Structure of PDB 7wtt Chain I Binding Site BS01

Receptor Information

>7wtt Chain I (length=205) Species: 9606 (Homo sapiens)

GISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGG
NKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCI
VLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDE
RKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRK
IKARK

Ligand information

>7wtt Chain 2 (length=1575)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
ccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggua
gucgccgugccuaccauggugaccacgggugacggggaaucaggguucga
uuccggagagggagccugagaaacggcuaccacauccaaggaaggcagca
ggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaauaa
caauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaaa
uccuuuaacgaggauccauuggagggcaagucuggugccaauuccagcuc
caauagcguauauuaaaguugcugcaguuaaaaagcucguaguuggaucu
cucucggccgaagcguuuacuuugaaaaaauuagaguguucaaagcaggc
cgccuggauaccgcagcuaggaauaauggaauaggaccgcgguucuauuu
uguugguuuucggaacugaggccaugauuaagagggacggccgggggcau
ucguauugcgccgcuagaggugaaauucuuggaccggcgcaagacggacc
agagcgaaagcauuugccaagaauguuuucauuaaucaagaacgaaaguc
ggagguucgaagacgauaccgucguaguuccgaccauaaacgaugccgac
cggcgaugcggcggcguuauucccaugacccgccgggcagcuuccgggaa
accaaagucuuuggguuccggggggaguaugguugcaaagcugaaacuua
aaggaauugacggaagggcaccaccaggaguggagccugcggcuuaauuu
gcacccggcccggacacggacaggauugacagauugauagcucuuucucg
auuccgugggugguggugcauggccguuguuggcugguuaauuccgauga
acgagacucuggcaugcuauucuuagagggacaaguggcguucagccacc
cgagauugagcuaacaggucugugaugcccuguccggggcugcacgcgcg
cuacacugacuggcucagcguguccuacgccggcaggcgcggguaacccg
uugaaccccauucgugauggggaucggggauugcaauuauuccccaugaa
cgaggaauucccaguaagugcgggucauaagcuugcguugauuaaguccc
ugcccuuuguacacaccgcccgucgcuacuaccgauuggaugguuuagug
aggcccucggaucggccccgccggggugcccuggcggagcgcugagaaga
cggucgaacuugacuaucuagaggaaguaaaagucgagguuuccguaggu
gaaccugcggaaggaucauuaacgg

...<<<<<.[[((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<..........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<<....>>>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<..>>>>>>>..>.>>>...>>..>.>>>.<<<....<<<....<<<<<<
<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>..
..>>>.>>.<<.<<<..........>>>.>>.<.<<....>>.>...>>>
>>>.........<<<....<<<<...>>>>..>>>..>...>>.>.....
<<<<<<<<<.<<<....>>>..>>>>>>.>>>......<<..<.......
....>..>>.........<<<<<........<<<<........>>>>.>>
>>>..>>>>>>.>>>.........<.<((.....<.<<...<<<.<<...
<<.<<<..>>>>>...<<<<<<.........<<...<.......>.<<<<
.>>>>...>>........>>>..>>>........<.<<.<<<<<<<....
...........>>>>>>>.>>.>....>>....<<<<<<..<...<<<<.
.<<<.<<<<<<<<...<<<......>>>......>>>>>>>>.>>>....
...<<....>>...>>>>..>..>>>.>>>...>>>...>>.>....<<<
<<<<...<...<<<<....>>>>...>>>>>>>>..........<<<.<<
.<<<..<.<<<<<<.<<<<......>>>>>>>>>>.>..>>>...<<..)
)>>...>>.....>>>.>.>.<<<......<<<<....>>>>....>>>.
.)))).]]....<<<<<<<<..<<..<<<<<.<<<<.<<<<<<.......
...<<<<<....<.<<<<<<.......<<.<<<........>>>.>>...
..>>>>>>..>...<<<.<<.<<<<<<.<<<...<<<......>>>...>
>>....<<<...<...<<<....>>>..>.....<<<<<.....>>>>>.
.......>>>...>>.>>>>..>>..>>>....>>>>>...>>.>>>>.>
>>.>...<<.<<<........<.<<<.<<<<....>>>>.>>>....>..
.......>>>.>>......<.....<<<<<..........>>>>>.....
>.....>>>>>.....<<<<<<<<<.....>>>>>>>>>......>>...
>>>>>>>>......<<<...<<....<<<<.<<....<<<<<<<<.<<<.
.<<<<.<<...<<<<<<<<<<<<<<<..>>>>>>>>>.>.>>>>>...>>
.>>>>..>>>.>>>>>>>>...>>.>>>>.....>>.>>><<<<<<<<<.
...>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wtt The nucleoplasmic phase of pre-40S formation prior to nuclear export.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): G2 R5 N7 H9 K10 R11 R12 K13 T14 G16 K19 Y21 H22 K23 K24 R25 K26 Y27 L29 R31 A33 A34 R41 R42 I43 H44 T45 R47 V48 R49 G50 N52 K53 K54 Y55 R56 N64 S66 T73 S86 K98 N99 I145 K147 K148 Y149 A176 R178 Q181 C182 G183 R184
• Binding residue (residue number reindexed from 1): G1 R4 N6 H8 K9 R10 R11 K12 T13 G15 K18 Y20 H21 K22 K23 R24 K25 Y26 L28 R30 A32 A33 R40 R41 I42 H43 T44 R46 V47 R48 G49 N51 K52 K53 Y54 R55 N63 S65 T72 S85 K97 N98 I144 K146 K147 Y148 A175 R177 Q180 C181 G182 R183

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7wtt, PDBe:7wtt, PDBj:7wtt
• PDBsum: 7wtt
• PubMed: 36321656
• UniProt: P62241|RS8_HUMAN Small ribosomal subunit protein eS8 (Gene Name=RPS8)