Structure of PDB 5jea Chain J Binding Site BS01

Receptor Information
>5jea Chain J (length=936) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
RRKRLADGLSVTQKVFVRSRNGGATKIVREHYLRSDIPCLSRSCTKCPQI
VVPDAQNELPKFILSDSPLELSAPIGKHYVVLDTNVVLQAIDLLENPNCF
FDVIVPQIVLDEVRNKSYPVYTRLRTLCRDSDDHKRFIVFHNEFSEHTFV
ERLPNETINDRNNRAIRKTCQWYSEHLKPYDINVVLVTNDRLNREAANII
TKSLVQYIELLPNADDIRDSIPQDFTFPEYYSTARVMGGLKNGVLYQGNI
QISEYNFLEGSVSLPRFSKPVLIVGQKNLNRAFNGDQVIVELLPQSEWKA
PSSIVLDSEHFDISDKQRRLLAKDAMIAQRSKKIQPTAKVVYIQRRSWRQ
YVGQLAPSSVDPQSSSTQNVFVILMDKCLPKVRIRTRRAAELLDKRIVIS
IDSWPTTHKYPLGHFVRDLGTIESAQAETEALLLEHDVEYRPFSKKVLEC
LPAEGHDWKAPTKLDDPEAVSKDPLLTKRKDLRDKLICSIDPPGCVDIND
ALHAKKLPNGNWEVGVHIADVTHFVKPGTALDAEGAARGTSVYLVDKRID
MLPMLLGTDLCSLKPYVDRFAFSVIWELDDSANIVNVNFMKSVIRSREAF
SYEQAQLRIDDKTQNDELTMGMRALLKLSVKLKQKRLEAGALNLASPEVK
VHMDSEEVEIKKLLATNSLVEEFMLLANISVARKIYDAFPQTAMLRRHAA
PPSTNFEILNEMLNTRKNMSISLESSKALADSLDRCVDPEDPYFNTLVRI
MSTRCMMAAQYFYSGAYSYPDFRHYGLAVDIYTHFTSPIRRYCDVVAHRQ
LAGAIGYEPLSLTHRDKNKMDMICRNINRKHRNAQFAGRASIEYYVGQVM
RNNESTETGYVIKVFNNGIVVLVPKFGVEGLIRLDNLTEDPNSAAFDEVE
YKLTFVPTNSDKPRDVYVFDKVEVQVRSVMDELLLK
Ligand information
>5jea Chain R (length=27) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cccccgaaggggguuuuuuuuuuuuua
<<<<....>>>>...............
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5jea Structure of a Cytoplasmic 11-Subunit RNA Exosome Complex.
Resolution2.65 Å
Binding residue
(original residue number in PDB)		K22 F24 R37 D543 P544 G546 C547 V548 D549 N551 D552 Y595 K599 R600 Y654 L696 E700 E728 M731 L732 R753 H755 R811 A815 A816 H831 Y839 H841 T843 R847 R889 R896
Binding residue
(residue number reindexed from 1)		K14 F16 R29 D491 P492 G494 C495 V496 D497 N499 D500 Y543 K547 R548 Y602 L644 E648 E671 M674 L675 R696 H698 R754 A758 A759 H774 Y782 H784 T786 R790 R832 R839
Enzymatic activity
Catalytic site (original residue number in PDB) D543 D549 N551 D552 Y654 R847
Catalytic site (residue number reindexed from 1) D491 D497 N499 D500 Y602 R790
Enzyme Commision number 3.1.13.-
3.1.26.-
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000175 3'-5'-RNA exonuclease activity
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004521 RNA endonuclease activity
GO:0004527 exonuclease activity
GO:0004540 RNA nuclease activity
GO:0005515 protein binding
Biological Process
GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0006364 rRNA processing
GO:0006396 RNA processing
GO:0006397 mRNA processing
GO:0006401 RNA catabolic process
GO:0016075 rRNA catabolic process
GO:0043628 regulatory ncRNA 3'-end processing
GO:0070651 nonfunctional rRNA decay
GO:0071028 nuclear mRNA surveillance
GO:0071031 nuclear mRNA surveillance of mRNA 3'-end processing
GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process
GO:0071038 TRAMP-dependent tRNA surveillance pathway
GO:0071039 nuclear polyadenylation-dependent CUT catabolic process
Cellular Component
GO:0000176 nuclear exosome (RNase complex)
GO:0000177 cytoplasmic exosome (RNase complex)
GO:0000178 exosome (RNase complex)
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005739 mitochondrion

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5jea, PDBe:5jea, PDBj:5jea
PDBsum5jea
PubMed27345150
UniProtQ08162|RRP44_YEAST Exosome complex exonuclease DIS3 (Gene Name=DIS3)

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