Structure of PDB 8a57 Chain I Binding Site BS01

Receptor Information

>8a57 Chain I (length=203) Species: 169963 (Listeria monocytogenes EGD-e)

LSLLKQDGTNAGEITLNDTVFGIEPNEKVVVDVILSQRASLRQGTHKVKN
RSEVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLPK
KVRRLAIKSILSSKVNEEKLVVLEGLTFDAPKTKEFAAFLKNISVDTKAL
IVVAGESENVELSARNLQGITVIPAESISVLEVAKHDKLIITKAAVEKVE
EVL

Ligand information

>8a57 Chain A (length=2908)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaaga
aggacgggacuaacaccgauaugcuuuggggagcuguacguaagcguuga
uccagagauuuccgaaugggggaacccacuaucuuuagucggauaguauc
cuuacgugaauacauagcgugaggaaggcagacccagggaacugaaacau
cuaaguaccuggaggaagagaaagaaaaaucgauuuccugaguagcggcg
agcgaaacggaaagagcccaaaccaagaagcuugcuucuugggguuguag
gacacucuauauggaguuacaaaagaaaguuauaaaugaagcggucugga
aaggcccgccaaagacgguaacagcccgguaguugaaauggcuuucccuc
cagaguggauccugaguacggcggaacacgugaaauuccgucggaauccg
ggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaacagu
uccugaaaccgugugccuacaaguaguuagagcccguuaaugggugauag
cgugccuuuuguagaaugaaccggcgaguuacgauuuguugcaagguuaa
gcggaaaaagcggagccguagcgaaagcgagucugaauagggcgcauaag
uaacaggucguagacccgaaaccaggugaucuacccauguccaggaugaa
gguaagguaauacuuacuggagguccgaacccacgcacguugaaaagugc
ggggaugagguguggguagcggagaaauuccaaucgaacuuggagauagc
ugguucucuccgaaauagcuuuagggcuagccucgagguaaagagucaug
gagguagagcacuguuuggacuaggggcccuucucggguuaccgaauuca
gauaaacuccgaaugccauguacuuauacucgggagucagacugcgagug
auaagauccguagucgaaagggaaacagcccagaccaccaguuaaggucc
ccaaauauauguuaaguggaaaaggaugugggguugcuuagacaaccagg
auguuggcuuagaagcagccaccauugaaagagugcguaauagcucacug
gucgagugaccccgcgccgaaaauguaccggggcuaaacauauuaccgaa
acuguggaugaaccgguucgugguaggagagcguucuaagggcggugaag
ucagaccggaaggacugguggagcgcuuagaagugagaaugccgguauga
guagcgaaagaagggugagaaucccuuccaccgaauaucuaagguuuccu
gaggaaggcucguccgcucaggguuagucgggaccuaagccgaggccgau
aggcguaggcgauggacaacagguagagauuccuguaccagugcuaauug
uuuaaccgauggggugacacagaaggauagggaaucgcacgaauggaaau
gugcguccaagcagugagugugagaaguaggcaaauccgcuucucgcgaa
gcaugagcugugauggggaacggaaguuccugauuucacgcugucaagaa
aagccucuaggaagaguaguacugcccguaccgcaaaccgacacagguag
augaggagagaauccuaaggugagcgagagaacucucguuaaggaacucg
gcaaaaugaccccguaacuucgggagaaggggugcucuauuagggugcaa
gcccgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaaca
caggucucugcaaaaccguaaggugacguauaggggcugacgccugcccg
gugcuggaagguuaagaggagugcuuagcuucggcgaagguacgaauuga
agccccaguaaacggcggccguaacuauaacgguccuaagguagcgaaau
uccuugucggguaaguuccgacccgcacgaaaggcgcaacgaucugggca
cugucucaacgagagacucggugaaauuauaguaccugugaagaugcagg
uuacccgcgacaggacggaaagaccccguggagcuuuacugcaaccugau
auggaauguuuguaccgcuuguacaggauagguaggagccgaagagacgu
gugcgcuagcauacgaggaggcaauggugggauacuacccuggcuguaug
accauucuaacccgccacgcuuagcgcguggggagacagugucagguggg
caguuugacuggggcggucgccuccuaaagaguaacggaggcgcccaaag
guucccucagaauggauggaaaucauucgcagaguguaaaggcacaaggg
agcuugacugcgagacugacaagucgagcagggacgaaagucgggcuuag
ugauccggugguuccgcauggaagggccaucgcucaacggauaaaagcua
ccccggggauaacaggcuuaucucccccaagaguccacaucgacggggag
guuuggcaccucgaugucggcucgucgcauccuggggcuguagucggucc
caaggguugggcuguucgcccauuaaagcggcacgcgagcuggguucaga
acgucgugagacaguucggucccuauccgucgcgggcgcaggaaauuuga
gaggagcuguccuuaguacgagaggaccgggauggacacaccgcuggugu
accaguuguuccgccaggagcaucgcuggguagcuauguguggcagggau
aaacgcugaaagcaucuaagcgugaagccccccucaagaugagauuuccc
auuucuucggaaaguaagaucccugaaagaugaucagguagauagguuug
gaguggaaguguagcgauacauggagcggacaaauacuaaucgaucgagg
acuuaacc

