Structure of PDB 6ylh Chain J Binding Site BS01

Receptor Information

>6ylh Chain J (length=168) Species: 4932 (Saccharomyces cerevisiae)

QNPMRDLKIEKLVLNISVGESGDRLTRASKVLEQLSGQTPVQSKARYTVR
TFGIRRNEKIAVHVTVRGPKAEEILERGLKVKEYQLRDRNFSATGNFGFG
IDEHIDLGIKYDPSIGIFGMDFYVVMNRPGARVTRRKRCKGTVGNSHKTT
KEDTVSWFKQKYDADVLD

Ligand information

>6ylh Chain 1 (length=3039)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaac
aaguacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaag
uacgugaaauuguugaaagggaagggcauuugaucagagccagcaucagu
uuugguggcaggauaaauccauaggaauguagcuugccucgguaaguauu
auagccugugggaauacugccagcugggacugaggacugcgacguaaguc
aaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggacca
aggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaa
ugaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgau
ccugaugucuucggauggauuugaguaagagcauagcuguugggacccga
aagauggugaacuaugccugaauagggugaagccagaggaaacucuggug
gaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguaua
ggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuu
cccucaggauagcagaagcucguaucaguuuuaugagauuagagguuccg
gggucgaaaugaccuugaccuauucuguaagaaguccuuguuugaacgug
gacgaaugaagcuuuuagugggccauuuuugguaagcagaacuggcgaug
cgggaugaaccgaacguagaguuaaggugccggaauacacgcucaucaga
caccacaaaagguguuaguucaucuagacagccggacgguggccauggaa
gucggaauccgcuaaggaguguguaacaacucaccggccgaaugaacuag
cccugaaaauggauggcgcucaagcguguuaccuauacucuaccgucagg
guuaugcccugacgaguaggcaggcguggaggucagugacgaagccuaga
ccguaaggucgggucgaacggccucuagugcagaucuuggugguaguagc
aaauauucaaaugagaacuuugaagacugaaguggggaaagguuccacgu
caacagcaguuggacguggguuagucgauccuaagagauggggaagcucc
guuucaaaggccuggcaggccaccaucgaaagggaauccgguuaagauuc
cggaaccuggauauggauucuucacgguaacguaacugaauguggagacg
ucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuaucac
cccggaauugguuuauccggagauggggucuuauggcuggaagaggccag
caccuuugcuggcuccggugcgcuugugacggcccgugaaaauccacagg
aaggaauaguuuucaugccaggucguacugauaaccgcagcaggucucca
aggugaacagccucuaguugauagaauaauguagauaagggaagucggca
aaauagauccguaacuucgggauaaggauuggcucuaagggucggcgauc
aacuuagaacugguacggacaaggggaaucugacugucuaauuaaaacau
agcauugcgauggucagaaagugauguugacgcaaugugauuucugccca
gugcucugaaugucaaagugaagaaauucaaccaagcgcggguaaacggc
gggaguacucucuuaagguagggauuaacgagauucccacugucccuauc
uacuaucuagcgaaaccacagccaagggaacgggcuuggcagaaucagcg
gggaaagacccuguugagcuugacucuaguuugacauugugaagagacau
agaggguguagaauaagugggagcuucggcgccagugaaauaccacuacc
uuuauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuucc
acguucuagcauucaaggucccauucggggcugauccggguugaagacau
ugucagguggggaguuucacaucuguugcagaugucuaaggggggcucau
ggagaacagaaaucuccaguagaacaaaggguaaaagcccccuugauuuu
gauuuuugugaauacaaaccaugaaaguguggccccuuuagucccucgga
auuugaggcuagaggaaaguuaccacaggguggcuuguggcagucaagcg
uucauagcgacauugcuuuuugauucuucgaucggcucuuccuaucauac
cgaagcagaauucgguaagcguuggauuguucacccacuaauagggaacg
ugagcugguagaccgucgugagacagguuaguuuuacccuacugaugaau
guuaccgcaauaguaauugaacuuaguacgagaggaacaguucauucgga
uaauugguuuuugcggcugucugaucaggcauugccgcgaagcuaccauc
cgcuggauuauggcugaacgccucuaagucagaauccaugcuagaacgcg
gugauuugcuccacacaaauagauggauacgaauaaggcguccuuguggc
gucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccuu
gggugcuugcuggcgaauugcaaugucauuugcguggggauaaaucauuu
guauacgacuuagauguacaacgggguauuguaagcaguagaguagccgu
uacgaucugcugagauuaagccuuuguugucugauuugu

