Structure of PDB 3j8g Chain J Binding Site BS01

Receptor Information
>3j8g Chain J (length=142) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDT
GDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERV
IEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI
Ligand information
>3j8g Chain B (length=2874) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaa
aaauuagcggaugacuuguggcugggggugaaaggccaaucaaaccggga
gauagcugguucuccccgaaagcuauuuagguagcgccucgugaauucau
cuccggggguagagcacuguuucggcaagggggucaucccgacuuaccaa
cccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacgg
cgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcua
aggucccaaagucaugguuaagugggaaacgaugugggaaggcccagaca
gccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagc
ucacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugc
accgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguuc
uguaagccugcgaaggugugcugugaggcaugcuggagguaucagaagug
cgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgg
aagaccaaggguuccuguccaacguuaaucggggcagggugagucgaccc
cuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccu
guacuugguguuacugcgaaggggggacggagaaggcuauguuggccggg
cgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccg
gaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaac
aaaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaauc
guaccccaaaccgacacagguggucagguagagaauaccaaggcgcuuga
gagaacucgggugaaggaacuaggcaaaauggugccguaacuucgggaga
aggcacgcugauauguaggugaggucccucgcggauggagcugaaaucag
ucgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugc
aaacacgaaaguggacguauacggugugacgccugcccggugccggaagg
uuaauaucgaagccccgguaaacggcggccguaacuauaacgguccuaag
guagcgaaauuccuugucggguaaguuccgaccugcacgaauggcguaau
gauggccaggcugucuccacccgagacucagugaaauugaacucgcugug
aagaugcaguguacccgcggcaagacggaaagaccccgugaaccuuuacu
auagcuugacacugaacauugagccuugauguguaggauaggugggaggc
uuugaaguguggacgccagucugcauggagccgaccuugaaauaccaccc
uuuaauguuugauguucuaacguugacccguaauccggguugcggacagu
gucugguggguaguuugacuggggcggucuccuccuaaagaguaacggag
gagcacgaagguuggcuaauccuggucggacaucaggagguuagugcaau
ggcauaagccagcuugacugcgagcgugacggcgcgagcaggugcgaaag
caggucauagugauccggugguucugaauggaagggccaucgcucaacgg
auaaaagguacuccggggauaacaggcugauaccgcccaagaguucauau
cgacggcgguguuuggcaccucgaugucggcucaucacauccuggggcug
aaguaggucccaaggguauggcuguucgccauuuaaagugguacgcgagc
uggguuuagaacgucgugagacaguucggucccuaucugccgugggcgcu
ggagaacugaggggggcugcuccuaguacgagaggaccggaguggacgca
ucacugguguucggguugucaugccaauggcacugcccgguagcuaaaug
cggaagagauaagugcugaaagcaucuaagcacgaaacuugccccgagau
gaguucucccugacccuuuaaggguccugaaggaacguugaagacgacga
cguugauaggccggguguguaagcgcagcgaugcguugagcuaaccggua
cuaaugaaccgugaggcuuaaccu
<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<......
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<........>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<......>
>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<.<.<<<<<<.....<<<<<<<<<..<
<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<......
..>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>.
..>..>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<
<<<<<<.....<<<...<<<.<<<<...<<.<<<<.....>>>>..>>..
.>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<
<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<..
.<<<(((.....<<<<<<...........<<..<<<<<..<<........
<<<<<.<<...<<<<.......>>>>...>>........<<<......>>
>..>>>>>....>>..>>>>>.>>...........))).....>>>>>>.
>>>...>>>>>>><<<<<<<<<....>>>>>>>>>....>>>>>><<<<<
<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>.>>
.....>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<..
..<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<
<...<<<...<<<....>>>...>>>...>>>..<<<<<........>>>
>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<
....(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>
>>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>
>...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<
<....<<<<<<<<.<<...............<<<<<....<....>....
.>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>
>............<<<<<<<<<..<<...........>>..<<<<<<<..
...<<<....>>>.......>>>>>>>.<<<<<......<<<<<<...<<
<<........>>>>.>>>>..>>....<<<<<.......>>>>>......
...............<<<<<.......>>>>>..........>>>>>..>
>.>>>>>>>.....>>.>>>>>>>>.>>>>>>...>.>.....<<<<<..
......>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<
<..<<<.<<<<<.<<<<<<<<.<<.<.<......................
..................................................
.>..>.>>.>>>>>>>>...<<<.<<<<<<.....>>>>>>>>>....>>
>>>.>>>...>>>>..<<<<.....>>>><<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...((
(>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>
>>.>>))).......<<<<<<<.((..>>>>..))>>>>>>>........
.....>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>
>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.
.....>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....
<<<<<<<<..<<<<..<<<<<<<.....<....>....>>>>>>>.<<<<
<...<<<<...<<<<<<<<<<<))).>>>>>>..>>>>>...>>>>.>>>
>>...<<<....<<<<<...........>>>>>....>>>.>>>>>....
..>>>>>>>..<<<<<<...>>>>>>.....<<..<<<<.........>>
>>....<<<<<<<<..<<....<<<<<....>>>>>...>>...>>>>>.
>>>.....>>.....>>>>>>>>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB3j8g Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly
Resolution5.0 Å
Binding residue
(original residue number in PDB)		M1 T3 K7 L25 R27 T30 R34 R37 T65 K68 K72 Y75 H76 H77 H80 I81 G82 Q86 E91 R95 R96 E102 I103 K106 G107 M108 P110 P113 L114 R120 A134 Q136
Binding residue
(residue number reindexed from 1)		M1 T3 K7 L25 R27 T30 R34 R37 T65 K68 K72 Y75 H76 H77 H80 I81 G82 Q86 E91 R95 R96 E102 I103 K106 G107 M108 P110 P113 L114 R120 A134 Q136
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003729 mRNA binding
GO:0003735 structural constituent of ribosome
GO:0008270 zinc ion binding
GO:0048027 mRNA 5'-UTR binding
GO:0070180 large ribosomal subunit rRNA binding
Biological Process
GO:0002181 cytoplasmic translation
GO:0006412 translation
GO:0017148 negative regulation of translation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3j8g, PDBe:3j8g, PDBj:3j8g
PDBsum3j8g
PubMed25389271
UniProtP0AA10|RL13_ECOLI Large ribosomal subunit protein uL13 (Gene Name=rplM)

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