Structure of PDB 7as9 Chain I Binding Site BS01

Receptor Information

>7as9 Chain I (length=175) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

SRVGKKLLEIPSDVTVTLNDNNTVAVKGPKGELTRTFHPDMEIKVEDNVL
TVARPSDQKEHRALHGTTRSLLGNMVEGVSKGFERGLELVGVGYRASKSG
NKLVLNVGYSHPVEIVPEEGIEIEVPSQTKVVVKGTDKERVGAIAANIRA
VRSPEPYKGKGIRYEGEVVRRKEGK

Ligand information

>7as9 Chain A (length=2812)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaug
aaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuug
auccggagauuuccgaauggggaaacccaccacucguaauggagugguau
ccauaucugaauucauaggauaugagaaggcagacccggggaacugaaac
aucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcgg
cgagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugu
aggacacucuguacggaguuacaaaggaacgagguagaugaagaggucug
gaaaggcccgccauaggagguaacagcccuguagucaaaacuucguucuc
uccugaguggauccugaguacggcggaacacgugaaauuccgucggaauc
cgggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaa
ccaguaccgugagggaaaggugaaaagcaccccggaaggggagugaaaga
gauccugaaaccgugugccuacaaguagucagagcccguuaacgggugau
ggcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguu
aagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcaug
aguacguggucguagacccgaaaccaggugaucuacccauguccagggug
aaguucagguaacacugaauggaggcccgaacccacgcacguugaaaagu
gcggggaugagguguggguaggggugaaaugccaaucgaaccuggagaua
gcugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuu
ggagguagagcacugauuggacuaggggcccggguuaccgaauucaguca
aacuccgaaugccaaugacuuauccuugggagucagacugcgagugauaa
gauccguagucgaaagggaaacagcccagaccgccagcuaaggucccaaa
guauacguuaaguggaaaaggauguggaguugcuuagacaaccaggaugu
uggcuuagaagcagccaccauuuaaagagugcguaauagcucacuggucg
agugacucugcgccgaaaauguaccggggcuaaacguaucaccgaagcug
cggacuguucgaacagugguaggagagcguucuaagggcugugaagccag
accggaaggacugguggagcgcuuagaagugagaaugccgguaugaguag
cgaaagaggggugagaauccccuccaccgaaugccuaagguuuccugagg
aaggcucguccgcucaggguuagucgggaccuaagccgaggccgaaaggc
guaggcgauggacaacagguugauauuccuguaccaccuccucaccauuu
gagcaauggggggacgcaggaggauaggguaagcgcgguauuggauaucc
gcguccaagcaguuaggcugggaaauaggcaaauccguuucccaaaggcu
gagcugugauggcgagcgcgaaguuccugauuccacacugccaagaaaag
ccucuagcgaggugagaggugcccguaccgcaaaccgacacagguaggcg
aggagagaauccuaaggugaucgagagaacucucguuaaggaacucggca
aaaugaccccguaacuucgggagaaggggugcucuguuagggugcaagcc
cgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaacacag
gucucugcgaagccguaaggcgaaguauaggggcugacgccugcccggug
cuggaagguuaagaggagcgcuuagcguaagcgaaggugcgaauugaagc
cccaguaaacggcggccgucgguccuaagguagcgaaauuccuugucggg
uaaguuccgacccgcacgaaaggcgcaacgaucugggcacugucucaacg
agagacucggugaaauuauaguaccugugaagaugcagguuacccgcgac
aggacggaaagaccccguggagcuuuacugcagccugauauugaauguug
guuugaccuucuaacccgccgcccuuaucgggcggggagacagugucagg
ugggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgccc
aaagguucccucagaaugguuggaaaucauucgcagaguguaaaggcaca
agggagcuugacugcgagaccuacaagucgagcagggacgaaagucgggc
uuagugauccggugguuccgcauggaagggccaucgcucaacggauaaaa
gcuaccccggggauaacaggcuuaucucccccaagaguccacaucgacgg
ggagguuuggcaccucgaugucggcucaucgcauccuggggcuguagucg
gucccaaggguugggcuguucgcccauuaaagcgguacgcgagcuggguu
cagaacgucgugagacaguucggucccuauccgucgcgggcgcaggaaau
uugagaggagcuguccuuaguacgagaggaccgggauggacgcaccgcug
guguaccaguuguucugccaagggcaucgcuggguagcuaugugcggacg
ggauaagugcugaaagcaucuaagcaugaagccccccucaagaugagauu
ucccauuccgcaaggaaguaagaucccugaaagaugaucagguugauagg
ucugagguggaaguguggcgacacauggagcugacagauacuaaucgauc
gaggacuuaacc

<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<....
..<<<<<<..[..((.>>>.....<<<<<<<..<<.))..]...>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<...
.>>......>>>>>.>.........<<....>>...<<<<......<<..
...>>.....>>>>...(((....<<<<<<<<....>>>>>>>>......
<<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<..
....>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>
>>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>.....
.<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].........
......<<....>>....]].........<<<<....>>>>.........
....}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>
>>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<.
..<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....
.>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>
>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>.
.>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<
<<.....<<<...<<<<<<<<...<<.<<<..>>>..>>...>>>>>>>>
..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<.....
.>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((...
..<<<<<<...........<<..<<<<<<<<<<.......<<<<<.<<..
.<<<<.......>>>>...>>.........<<......>>...>>>>>..
.>>>>>>>>>>.>>...........))).....>>>>>>.>>>...>>>>
>>><<<<<<..>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<
.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<
<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<.
..<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>
...>>>...>>....<<<<........>>>>.))<<<<..<<<<<<....
..<<...<<<<....<<<<..<<<..<<<.<<<<<<<<...(((....>>
>>>><<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>...>>>>
..>>>>>...>><.........>>>>>>...>>>...>>>>.........
>>>>..>>..>>>>>>.>>>>>>>>..>>.....<<......>>>>>>>.
<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<..........
.....<<<<<...............>>>>><<<<<....<<<<....>>>
>...>>>>>............<<<<<<<<<..<<<.........>>>..<
<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..<<<
<<<<..<<<...<<...<<<<<<.<<....>>..>>>>>>...>>...>>
>>>>>>..>>>>><<<<<.>>>>>........<...........<<<<<<
.....>>>>>>.....>....>>>>...>>.>>>>>>>.....>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<.<<<.
....>>>.>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>
>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<
...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>...
.>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>)
))]......<<<<<<<.((..>>>>..))>>>>>>>.............>
>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>...>>
>>>>...>>>>.>.......<.<<<.<<<<<<..<<<<<<<<<......>
>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>..>..
..<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<
<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<
<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<
<<.<..<<<<<...........>>>>>...>>>>.>>>>.......>>>>
>>>..<<<<....>>>>....<<<<<<<<<.......>>>>>....<<<<
<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>
....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7as9 Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): S2 R3 V4 K6 K60 R63 A64 N75 Y110 S111 H112 K139 E140 G143 Y158 K159 K161 K176
• Binding residue (residue number reindexed from 1): S1 R2 V3 K5 K59 R62 A63 N74 Y109 S110 H111 K138 E139 G142 Y157 K158 K160 K175

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7as9, PDBe:7as9, PDBj:7as9
• PDBsum: 7as9
• PubMed: 33259810
• UniProt: P46898|RL6_BACSU Large ribosomal subunit protein uL6 (Gene Name=rplF)