Structure of PDB 7a09 Chain J Binding Site BS01

Receptor Information

>7a09 Chain J (length=576) Species: 9606 (Homo sapiens)

KLTRIAIVNHDKCKPKKCRQECKKSCPVVRMGKLCIEVTPQSKIAWISET
LCIGCGICIKKCPFGALSIVNLPSNLEKETTHRYCANAFKLHRLPIPRPG
EVLGLVGTNGIGKSTALKILAGKQKPNLGKYDDPPDWQEILTYFRGSELQ
NYFTKILEDDLKAIIKPQYVDQIPKAAKGTVGSILDRKDETKTQAIVCQQ
LDLTHLKERNVEDLSGGELQRFACAVVCIQKADIFMFDEPSSYLDVKQRL
KAAITIRSLINPDRYIIVVEHDLSVLDYLSDFICCLYGVPSAYGVVTMPF
SVREGINIFLDGYVPTENLRFRDASYKYPGMKKKMGEFELAIVAGEFTDS
EIMVMLGENGTGKTTFIRMLAGRLKPDEGGEVPVLNVSYKPQKISPKSTG
SVRQLLHEKIRDAYTHPQFVTDVMKPLQIENIIDQEVQTLSGGELQRVAL
ALCLGKPADVYLIDEPSAYLDSEQRLMAARVVKRFILHAKKTAFVVEHDF
IMATYLADRVIVFDGVPSKNTVANSPQTLLAGMNKFLSQLEITFRRDPNN
YRPRINKLNSIKDVEQKKSGNYFFLD

Ligand information

>7a09 Chain 2 (length=1721)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
ccgcggcgacgacccauucgaacgucugcccuaucaacuuucgaugguag
ucgccgugccuaccauggugaccacgggugacggggaaucaggguucgau
uccggagagggagccugagaaacggcuaccacauccaaggaaggcagcag
gcgcgcaaauuacccacucccgacccggggagguagugacgaaaaauaac
aauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaaau
ccuuuaacgaggauccauuggagggcaagucuggugccagcagccgcggu
aauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcucg
uaguuggaucuugggagcgggcgggcgguccgccgcgaggcgagccaccg
cccguccccgcccucucggccgaagcguuuacuuugaaaaaauuagagug
uucaaagcaggccgccuggauaccgcagcuaggaauaauggaauaggacc
gcgguucuauuuuguugguuuucggaacugaggccaugauuaagagggac
ggccgggggcauucguauugcgccgcuagaggugaaauucuuggaccggc
gcaagacggaccagagcgaaagcauuugccaagaauguuuucauuaauca
agaacgaaagucggagguucgaagacgaucagauaccgucguaguuccga
ccauaaacgaugccgaccggcgaugcggcggcguuauucccaugacccgc
cgggcagcuuccgggaaaccaaagucuuuggguuccggggggaguauggu
ugcaaagcugaaacuuaaaggaauugacggaagggcaccaccaggagugg
agccugcggcuuaauuugacucaacacgggaaaccucacccggcccggac
acggacaggauugacagauugauagcucuuucucgauuccguggguggug
gugcauggccguucuuaguugguggagcgauuugucugguuaauuccgau
aacgaacgagacucuggcaugcuaacuaguuacgcgaccggucggcguaa
cuucuuagagggacaaguggcguucagccacccgagauugagcaauaaca
ggucugugaugcccuuagauguccggggcugcacgcgcgcuacacugacu
ggcucagcgugugccuacccuacgccggcaggcgcggguaacccguugaa
ccccauucgugauggggaucggggauugcaauuauuccccaugaacgagg
aauucccaguaagugcgggucauaagcuugcguugauuaagucccugccc
uuuguacacaccgcccgucgcuacuaccgauuggaugguuuagugaggcc
cucggaucggccccgccggggugcccuggcggagcgcugagaagacgguc
gaacuugacuaucuagaggaaguaaaagucguaacaagguuuccguaggu
aaaccugcggaaggaucauua