..........................<<<<<<.....<......>.....
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>......
.>>....<<<<<<.<<<<<.<<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>>..>>>>>>...
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((
(((..<<<<<<)))))..]]].............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<.<<<<<...<<<<<<<.<<<<
<<<<<<<<<<<.....<<<<<<<<<......<<<<<......>>>>>...
....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>
.....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<
<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<
<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<
<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>>..<<.<.
.<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.
....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>.
..<<.......>>......>>>>>>...>..>.>>..<<<<<<......<
<......>>..<<<<<<<<<....<<<.<<<<<<..<<<....<.<....
<<.<<.....>>>>..>..>.>>>..>>>>...<<<<.<<<....>>>.>
>>>.>>>>>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....
<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<......<.
.........<<<.<<<<<<<<<<.......<<<<<.<<....<<......
...>>....>>.........<<......>>...>>>>>...>>>>>>>>>
>>>>..........))).>....>>>>>>.>>>>..>>>>>>><<<<<<<
<<...>>>>>>>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<
<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<.....<<
.....<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<
...<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>
........<<<<<<.>>>>>>....>>....>>...<<<<<........>
>>>>))<<<<.<<<<<<<.....<<<....>>>...<...<<<<<<...<
<<<.<<<<<<<.<<<<<<<<<<..{{{..>>>>>><...<<<<<....<<
<<<<....<<<.....>>>....>>>>>>.....>>>>>....><<<<<<
<......>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>..
.>......>>>..>>>>>>>>>>>>..>>.....<<......>>>>>>>.
<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.........<
.....<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<.>>>>>
..>>>>>>.>>............<<<<<<<<<..<<<.........>>>.
.<<<<<<<....<<<<.....>>>>......>>>>>>>.......<<<..
<<<<<<....<<<.....<<<<....>>>>.....>>>.>>>>..>>>>>
<<<.<...>.>>>...............>>.>>>>>>>..>..>>.>>>>
>>>..>>>>>>....>>....<<<<<........>>>>>....[[[[[..
{{{.....[[[[[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<<<<<
<<<<<..........<<<<<......................>>>>>.>>
>>>>>.>>>>>>>>>>...<<<<<.....<<<<..<<<<<......>>>>
>...............<<<<<<....>>>>>>..>>>>..>>>>>.....
>>>>>>>>..>>>>>........................<<<<<<<...<
<<<<<.......>>>>>>.....<<...((>>....>>>>>>>...<<<.
.<<<<....>>>>..>>>.<<<....>>>...)).((((((..((((.>>
>>>>))))))))))...]]]]..}}]]]]]........<<<<<<...<<.
......>>...>>>>>>...............<<<<...<<<<..<.<<<
<<<<......>>>>>>>...>.<<<.........>>>.....>>>>....
...>>>>...<<<<<<<....>>>.>>>>........}}}.]]]]]....
<<<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<
<...<<<<....<<<<<<<<<<}}}.>>>>>..>>>>>...>>>>..>>>
>>.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>>>
>>>>>..<........<.......<<..........<<<<<<.....>>>
>>>......>>.......<<<<<<...<<<<<.<..<<<......>>>..
>.>>>>>.>>>>>>..............>...........><<<<<.<..
...........>.....<<<<<<<<<.<<<....<<<<....<<<<<.>>
>>>...>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6ylh Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): V18 N20 S22 G24 S48 A50 R51 Y52 F57 H68 R94 N95 T99 N101 G103 F104 G105 G124 D126 Y128 K142
• Binding residue (residue number reindexed from 1): V13 N15 S17 G19 S43 A45 R46 Y47 F52 H63 R89 N90 T94 N96 G98 F99 G100 G119 D121 Y123 K137

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6ylh, PDBe:6ylh, PDBj:6ylh
• PDBsum: 6ylh
• PubMed: 32668200
• UniProt: P0C0W9|RL11A_YEAST Large ribosomal subunit protein uL5A (Gene Name=RPL11A)