Structure of PDB 6ras Chain I Binding Site BS01

Receptor Information

>6ras Chain I (length=433) Species: 74545 (Prochlorococcus marinus str. MIT 9302)

ELFKEEIIHQLELHPSRLDKEKIISEAMEDGIDDFFEGIRMALDPLVTFG
VKIVPEKESEKSQNFLWEDFRKLANKLMQRELTGHAARDAILTAMESATK
EEWNGFYRRVLIKDLACGVSEKTINKIAKKFPKYAIPIFSCPLAHDSANH
EKKMIGKKQIEIKLDGVRVLTIIRQNKVEMFSRNGKQFHNFGHIILEIEN
VLKEDPAPYDLVLDGEVMSANFQDLMKQVHRKDGKQTKDAVLHLFDLCPL
ENFQKGRWNTKQTARSLLVKKWVAKHSLLLKHIQTLEWENVDLDTIQGQK
RFVELNKSAVEGGYEGVMIKDPDGMYECKRTHSWLKAKPFIEVTLKVVSV
EEGTGRNKGRLGAILVEGEDDGYEYSLSCGSGFSDIQREEYWSKRKHLLG
QLVEIRADAKTKSKDGVAFSLRFPRFKCFRGFK

Ligand information

>6ras Chain B (length=21)

ttccgacagtggggtcgcaat

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6ras Structural intermediates of a DNA-ligase complex illuminate the role of the catalytic metal ion and mechanism of phosphodiester bond formation.
• Resolution: 2.75 Å
• Binding residue (original residue number in PDB): T87 H89 G122 K126 T127 Q227 M230 K231 R235 K236 G357 T358 R360 N361 G366 A367 S382 T415 K416 S417 K418 S424 L425 F427
• Binding residue (residue number reindexed from 1): T83 H85 G118 K122 T123 Q223 M226 K227 R231 K232 G353 T354 R356 N357 G362 A363 S378 T411 K412 S413 K414 S420 L421 F423

Enzymatic activity

• Enzyme Commision number: 6.5.1.1: DNA ligase (ATP).

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003909 DNA ligase activity
• GO:0003910 DNA ligase (ATP) activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006260 DNA replication
• GO:0006281 DNA repair
• GO:0006310 DNA recombination

External links

• PDB: RCSB:6ras, PDBe:6ras, PDBj:6ras
• PDBsum: 6ras
• PubMed: 31312841
• UniProt: A0A0A2ACP7