Structure of PDB 8fak Chain H Binding Site BS01

Receptor Information
>8fak Chain H (length=643) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
PVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGIVVSVSD
ASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALP
ILLRQGRPAANTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLE
NVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAW
LKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARD
LAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQ
HVLDLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDC
GWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCGSTHLVPVGL
GTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGT
QMLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVAGRAGRAG
KQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIV
RAEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPKRGGRWRW
QILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8fak Replication fork binding triggers structural changes in the PriA helicase that govern DNA replication restart in E. coli.
Resolution3.22 Å
Binding residue
(original residue number in PDB)		R105 E254 I255 S278 T303 R304 S305 N426 R427 R428 A433 H464 H465 V487 G488 R512 T539 Q540 K544 G561 F564 S565 S570
Binding residue
(residue number reindexed from 1)		R104 E165 I166 S189 T214 R215 S216 N337 R338 R339 A344 H375 H376 V398 G399 R423 T450 Q451 K455 G472 F475 S476 S481
Enzymatic activity
Enzyme Commision number 3.6.4.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006269 DNA replication, synthesis of primer
GO:0006270 DNA replication initiation
GO:0006276 plasmid maintenance
GO:0006302 double-strand break repair
GO:0006310 DNA recombination
GO:0010332 response to gamma radiation
GO:0031297 replication fork processing
GO:0032508 DNA duplex unwinding
GO:0046677 response to antibiotic
Cellular Component
GO:1990077 primosome complex
GO:1990158 DnaB-DnaC-DnaT-PriA-PriB complex
GO:1990159 DnaB-DnaC-DnaT-PriA-PriC complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8fak, PDBe:8fak, PDBj:8fak
PDBsum8fak
PubMed37169801
UniProtP17888|PRIA_ECOLI Primosomal protein N' (Gene Name=priA)

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