Structure of PDB 4wfb Chain I Binding Site BS01

Receptor Information

>4wfb Chain I (length=131) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGGGVRPGF
EGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESG
VVKNEKSGIKILGNGSLDKKLTVKAHKFSAS

Ligand information

>4wfb Chain X (length=2707)

gauuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccaau
gaaggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuu
gauccagagauuuccgaauggggaaacccagcaugaguuaugucauguua
ucgauauguauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuaugaguuacaaaggacgacauuagacgaaucaucuggaaag
augaaucaaagaagguaauaauccuguagucgaaaauguugucucucuug
aguggauccugaguacgacggagcacgugaaauuccgucggaaucuggga
ggaccaucuccuaaggcuaaauacucucuagugaccgauagugaaccagu
accgugagggaaaggugaaaagcaccccggaaggggagugaaauagaacc
ugaaaccgugugcuuacaaguagucagagcccguuaaugggugauggcgu
gccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagca
guaaauguggagccguagcgaaagcgagucugaauagggcguuuaguauu
uggucguagacccgaaaccaggugaucuacccuuggucagguugaaguuc
aggcugaauggaggaccgaaccgacuuacguugaaaagugagcggaugaa
cugaggguagcggagaaauuccaaucgaaccuggagauagcugguucucu
ccgaaauagcuuuagggcuagccucaagugaugauuauuggagguagagc
acuguuuggacgaggggguuaccgaauucagacaaacuccgaaugccaau
aauuuaacuugggagucagaacaugggugauaagguccguguucgaaagg
gaaacagcccagaccaccagcuaaggucccaaaauauauguuaaguggaa
aaggauguggcguugcccagacaacuagcucacuagucggugacacugcg
ccgaaaauguaccggggcuaaacauauuaccgaagcuguggauuguccug
gacaaugguaggagagcguucuaagggcguugaagcaugaucguaaggac
auguggagcgcuuagaagugagaaugccggugugaguagcgaaagacggg
ugagaaucccguccaccgauugacuaagguuuccagaggaaggcucgucc
gcucuggguuagucggguccuaagcugaggccgacaggcguaggcgaugg
auaacagguugauauuccuguaccaccuauaaucguaucgauggggggac
gcaguaggauaggcgagcgugcgauuggaugcacgucuaagcaguaaggc
ugaguaaaauccgguacucgggcugagcugugaugggagaagaagucguu
gauuucacacugccgagaaaagccucuagauagaauaggugcccguaccg
caaaccgacacagguagucaagaugagaauucuaaggugagcgagcgaac
ucucguuaaggaacucggcaaaaugaccccguaacuucgggagaaggggu
gcucuuuaggguuaacgcccagaagagccgcagugaauaggcccaagcga
cuguuuaucaaaaacacaggucucugcuaaaccguaaggugauguauagg
ggcugacgccugcccgugcuggaagguuaagaggagugguuaggcgaagc
uacgaaucgaagccccaguaaacggcggccccuaagguagcgaaauuccu
ugucggguaaguuccgacccgcacgaaaggcguaacgauuugggcacugu
cucaacgagagacucggugaaaucauaguaccugugaagaugcagguuac
ccgcgacaggacggaaagaccccguggagcuuuacuguagccugauauug
aaauucggcuuucuaacccgcaccacuuaucguggugggagacaguguca
agcgggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgc
ucaaagguucccucagaaugguugaaaucauucauagaguguaaaggcau
aagggagcuugacugcgagaccuacaagucgagcagggucgaaagacgga
cuuagugauccggugguuccgcauggaagggccaucgcucaacggauaaa
agcuaccccggggauaacaggcuuaucucccccaagaguucacaucgacg
gggagguuuggcaccucgaugucggcucaucgcauccuggggcuguaguc
ggucccaaggguugggcuguucgcccauuaaagcgguacgcgagcugggu
ucagaacgucgugagacaguucggucccuauccgucgugggcguaggaaa
uuugagaggagcuguccuuaguacgagaggaccgggauggacauaccucu
gguguaccaguugucgugccaacggcauagcuggguagcuauguguggac
gggauaagugcugaaagcaucuaagcaugaagccccccucaagaugagau
uucccaacuuguuauaagaucccucaaagaugaugagguuaauagguucg
agguggaagcauggugacauguggagcugacgaauacuaaucgaucgaag
acuuaau

