Structure of PDB 8cwo Chain H Binding Site BS01

Receptor Information
>8cwo Chain H (length=134) Species: 1747 (Cutibacterium acnes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
TMTDPIADMLTRLRNANQAYHDQTSMPHSKIKAGIAGILKSEGYIADYKV
NEPKEGEVGKTLTLTLKYGENRERSIAGVRRISKPGLRVYAKSTALPKVL
GGLGIAIISTSQGLLTDKQAHEKSVGGEVLAYVW
Ligand information
>8cwo Chain A (length=1034) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cauuggagaguuugauccuggcucaggacgaacgcuggcggcgugcuuaa
cacaugcaagucgaacggaaaggcccuuggggugcucgaguggcgaacgg
gugaguaacacgugaguaaccugcccuugacuuugggauaacuucaggaa
acuggggcuaauaccggauaggagcuccugcugcaugguggggguuggaa
aguuucggcgguuggggauggacucgcggcuuaucagcuuguuggugggg
uaguggcuuaccaaggcuuugacggguagccggccugagagggugaccgg
ccacauugggacugagauacggcccagacuccuacgggaggcagcagugg
ggaauauugcacaaugggcggaagccugaugcagcaacgccgcgugcggg
augacggccuucggguuguaaaccgcuuucgccugugacgaagcgugagu
gacgguaauggguaaagaagcaccggcuaacuacgugccagcagccgcgg
ugauacguagggugcgagcguuguccggauuuauugggcguaaagggcuc
guaggugguugaucgcgucggaaguguaaucuuggggcuuaacccugagc
gugcuuucgauacggguugacuugaggaagguaggggagaauggaauucc
ugguggagcgguggaaugcgcagauaucaggaggaacaccaguggcgaag
gcgguucucugggccuuuccugacgcugaggagcgaaagcguggggagcg
aacaggcuuagauacccugguaguccacgcuguaaacgguggguacuagg
ugugggguccauuccacggguuccgugccguagcuaacgcuuuaaguacc
ccgccuggggaguacggccgcaaggcuaaaacucaaaggaauuacacacc
gcccgucaagucaugaaaguugguaacacccgaagccgguggccuaaccg
uugugggggagccgucgaaggugggacuggugauuaggacuaagucguaa
caagguagccguaccggaaggugcggcuggauca
.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<..<<.<<<..>>>...>>>..>>>>>......
<<........<<<<<<<..<<...<<<<<<<<<<<<.....<<<<<<...
.>>>>>>......>>>>.....<<<<<<<<<<....>>>>>>>>>>....
.<<....>>>>>>>>>>..>>>>>>>>>.<<<....<<<..<<<<<<<..
.......>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>>>
>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>>.
........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<<<<.
.....<<<<....>>>>.....>>>>...<<<<<<<......<<....>>
.......>>>>>>...>..<<<<<(((...<<<<<.....<<.)))>>..
.....>>>>>>>>>>..>>>>>>>>>..........<<<((.....<<<<
...<<<.<<<<<<<.<<<<<<<<<<.....<<<<<<<.....>>>>>>>.
..>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<....<<<<
<<<<<..<<<......>>>.....>>>>>>>>>...........<<....
>>.>>>>>>>..>>>>.>>>>...>>>...>>>>...<<<<<<<...<<.
..<<<<.<.....>.>>>>...>>>>>>>>>.........<<<<<<..<<
<<<<<<<<<<.<...>.>>>>>>>>>>>>..<<<..))>>>....>>>>>
>.>>>.<<<......<<<<....>>>>....>>>..)))).]........
<..<<.<.<<<<.<<<..<<<<<<..<<<<....<.<<<<..<<<..<..
....>.>>>.>>>>.>...>>>>..>>>>>>..>>>.>>>>..>.>>...
>.....<<<<<<<<<....>>>>>>>>>......
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8cwo Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanism.
Resolution2.84 Å
Binding residue
(original residue number in PDB)		T2 T4 P6 D9 T12 R13 R15 N16 Q19 H22 S30 K31 V59 K85 P86 G87 R89 Y91 K93 S94 K99 S110 T111 S112 K119 H122 S125 G127 E129
Binding residue
(residue number reindexed from 1)		T1 T3 P5 D8 T11 R12 R14 N15 Q18 H21 S29 K30 V58 K84 P85 G86 R88 Y90 K92 S93 K98 S109 T110 S111 K118 H121 S124 G126 E128
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
Biological Process
GO:0006412 translation
Cellular Component
GO:0005737 cytoplasm
GO:0005840 ribosome
GO:0022627 cytosolic small ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8cwo, PDBe:8cwo, PDBj:8cwo
PDBsum8cwo
PubMed36864821
UniProtQ6A6P0|RS8_CUTAK Small ribosomal subunit protein uS8 (Gene Name=rpsH)

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