Structure of PDB 8inf Chain I Binding Site BS01

Receptor Information

>8inf Chain I (length=190) Species: 9606 (Homo sapiens)

MKTILSNQTVDIPENVDITLKGRTVIVKGPRGTLRRDFNHINVELSLLGK
KKKRLRVDKWWGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYAHFP
INVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELILEGND
IELVSNSAALIQQATTVKNKDIRKFLDGIYVSEKGTVQQA

Ligand information

>8inf Chain 2 (length=3654)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcggguccggccgugucggcgg
cccggcggaucuuucccgcgcgggggaccgucccccgaccggcgaccggc
cgccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccggg
acggcugggaaggcccggcggggaagguggcucgggggcccccgaguguu
acagcccccccggcagcagcacucgccgaaucccggggccgagggagcga
gacccgucgccgcgcucuccccccucccggcgcgccggggggggccgggc
caccccucccacggcgcgaccgcucggggcggacuguccccagugcgccc
cgggcgggucgcgccgucgggcccgggggaggccacgcgcgcgucccccg
aagagggggacggcggagcgagcgcacggggucggcggcgacgucggcua
cccacccgacccgucuugaaacacggaccaaggagucuaacacgugcgcg
agucgggggcucgcacgaaagccgccguggcgcaaugaaggugaaggccg
gcgcgcucgccggccgaggugggaucccgaggccucuccaguccgccgag
ggcgcaccaccggcccgucucgcccgccgcgccggggagguggagcacga
gcgcacguguuaggacccgaaagauggugaacuaugccugggcagggcga
agccagaggaaacucugguggagguccguagcgguccugacgugcaaauc
ggucguccgaccuggguauaggggcgaaagacuaaucgaaccaucuagua
gcugguucccuccgaaguuucccucaggauagcuggcgcucucgcagacc
cgacgcccgccacgcaguuuuauccgguaaagccuuuaaauggguaagaa
gcccggcucgcuggcguggagccgggcguggaaugcgagugccuaguggg
ccacuuuugguaagcagaacuggcgcugcgggaugaaccgaacgccgggu
uaaggcgcccgaugccgacgcucaucagaccccagaaaagguguugguug
auauagacagcaggacgguggccauggaagucggaauccgcuaaggagug
uguaacaacucaccugccgaaucaacuagcccugaaaauggauggcgcug
gagcgucgggcccauacccggccgucgccggcagucgagaguggacggga
gcggcggcccggagccccgcggacgcuacgccgcgacgaguaggagggcc
gcugcggugagccuugaagccuagggcgcgggcccggguggagccgccgc
aggugcagaucuuggugguaguagcaaauauucaaacgagaacuuugaag
gccgaaguggagaaggguuccaugugaacagcaguugaacaugggucagu
cgguccugagagaugggcgagcgccguuccgaagggacgggcgauggccu
gcccuccgaucgaaagggagucggguucagauccccgaauccggaguggc
ggagaugggcgccgcgaggcguccagugcgguaacgcgaccgaucccgga
gaagccggcgggagccccggggagaguucucuuuucuuugugaagggcag
ggcgcccuggaauggguucgccccgagagaggggcccgugccuuggaaag
cgucgcgguuccggcggcguccggugagcucucgcuggcccuugaaaauc
cgggggagaggguguaaaucucgcgccgggccguacccauauccgcagca
ggucuccaaggugaacagccucuggcauguuggaacaauguagguaaggg
aagucggcaagccggauccguaacuucgggauaaggauuggcucuaaggg
cugggucggucgcggccggcgccuagcagccgacuuagaacuggugcgga
ccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcg
gcggguguugacgcgaugugauuucugcccagugcucugaaugucaaagu
gaagaaauucaaugaagcgcggguaaacggcgggaguacucucuuaagaa
cgagauucccacugucccuaccuacuauccagcgaaaccacagccaaggg
aacgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacu
cuagucuggcacggugaagagacaugagagguguagaauaagugggaggc
ccccggcgcccccccgguguccccgcgaggggcccggggcgggguccgcc
ggcccugcgggccgccggugaaauaccacuacucugaucguuuuuucacu
gacccggugaggcgggggggcgagccccgaggggcucucgcuucuggcgc
caagcgcccggccgcgcgccggccgggcgcgacccgcuccggggacagug
ccagguggggaguuugacuggggcgguacaccugucaaacgguaacgcag
guguccuaaggcgagcucagggaggacagaaaccucccguggagcagaag
ggcaaaagcucgcuugaucuugauuuucaguacgaauacagaccgugaaa
gcggggccucacgauccuucugaccuuuuggguuuuaagcaggagguguc
agaaaaguuaccacagggauauuguggcggccaagcguucauagcgacgu
cgcuuuuugcuucgaugucggcucuuccuaucauugugaagcagaauuca
ccaagcguuggauuguucacccacuaauagggaacgugagcuggguuuag
accgucgugagacagguuaguuuuacccuacugaugauguguuguugcca
ugguaauccugcucaguacgagaggaaccgcagguucagacauuuggugu
augugcuuggcugaggagccaauggggcgaagcuaccaucugugggauua
ugacugaacgccucuaagucagaaucccgcccaggcggaacgauacggca
gcgccgcggagccucgguuggccucggauagccggucccccgccgggguc
cggugcggagugcccuucguccugggaaacggggcgcggccggagaggcg
gccgcccccucgcccgucacgcaccgcacguucguggggaaccuggcgcu
aaaccauucguagacgaccugcuucugggucgggguuucguacguagcag
agcagcucccucgcugcgaucuauugaaagucagcccucgacacaagggu
uugu

