Structure of PDB 7msm Chain J Binding Site BS01

Receptor Information

>7msm Chain J (length=146) Species: 83332 (Mycobacterium tuberculosis H37Rv)

PTYAPKAGDTTRSWYVIDATDVVLGRLAVAAANLLRGKHKPTFAPNVDGG
DFVIVINADKVAISGDKLQHKMVYRHSGYPGGLHKRTIGELMQRHPDRVV
EKAILGMLPKNRLSRQIQRKLRVYAGPEHPHSAQQPVPYELKQVAQ

Ligand information

>7msm Chain A (length=3118)

aagugucuaagggcgcaugguggaugccuuggcaucgagagccgaugaag
gacgugggaggcugcgauaugccucggggagcugucaaccgagcguggau
ccgaggauuuccgaauggggaaacccagcacgagugaugucgugcuaccc
gcaucugaauauauagggugcgggagggaacgcggggaagugaaacaucu
caguacccguaggaggagaaaacaauugugauuccgcaaguaguggcgag
cgaacgcggaacaggcuaaaccgcacgcauggguaaccggguagggguug
ugugugcgggguugugggaggauaugucucagcgcuacccggcugagagg
cagucagaaagugucgugguuagcggaaguggccugggauggucugccgu
agacggugagagcccgguacgcgaaaacccggcaccugccuaguaucaau
ucccgaguagcagcgggcccguggaauccgcugugaauccgccgggacca
cccgguaagccuaaauacuccucgaugaccgauagcggauuaguaccgug
agggaauggugaaaaguaccccgggaggggagugaaagaguaccugaaac
cgugugccuacaauccgucagagccuccuuuuccucuccggaggagggug
gugauggcgugccuuuugaagaaugagccugcgagucagggacaugucgc
aagguuaacccgugugggguagccgcagcgaaagcgagucugaauagggc
gacccacacgcgcauacgcgcgugugaauaguggcguguucuggacccga
agcggagugaucuacccauggccagggugaagcgcggguaagaccgcgug
gaggcccgaacccacuuagguugaagacugaggggaugagcuguggguag
gggugaaaggccaaucaaacuccgugauagcugguucuccccgaaaugca
uuuaggugcagcguugcgugguucaccgcggagguagagcuacuggaugg
ccgauggguuacugacgucagccaaacuccgaaugccgugguguaaagcg
uggcagugagacggcgggggauaagcuccguacgucgaaagggaaacagc
ccagaucgccggcuaaggcccccaagcgugugcuaagugggaaaggaugu
gcagucgcaaagacaaccaggagguuggcuuagaagcagccacccuugaa
agagugcguaauagcucacuggucaagugauugugcgccgauaauguagc
ggggcucaagcacaccgccgaagccgcggcacauccaccuuguggugggu
guggguaggggagcgucccucauucagcgaagccaccgggugaccggugg
uggagggugggggagugagaaugcaggcaugaguagcgacaaggcaagug
agaaccuugcccgccgaaagaccaaggguuccugggccaggccaguccgc
ccagggugagucgggaccuaaggcgaggccgacaggcguagucgauggac
aacggguugauauucccguacccgugugugggcgcccgugacgaaucagc
gguacuaaccacccaaaaccggaucgaucacuccccuucgggggugugga
guucuggggcugcgugggaacuucgcugguaguagucaagcgaaggggug
acgcaggaagguagccguaccagucagugguaacacuggggcaagccggu
agggagagcgauaggcaaauccgucgcucacuaauccugagaggugacgc
auagccgguugaggcgaauucggugauccucugcugccaagaaaagccuc
uagcgagcacacacacggcccguaccccaaaccgacacagguggucaggu
agagcauaccaaggcguacgagauaacuaugguuaaggaacucggcaaaa
ugcccccguaacuucgggagaagggggaccggaauaucgugaacacccuu
gcggugggagcgggauccggucgcagaaaccagugaggagcgacuguuua
cuaaaaacacagguccgugcgaagucgcaagacgauguauacggacugac
gccugcccggugcuggaagguuaagaggacccguuaacccgcaaggguga
agcggagaauuuaagccccaguaaacggcggugguaacuauaaccauccu
aagguagcgaaauuccuugucggguaaguuccgaccugcacgaauggcgu
aacgacuucucaacugucucaaccauagacucggcgaaauugcacuacga
guaaagaugcucguuacgcgcggcaggacgaaaagaccccgggaccuuca
cuacaacuugguauugauguucgguacgguuuguguaggauaggugggag
acugugaaaccucgacgccaguuggggcggagucguuguugaaauaccac
ucugaucguauugggcaucuaaccucgaacccugaaucggguuuagggac
agugccuggcggguaguuuaacuggggcgguugccuccuaaaauguaacg
gaggcgcccaaagguucccucaaccuggacggcaaucagguggcgagugu
aaaugcacaagggagcuugacugcgagacuuacaagucaagcagggacga
aagucgggauuagugauccggcacccccgaguggaaggggugucgcucaa
cggauaaaagguaccccggggauaacaggcugaucuuccccaagagucca
uaucgacgggaugguuuggcaccucgaugucggcucgucgcauccugggg
cuggagcaggucccaaggguugggcuguucgcccauuaaagcggcacgcg
agcuggguuuagaacgucgugagacaguucggucucuauccgccgcgcgc
gucagaaacuugaggaaaccugucccuaguacgagaggaccgggacggac
gaaccucuggugcaccaguugucccgccaggggcaccgcuggauagccac
guucggucaggauaaccgcugaaagcaucuaagcgggaaaccuucuccaa
gaucagguuucucacccacuuggugggauaaggccccccgcagaacacgg
guucaauaggucagaccuggaagcucaguaauggguguagggaacuggug
cuaaccggccgaaaacuu