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<<.........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<.....>.>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<.>>>>>>>.>>.>>>...>>..>.>>>.<<<....<<<....<<<<<<<
.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>...
.>>>.>>.<<.<<<..........>>>.>>...<<....>>.....>>>>
>>.........<<<....<<<.....>>>..>>>..>...>>.>.....<
<<<<<<<<.<........>..>>>>>>.>>>......<<..<........
...>..>>.........<<<<<((......<<<<.....<<..))>>...
....>>>>.>>>>>..>>>>>>.>>>.........<.<((.....<.<<.
..<<<.<<.....<<.<.<<<<<<<<<<<.<<<<....>>>>....>>>>
>>>>>>>>..>>...<......>.....<<<<<<.<.......<<...<.
......>.<<<<.>>>>...>>......>.>>>..>>>........<.<<
.<<<<<..................>.>>>>.>>.>....>>....<<<<<
<..<...<<<<..<<..<<<<<<<<...<<<......>>>......>>>>
>>>>..>>.......<<....>>...>>>>..>..>>>.>>>...>>>..
.>>.>....<<<<<<<...<...<<<<.........>>>>...>>>>>>>
>..........<<<.<<.<<<..<.<<<<<<.<<<<<....>>>>>>>>>
>>.>..>>>...<<..))>>...>>.....>>>.>.>.<<<......<<<
<....>>>>....>>>..)))).]<<<<<.<<<<<<<..<<..<<<<<..
<<<.<<<<<<......<<........>>..........<<<<<.<....<
<<<<<.......<<.<<<........>>>.>>.....>>>>>>...<<.<
<<..<<.<<<<<<....<<<.<<<<<....>>>...<<<......>>>..
.>>.>>>....<<<...<...<<<<..<<<<.<<<<<<..>>>.>>>.>>
>>..>>>>..>.....<<<<<.....>>>>>........>>>....>>>.
>>>.....>>>>>>>.....>.>>>>>...>>.>>>>.>>>.....<<.<
<<........<.......<<<.<<<<....>>>>.>>>....>.......
..>>>.>>......<...<.<<<<<..........>>>>>>....>....
.>>>>>.....<<<<<<<<.......>>>>>>>>......>>...>>>>>
>>>>>.>>.......<<.<..<<<<.<<....<<<<<<<<.<<<..<<<<
.<<...<<<<<.<.................>....>>>>>...>>.>>>>
..>>>.>>>>>>>>...>>.>>>>...>.>>.........<<<<<<<<<.
...>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7a09 A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): R7 R22 K63 R101 R148 G149 F285 M301 F303 R306 N310 T319 E320 N321 R567 R576 N578 K584
• Binding residue (residue number reindexed from 1): R4 R19 K60 R98 R145 G146 F282 M298 F300 R303 N307 T316 E317 N318 R545 R554 N556 K562

Enzymatic activity

• Enzyme Commision number: 3.6.5.-

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005506 iron ion binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0017111 ribonucleoside triphosphate phosphatase activity
• GO:0043024 ribosomal small subunit binding
• GO:0043273 CTPase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding
• GO:0060698 endoribonuclease inhibitor activity

Biological Process

• GO:0000054 ribosomal subunit export from nucleus
• GO:0006413 translational initiation
• GO:0006415 translational termination
• GO:0006417 regulation of translation
• GO:0032790 ribosome disassembly
• GO:0060702 negative regulation of endoribonuclease activity
• GO:0072344 rescue of stalled ribosome

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0005829 cytosol
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome

External links

• PDB: RCSB:7a09, PDBe:7a09, PDBj:7a09
• PDBsum: 7a09
• PubMed: 33289941
• UniProt: P61221|ABCE1_HUMAN ATP-binding cassette sub-family E member 1 (Gene Name=ABCE1)