.........................<<<<<<.....<<....>>......
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..>>>>>..<<<<<<<<<<...<<.<<<<<........<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>...>>>>>.>>..>>>>>>>>>>.......<<<<<<<........>>>
>>>>.....<<<<<..((((((>>>>>..>>>..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<.<<<.....<<<<<<<<<<<..<<<<<<<.......<<
....<<<<.<<<..<<.<..>.>>>>>>>>>>>......>>>>...>>>>
>>>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<
<<<<<...<<..<<<....<<<<......<<<<<<<<..>>>>>>>>...
....>>>>...>>>........>>>>>>..>>>>>>>>>>>.........
...........<<<<<..<<<<<<<............>>>>>>>.<<<<<
<.<<<<.<<<<<<<<<<.<<<<<..<<<<<.<..<......>.>..>>>>
>..>>>>.>.>>>>>>>.>>>...>>>>..>>>>.>>..<<<<<<<<<..
.....>>>>>>>.>>.....>>>>>......>>.>>>>>...>>>>>...
>>>>>>>>..<<<<........>>>>.<<<<<....<<<<<<<<<<<<<.
.<<<...<<<<........>>>>.....>>>...<<....>>...<<<<<
<<......>>>>>>>..<<<<<...<<<..<<<..[[....(((>>>..>
>>....>>>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>....
>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>
>>.>>>....>>>>>>>>>...<<.......>>......>>>>>>...>.
.>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<.........
.............<<<.<<<<<<<<....<<<..>>>....>>>>>>...
<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<
<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<
...<<<(((.......<<<<<.....<<..........<<<.<<<<<<<<
<........<<<<<.<<....<<.........>>....>>.........<
<......>>...>>>>>....>>>>>>>>>>>>..{.......))).>>.
..>>>>>..>>>...>>>>>>><<<<<<.<<<..........)))....]
]............................>>>>>>>>>....>>>>>>.<
<<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>
>>.>>.....>>>>>.<<<....<<.....<<<<<<.......>>>>>>.
<<<.....<<<<<.<<...<<<<<....<<.....>>..>>>>>....((
<<<<<<...<<....<<<....>>>.............<<<<........
>>>>.....>>....>>>..<<<<<........>>>>>))<<<<....<<
<<<...<<<<<<<....>>>>>>>...<<<....>>>...<..<<<<<<<
...<<<<<<<<<<<<<<<<<<<<<<<..[[[..>>>>>>....<<<<<.<
<<<.<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>.....<
<<<<<<.......>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>
>>>>>..>..........>>.>>>.>>>>.>>>..>>.....<<......
>>>>>>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<.
..............<<<<<....<<..>>.....>>>>><<.<<<<<<<<
<<<.......................>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>.......<<<..<<<<<<....<<<....<<
<<<....>>>>.>...>>>.>>>>..>>>>><<<.<...>.>>>......
>>.>>>>>>>.....>>.>>>>>>>..>>>>>>...>>>....<<<<<..
......>>>>>....<<<<<..<<<........<<<<<.<<<<<<..<<<
<<.<<<<<<<<<.<<<<<<<<.<<<.........................
..................................................
....................................>>.>.>>>>>>>>.
..<<<<<.....<<<<........<<....>>..................
...<<<<<<<<<<.......>>>>>>>>>>..>>>>..>>>>>....>>>
>>>>>>..>>>>>...<<<.....>>>.<<<<<<<...........>>>>
>>>.<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.((
(>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....
>>>.>>)))].....<<<<<<<..<<<..)))))).>>>>>>>>>>....
.....>>>>..>>>>>>>....}..<<<<<<...<<.......>>...>>
>>>>.........((...<<<<.<<<<<<..<.<.<<<<<......>>>>
>...>.>.<<<.....))..>>>.....>>>>>..>...>>>>......<
<<<<<<....>>>.>>>>........>>>.>>>>>.....<<<<<<.<..
<<<..<.<<<<<.................>>>>>.><<<<....<<<<..
..<<.<<<<<<.]]]..>>>>..>>.>>...>>>>...>>>><<<<<...
<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>.....<
<<<<<..<<..<<<<....<<<........<<<<<.<<<<....>>>>.>
>>>>.......>>>...<<<..<<<<....<<<<.<<<<<<<......>>
>>>>>>>>>...>>>>..>>>..............>>>>..>>.>>>>>>
<<<<<.<.............>....<<.<<<<<<<.<<.....<<<<.<.
.<<<<<......>>>>>..>>>>>....>>....>>>>.>>>.>>..>>>
>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8inf Visualizing the nucleoplasmic maturation of human pre-60S ribosomal particles.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): R23 H40 N42 V43 E44 K50 K52 K59 W60 R64 K65 A68 R71 T72 S75 H76 N79 H98 F99 K121 E152 S155 Q163 K168 K170 D171 R173 K174 F175
• Binding residue (residue number reindexed from 1): R23 H40 N42 V43 E44 K50 K52 K59 W60 R64 K65 A68 R71 T72 S75 H76 N79 H98 F99 K121 E152 S155 Q163 K168 K170 D171 R173 K174 F175

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8inf, PDBe:8inf, PDBj:8inf
• PDBsum: 8inf
• PubMed: 37491604
• UniProt: P32969|RL9_HUMAN Large ribosomal subunit protein uL6 (Gene Name=RPL9)