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>....<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<
<<<<<<<<....<<<<........<<<<<<<<<.<<<.....<<<<....
..>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>.>>>
>>>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]...........
....<<....>>....]].........<<<<....>>>>...........
.......>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...
<<.......>>...>>>...<<....>>..<<<<.....>>>>......>
>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....
<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>
>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>
.>>..<<<<<<....<<<<<....>>>>><<<<<<<<.<...<<<<<<<<
<.....<<<...<<<<<<<<...<<.<<<<.....>>>>..>>...>>>>
>>>>..>>.>.....>>>>>.>>>>..>>>>>>>.>>..<<<<<<<.<<.
.....>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<((
(.....<<<<<<...........<<..<<<<<<<<<........<<<<<.
<<...<<<<.......>>>>...>>.........<<......>>...>>>
>>....>>>>>>>>>.>>...........))).....>>>>>>.>>>...
>>>>>>><<<<<<..>>>>>>....>>>>>>..<<<<<<<.<<......<
<<<<.<<....>>.>>>>>...>>.>>>>>>>........>>>>>><...
...<<....<<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<
<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<...
.>>>...>>>...>>...<<<<<........>>>>>))<<<<<<<<.<<.
.....<<...<<<<<...<<<<..<<<..<<<<<.<<<<<...(((....
>>>>>.<<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>.....
>>>>..>>>>>...>>..........>>>>>...>>>...>>>>......
..>>>>>..>>..>.>>>>>>>>>>>>>.>>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.......
..<.....<<<<<...............>>>>><<<<<....<<<<....
>>>>...>>>>>............<<<<<<<<<..<<<.........>>>
..<<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..
<<<<<<<..<<<<..<<...<<<<<..<<....>>...>>>>>...>>..
>>>>>>>>>..>>>>><<<<<<.......>>>>>>........<......
.....<<<<<<.....>>>>>>.....>....>>>>...>>.>>>>>>>.
.>..>>.>>>>>>>>.>>>>>>.....>.(...<<<<<.......)>>>>
>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<
<<.<<<<<.<<<......................<...<...........
.<<<....>>>.........>.............>.............>>
>.>>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>
>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<...<
<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>....>>>
>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>)))].
.....<<<<<<<.((..>>>>..))>>>>>>>.............>>>>.
.]]>>>>.......<<<<...<<<<<<...<<.......>>...>>>>>>
....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>
>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<
<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...
<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<<<..
.<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<..
..<<<<<...........>>>>>....>>>.>>>>.......>>>>>>>.
.<<<<....>>>>....<<<<<<<<<.......>>>>>....<<<<<<<<
..<<....<<<<<....>>>>>...>>...>>>>>.>>>...>>>>....
>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8a57 Structural basis for HflXr-mediated antibiotic resistance in Listeria monocytogenes.
• Resolution: 2.3 Å
• Binding residue (original residue number in PDB): V32 R41 R45 Q46 T48 H49 K50 V51 K52 N53 R54 S55 V57 R58 G59 R62 K63 Q67 K68 G69 G71 R72 A73 R74 Q75 G76 R79 S80 P81 Q82 W83 R84 V88 V89 F90 G91 P92 R95 Y99 K100 P102 K103 K104 V105 R106 R107 P134 K135 T136 K137 K151 L165 R168 N169 Q171 E179 S180 S182
• Binding residue (residue number reindexed from 1): V29 R38 R42 Q43 T45 H46 K47 V48 K49 N50 R51 S52 V54 R55 G56 R59 K60 Q64 K65 G66 G68 R69 A70 R71 Q72 G73 R76 S77 P78 Q79 W80 R81 V85 V86 F87 G88 P89 R92 Y96 K97 P99 K100 K101 V102 R103 R104 P131 K132 T133 K134 K148 L162 R165 N166 Q168 E176 S177 S179

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8a57, PDBe:8a57, PDBj:8a57
• PDBsum: 8a57
• PubMed: 36300626
• UniProt: P61055|RL4_LISMO Large ribosomal subunit protein uL4 (Gene Name=rplD)