Structure of PDB 7wtw Chain I Binding Site BS01

Receptor Information

>7wtw Chain I (length=205) Species: 9606 (Homo sapiens)

GISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGG
NKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCI
VLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDE
RKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRK
IKARK

Ligand information

>7wtw Chain 2 (length=1586)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
ccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggua
gucgccgugccuaccauggugaccacgggugacggggaaucaggguucga
uuccggagagggagccugagaaacggcuaccacauccaaggaaggcagca
ggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaauaa
caauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaaa
uccuuuaacgaggauccauuggagggcaagucuggugccagcagccgcgg
uaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcuc
guaguuggaucucucucggccgaagcguuuacuuugaaaaaauuagagug
uucaaagcaggccgccuggauaccgcagcuaggaauaauggaauaggacc
gcgguucuauuuuguugguuuucggaacugaggccaugauuaagagggac
ggccgggggcauucguauugcgccgcuagaggugaaauucuuggaccggc
gcaagacggaccagagcgaaagcauuugccaagaauguuuucauuaauca
agaacgaaagucggagguucgaagacgauaccgucguaguuccgaccaua
aacgaugccgaccggcgaugcggcggcguuauucccaugacccgccgggc
agcuuccgggaaaccaaagucuuuggguuccggggggaguaugguugcaa
agcugaaacuuaaaggaauugacggaagggcaccaccaggaguggagccu
gcggcuuaauuugcacccggcccgacacggacaggauugacagauugaua
gcucuuucucgauuccgugggugguggugcauggccguuguuggcugguu
aauuccgaugaacgagacucuggcaugcuauucuuagagggacaaguggc
guucagccacccgagauugagcuaacaggucugugaugcccuguccgggg
cugcacgcgcgcuacacugacuggcucagcguguccuacgccggcaggcg
cggguaacccguugaaccccauucgugauggggaucggggauugcaauua
uuccccaugaacgaggaauucccaguaagugcgggucauaagcuugcguu
gauuaagucccugcccuuuguacacaccgcccgucgcuacuaccgauugg
augguuuagugaggcccucggaucggccccgccggggugcccuggcggag
cgcugagaagacggucgaacuugacuaucuagaggaaguaaaagucgagg
uuuccguaggugaaccugcggaaggaucauuaacgg

...<<<<<.[[((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<<.........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<<....>>>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<..>>>>>>>.>>.>>>...>>..>.>>>.<<<....<<<....<<<<<<
<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>..
..>>>.>>.<<.<<<..........>>>.>>.<.<<....>>.>...>>>
>>>.........<<<....<<<<...>>>>..>>>..>...>>.>.....
<<<<<<<<<.<<<....>>>..>>>>>>.>>>......<<..<.......
....>..>>.........<<<<<((......<<<<.....<<..))>>..
.....>>>>.>>>>>..>>>>>>.>>>.........<.<((.....<.<<
...<<<.<<...<<.<<<..>>>>>...<<<<<<.<.......<<...<.
......>.<<<<.>>>>...>>......>.>>>..>>>........<.<<
.<<<<<<<...............>>>>>>>.>>.>....>>....<<<<<
<..<...<<<<..<<<.<<<<<<<<...<<<......>>>......>>>>
>>>>.>>>.......<<....>>...>>>>..>..>>>.>>>...>>>..
.>>.>....<<<<<<<...<...<<<<....>>>>...>>>>>>>>....
......<<<.<<.<<<..<.<<<<<<.<<<........>>>>>>>>>.>.
.>>>...<<..))>>...>>.....>>>.>.>.<<<......<<<<....
>>>>....>>>..)))).]]....<<<<<<<<..<<..<<<<<.<<<<.<
<<<<<..........<<<<<.....<<<<<<.......<<.<<<......
..>>>.>>.....>>>>>>......<<<.<<.<<<<<<.<<<...<<<..
....>>>...>>>....<<<...<...<<<....>>>..>.....<<<<<
.....>>>>>........>>>...>>.>>>>..>>..>>>....>>>>>.
..>>.>>>>.>>>.>...<<.<<<........<.<<<.<<<<....>>>>
.>>>....>.........>>>.>>......<.....<<<<<.........
.>>>>>.....>.....>>>>>.....<<<<<<<<<.....>>>>>>>>>
......>>...>>>>>>>>......<<<...<<....<<<<.<<....<<
<<<<<<.<<<..<<<<.<<...<<<<<<<<<<<<<<<..>>>>>>>>>.>
.>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.>>>>.....>>.>>
><<<<<<<<<....>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wtw The nucleoplasmic phase of pre-40S formation prior to nuclear export.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): G2 I3 R5 D6 N7 H9 K10 R11 R12 K13 T14 G16 K19 Y21 K23 K24 R25 K26 Y27 L29 G30 R31 P32 A33 A34 R41 R42 I43 H44 T45 R47 R49 G50 N52 K53 K54 Y55 R56 L58 N64 C71 T73 K75 S86 N87 K98 N99 K144 I145 K148 R178 Q181 R184 D186
• Binding residue (residue number reindexed from 1): G1 I2 R4 D5 N6 H8 K9 R10 R11 K12 T13 G15 K18 Y20 K22 K23 R24 K25 Y26 L28 G29 R30 P31 A32 A33 R40 R41 I42 H43 T44 R46 R48 G49 N51 K52 K53 Y54 R55 L57 N63 C70 T72 K74 S85 N86 K97 N98 K143 I144 K147 R177 Q180 R183 D185

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7wtw, PDBe:7wtw, PDBj:7wtw
• PDBsum: 7wtw
• PubMed: 36321656
• UniProt: P62241|RS8_HUMAN Small ribosomal subunit protein eS8 (Gene Name=RPS8)