..<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.
<<<<<<<<<.......>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>.......
<<<<<<<<...<..........<<<<<<<<<.<<<...<<<<<......>
>>>>((......<<<.....))>>>...>>>...>>>>>>>>.>...>.>
>>>>..>>>......<<<<<<<<.........>>>>>>>>......<<<<
<..<<<<>>>>>..)))......]].>>>>>>>.]]..............
.<<....>>....]].........<<<<....>>>>..............
....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>..
.<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<<
.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>
.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<
<<.>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....>
>>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<
<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<.
..<<<<<<<<...<<......>>...>>>>>>>>..>>.>.....>>>>>
>>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>....<<
<......>>>...<<<<.<<<...<<<(((.....<<<<<<.........
..<<..<<<<.<<<<<.......<<<<......>>>>..>>>>>.>>>>.
>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<<.>
>>>>>>....>>>>>>..<<<<<<<.<<......<<<<<.<<....>>.>
>>>>...>>.>>>>>>>........>>>>>><......<<....<<<<<<
.......>>>>>><<<<....<<<<<.<<<..<<<<<<...<<.....>>
.>>>>>>....((<<<<.<<.<.<<<...<<<....>>>...>>>..>>>
...<<<<<........>>>>>))<<<<<<<......<<<...<<<<....
<<<<...<<...<...<.<<....{.{...>>.>.<....<<<<<..<<<
<.<<<..........>>>.>.>>>>..>>>>....>.............>
....>>...>>>>.........>>>.>.>>>...>>>>>>>>>>>.>>>.
....<<......>>>>>>>.<<<<....>.>>>..>>>>..<<<<....<
<<<<<<<.<<.........<.....<<<<<.....<..>......>>>>>
<<<<<<<.<<<<....>>>>.>>>>>>>............<<<<<<<<<.
.<<<.........>>>..<<<<<<<.....<<<....>>>.......>>>
>>>>.<<<<<..<<.<<<<<<<..<<<<..<<...<<<<<<......>>>
>>>...>>..>>>>>>>>>..>>>>..............<..........
.<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..
>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<
<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<.<<<<<<.<
<<<.<..>.>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>
.>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..
<<...<<<<<<<...<<<<<<......>>>>>>.....<<.[.(((>>..
..>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>
)))]......<<<<<<<.((..>>>>..))>>>>>>>.............
>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>...>
>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......
>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>.
...<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<
<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<
<<<...<<<<<<<<<<<.}.}>>>>>>..>>>>>...>>>>.>>>>>...
<<<....<<<<<...........>>>>>....>>>.>>>>.......>>>
>>>>.<<<..>>>....<<<<<<<<<.......>>>>>....<<<<<<<<
.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>....
>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wfb Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.
• Resolution: 3.43 Å
• Binding residue (original residue number in PDB): L3 H4 L6 S12 R13 K14 R16 R18 V19 G20 R21 G22 A24 T30 S31 G32 R33 H35 K36 Q38 K39 A40 R47 P48 G49 F50 E51 Q54 L55 P56 L57 F58 R59 R60 L61 P62 N68 N70 E98 S116
• Binding residue (residue number reindexed from 1): L3 H4 L6 S12 R13 K14 R16 R18 V19 G20 R21 G22 A24 T30 S31 G32 R33 H35 K36 Q38 K39 A40 R47 P48 G49 F50 E51 Q54 L55 P56 L57 F58 R59 R60 L61 P62 N68 N70 E98 S116

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wfb, PDBe:4wfb, PDBj:4wfb
• PDBsum: 4wfb
• PubMed: 26464510
• UniProt: P0A0F8|RL15_STAA8 Large ribosomal subunit protein uL15 (Gene Name=rplO)