<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.((..((.>>>.....<<<<<<<..<<<))...)).>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<<..>>>..<<<<<<.....<<.....>
>....>>>>>>..<<<....<<<<<<<<<<......<<((((((>>...>
>>>>>>>>>......<<<<<<<<<<..<<<<<<.)))))).>>>>>><<<
<<<.......<<<<<<.<<<<.<<<.....<<<<......>>>>.((...
...<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>.>>>>>..>
>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<<<
<>>>>>..>>>......>>.>>>>>>>.>>...............<<...
.>>....>>.........<<<<....>>>>..................>>
>>>>>>>>>..<....<<<<..<<<.<<<..<...........>.>>>.>
>>..>>>>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<
..<<<...<<<....>>>...>>>...<<....>>..<<<<.....>>>>
.......<<<<........>>>>.......>>>>>>>>>>>>>..<<.<.
.<<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>>>>>>.
....>>>...<<<.<<<<<........>>>>>>>>....>>>>>>>>>..
.<<<.....>>>......>>>>>>...>..>.>>..<<<<<<....<<<<
<....>>>>><<<<<<<<<....<<<<<<<<.....<<<....<<<.<<<
<....<.....>....>>>>.>>>..>>.>.....>>>>>>>>....>>>
>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<......>
>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<<
<<<<<<<........<<<<<.<<.....<.........>.....>>....
.....<<......>>...>>>>>....>>>>>>>>>.>>...........
)))......>>>>>>.>>>...>>>>>>><<<<<<<<<<....>>>>>>>
>>>....>>>>>><.<<<<<<<<<.......<<<<<<<<....>>>>>>>
>....>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<..
.....>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>
>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>..
.<<<<<........>>>>>))<<<<<<<<<.<<.....<<<<....<<<<
<.......<<<<.<....<<<<<....<<<..<<<......>>>..>>>.
..>>>>>....>.>>>>......>>>>>......>>>>.<<..<<<<<<.
..<<<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<...
.<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>....>>>...>>
...<<<......>>>.....>>>>...>>>...>>>>........>>>>>
>.>>..>>.>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<
<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.............
..<<<<<.....<..>......>>>>><<<<<<...<<.....<.<<...
..>>..>......>>>>>>>>............<<<<<<<<<..<<<...
......>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<
<<<.<<<..<<<<<<<..<<<<.<<<....<<<<..<<<<....>>>>..
.>>>>....>>>.>>>>>>>>>..>>>>><<<<<<.......>>>>>>..
......<...........<<<<<<.....>>>>>>.....>....>>>>>
..>>.>>>>>>>.....>>.>>>>>>>>.>>>>>>.....>.((..<<<<
<......))>>>>>>.<<<<<<<<.<<<.<<......<<<<<<<<<<..<
<<<<..<<.<<<<<.<<<<<<<<<<<<<<<<...........<<<<<...
..............................................>>>>
>..>>>>>>>>>>>>>>>>...<<<.<<<<<<......>>>>>>>>>...
.>>>>>.>>...>>>>>..<<<<.....>>>><<<<<<...........>
>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[
.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<..
..>>>.>>)))]......<<<<<<<.<<..>>>>..>>>>>>>>>.....
........>>>>..>>>>>>.......<<<<...<.<<<<...<<.....
..>>...>>>>.>....>>>.>.......<<<<<.<<<<<<..<<<<<<<
<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>..
.>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>>>>>.
...<<<<<<<...<<<...<<<<<<<...............>>>>>>>.<
<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>>>>.
>>>>>...<<<....<<<<<...........>>>>>....>>>..>>>..
.....>>>>>>>..<<<<.....>>>>....<<<<.<<<<.......>>>
>.....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.
>>>...>>>>....>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7msm Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis.
• Resolution: 2.79 Å
• Binding residue (original residue number in PDB): T3 K7 A8 W15 G26 R27 V30 R37 P46 N47 K68 K72 Y75 H77 S78 Y80 P81 G82 L84 H85 R99 E102 A104 G107 M108 P110 N112 R113 L114 R120 R123 H132 A134 Q135 K143 Q144 Q147
• Binding residue (residue number reindexed from 1): T2 K6 A7 W14 G25 R26 V29 R36 P45 N46 K67 K71 Y74 H76 S77 Y79 P80 G81 L83 H84 R98 E101 A103 G106 M107 P109 N111 R112 L113 R119 R122 H131 A133 Q134 K142 Q143 Q146

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7msm, PDBe:7msm, PDBj:7msm
• PDBsum: 7msm
• PubMed: 35064151
• UniProt: A0A0T9D5H2