Structure of PDB 8wi7 Chain H Binding Site BS01

Receptor Information

>8wi7 Chain H (length=182) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

KALPRLKQRYREEIREALQQEFNYANVMQIPGVVKVVVNMGVGDAARDAK
LINGAINDLALITGQKPEVRRARKSIAQFKLREGMPIGARVTLRGDRMWE
FLDRLISIALPRIRDFRGLSPKQFDGTGNYTFGLNEQSMFHEIDVDSIDR
PRGMDITVVTTATNDAEGRALLRALGFPFKEN

Ligand information

>8wi7 Chain A (length=3025)

aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugagacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaacacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccaugcaugggaccuucguugguaguagucaagcgaugggg
ugacgcaggaagguagccguaccggucagugguaauaccgggguaagccu
guagggagucagauagguaaauccgucuggcauauauccugagaggugau
gcauagccgagugaggcgaauucggugauccuaugcugccgagaaaagcc
ucuagcgaggacauacacggcccguaccccaaaccaacacagguggucag
guagagaauacuaaggcguacgagugaacuaugguuaaggaacucggcaa
aaugcccccguaacuucgggagaagggggacccacauggcguguaagccu
uuacggcccaagcgugaguggguggcacaaaccagugagaagcgacuguu
uacuaaaaacacagguccgugcgaagucgcaagacgauguauacggacug
acgccugcccggugcuggaagguuaagaggacccguuaacucccuuuggg
ggugaagcggagaauuuaagccccaguaaacggcggugguaacuauaacc
auccuaagguagcgaaauuccuugucggguaaguuccgaccugcacgaau
ggcguaacgacuucucaacugucucaaccauagacucggcgaaauugcac
uacgaguaaagaugcucguuacgcgcggcaggacgaaaagaccccgggac
cuucacuacaacuugguauuggugcucgauacgguuuguguaggauaggu
gggagacugugaagcucacacgccaguguggguggagucguuguugaaau
accacucugaucguauugggccucuaaccucggaccguauauccgguuca
gggacagugccugguggguaguuuaacuggggcgguugccuccuaaaaug
uaacggaggcgcccaaagguucccucaaccuggacggcaaucagguguug
aguguaagugcacaagggagcuugacugcgagacggacaugucgagcagg
gacgaaagucgggacuagugauccggcaccucugaguggaaggggugucg
cucaacggauaaaagguaccccggggauaacaggcugaucuuccccaaga
guccauaucgacgggaugguuuggcaccucgaugucggcucgucgcaucc
uggggcuggagcaggucccaaggguugggcuguucgcccauuaaagcggc
acgcgagcuggguuuagaacgucgugagacaguucggucucuauccgccg
cgcgcgucagaagcuugaggaaaccugucccuaguacgagaggaccggga
cggacgaaccucugguauaccaguugucccaccaggggcacggcuggaua
gccacguucggacaggauaaccgcugaaagcaucuaagcgggaaaccucu
uccaagaccaggcuucucacccucuaggagggauaaggccccccgcagac
cacgggauugauagaccagaccuggaagccuaguaauaggugcagggaac
uggcacuaaccggccgaaaacuuac

<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.[[.(((.>>>.....<<<<<<<..<<<)))..]].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<<.........(((((....
.>>>>>>>>>>......<<<<..<.....<<<<<<<))))).>>>>>>><
<<<<<.......<<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>.....>.
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<....>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<.<<......<<<<
....>>>>.......>>.>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
<<<<<<<<<........<......>....>>>>>>>>>.>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<
<<<........>>>>>))<<<<<<<<<<.......<<<<..<<<<<<<<.
.....<<<<......>>>>.....>>>>>>>>....>>>>.<<...<<<<
<...<<<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<<
...<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...
>>...<<<......>>>.....>>>>...>>>...>>>>........>>>
>>..>>....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<
<<<.....>>>>..>>>>..<<<<<...<<<<<<<<.<<.........<.
....<<<<<....<<..>>.....>>>>><<<<<<...<<<<....<<<.
....>>>....>>>>..>>>>>>............<<<<<<<<<..<<<.
........>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.
<<<<<.<<<..<<<<<<<..<<<..<<<....<<<<<.<<<<<<...>>>
>>>..>>>>>....>>>..>>>>>>>>..>>>>><<<<<<.......>>>
>>>........<...........<<<<<<.....>>>>>>.....>....
>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>>>>>>>>.....>.((.
.<<<<<......))>>>>>..<<<<<<<<.<<<.<<......<<<<<<<<
<<..<<<<<.<<<.<<<<<.<<.<<<<<<<<<<<<..............<
<<..<<<......<<<<<<<<....>>>>>>>>...>>>...........
.>>>.....>>>>>>>>>>>>.>>...<<<.<<<<<<......>>>>>>>
>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<.......
....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>....
.<<.[.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.
<<<....>>>.>>)))]......<<<<<<<.<<..>>>>..>>>>>>>>>
.............>>>>..>>>>>>.......<<<<...<.<<<<...<<
.......>>...>>>>.>....>>>.>.......<<<<<.<<<<<<..<<
<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>
..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>
>>>>....<<<<<<<...<<<<..<<<<<<<...............>>>>
>>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...
>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>
>>>.......>>>>>>>..<<<<<...>>>>>....<<<<.<<<<.....
..>>>>.....<<<.<<<<.<<<....<<<<<....>>>>>...>>>..>
>>>..>>>...>>>>....>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8wi7 Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): N44 R75 R78 F84 I92 R95 Q128 D130 N134 T136 L139 G158 D160
• Binding residue (residue number reindexed from 1): N39 R70 R73 F79 I87 R90 Q123 D125 N129 T131 L134 G153 D155

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8wi7, PDBe:8wi7, PDBj:8wi7
• PDBsum: 8wi7
• PubMed: 38245551
• UniProt: A0QSG1|RL5_MYCS2 Large ribosomal subunit protein uL5 (Gene Name=rplE)