4.72 Å
• Binding residue (original residue number in PDB): K300 R302 A305 R306 R307 R309 K312 K313 K316 Q319 N320 R326 Q327 R331
• Binding residue (residue number reindexed from 1): K105 R107 A110 R111 R112 R114 K117 K118 K121 Q124 N125 R131 Q132 R136

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0005515 protein binding
• GO:0042802 identical protein binding

Biological Process

• GO:0000280 nuclear division
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor
• GO:0034399 nuclear periphery

External links

• PDB: RCSB:7ohr, PDBe:7ohr, PDBj:7ohr
• PDBsum: 7ohr
• PubMed: 34813592
• UniProt: P36049|EBP2_YEAST rRNA-processing protein EBP2 (Gene Name=EBP2)