Structure of PDB 7ohw Chain H Binding Site BS01

Receptor Information

>7ohw Chain H (length=174) Species: 559292 (Saccharomyces cerevisiae S288C)

KYIQTEQQIEVPEGVTVSIKSRIVKVVGPRGTLTKNLKHIDVTFTKVNNQ
LIKVAVHNGGRKHVAALRTVKSLVDNMITGVTKGYKYKMRYVYAHFPINV
NIFIEVRNFLGDKKIRNVPVRVTIEFKDEIVLSGNSVEDVSQNAADLQQI
CRVRNKDIRKFLDGIYVSHKGFIT

Ligand information

>7ohw Chain 1 (length=1639)

guuugaccucaaaucagguaggaguacccgcugaacuuauaucaauaagc
ggaggaaaagaaaccaaccgggauugccuuaguaacgcgagugaagcggc
aaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaauuug
gagagggcaacuuuggggccguuccuugucuauguuccuuggaacaggac
gucauagagggugagaaucccguguggcgaggagugcgguucuuuguaaa
gugccuucgaagagucgaguuguuugggaaugcagcucuaaguggguggu
uccaucuaaagcuaaauauuggcgagagaccgauagcgaacaaguacagu
gauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacgugaaa
uuguugaaagggaagggcauuugaucagacaugguguuuugugcccucug
cuccuuguggguaggggaaucucgcauuucacugggccagcaucaguuuu
gguggcaggauaaauccauaggaauguagcuugccucgguaaguauuaua
gccugugggaauacugccagcugggacugaggacugcgacguaagucaag
gaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaagg
agucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaauga
acgagguuggggccucgcaagaggugcacaaucgaccgauccugaggaug
gauuugguaagagcauagcuguugggacccgaaagauggugaaaaccauc
uaguagcugguuccugccgaaguuucccucaggauagcagaagcucguau
caguuuuaugaaaaugaagagcuuuuagugggccauuuuugguaagcaga
acuggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacac
gcucaucagacacaaaagguguuaguucaucuagacagccggacgguggc
cauggaagucggaauccgcuaaggaguguguaacaacucaccggccgaau
gaacuagcccugaggauggcgcucaagcguguuaccuauacucuaccguc
aggguugauaugaugcccugacgaguaggcaggcguggaggucagugacg
aagccuagaccguaaggucgggucgaacggccucuagugcagaucuuggu
gguaguagcagcgaaaccacagccaaggaacgguugggcuugggcaguca
agcgcgacauugcuauaccgaagcagaauucgguaauguuaccgcaauag
uaauugaacuuaguacgagaggaacaguucauucggauaauugguuuuug
cggcugucuaucaggcauugccgcgaagcuacauccgcuggauuauggcu
gaacgccucuaagucagaauccaugcuagaacgcggugaugcuccacaca
gauggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggcu
agcaacggugcacuuggcggaaaggccuugggugcuugcuggcgaauugc
aaugcguggggauaaaucauuuguauacgacuuagauguacaacggggua
uuguaagcagcugcugagauuccuuuguugucugauuug

..........................<<<<<<.....<..>......>>>
>>.>.........<<....>>..<<<<<......<<....>>.....>>>
>>..<<<..<........<<.<<<<<<...>>>>>>.>>........>..
..<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......>>>>.
((......<<<.....))>>>....>>>>>>>>>..>>>>>>>....>>>
>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<<<.
.>>>>>>..>>>..................................<<..
..>>...............<<<......>>>...................
...............<<<<<.<<<<<<.....<<<<...<<<..<<<<<<
<<<.....>>>>>>>>>......>>>...>>>>..<<<<<<<.<<<<<<<
<<<<<<<<.....<<<<<<<<<......<<<<<......>>>>>......
.>>>>>>>>..>.>>>>>.>>>>>>>>>>........<<<....>>>...
..>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><<<<<<.
...<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.......
....<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<.......>
>>>.>>>>....>>>>>>.>>>>>>..<<.<..<<<<<<<....>>>>>>
>.....>.>>..<<<<<<....<.<........>.><<<<<<<<<....<
<<.<<<<...>>>>>>>..>>>>>>>.>>..<<<<.<<<.<.....>.>>
>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<
<<.....<<.......<<<.<<<<<<<<<<.......<<<<<.<<....<
<.........>>....>>.........<<......>>...>>>>>...>>
>>>>>>>>>>>......))).>>...>>>>>>.>>>>..>>>>>>><<<<
<<<<<.........>>>>>>>>>....>>>>>><.<<<<<<<<.<<....
..<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>>
<<.....<<.>>....>>....<<<<.......>>>>.....<<<<<...
.<..>....>>>>>.<<<<<<<......>>>>>>>..<<<<<<<<..<<<
<.<.<<<<<<...............>>>>>>.><<<<<...<<<<....<
<<<<<<<.<...>.>>>..>>>>>...>>>>.>>>>>.<<<<...<<<<<
...........>>>>>...>>>>.>>>>....>>>>>>>>.<<<<<<...
...<<..........<<<<<<.....>>>>>>......>>.......<<<
<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>.......
.....>>>>>>.<<<<<<.<..<.......>..>.....<<<<<<<<<.<
<<....<<<..>>>...>>>.>>>.>>>>>>..>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohw Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): K2 S19 I20 K21 S22 R23 I24 V25 K26 H40 D42 V43 T44 V57 H58 R62 K63 A66 T70 S73 L74 N77 H96 F97 D120 K121 K122 R129 E152 S155 Q156 Q163 R168 K170 D171 R173 K174 F175
• Binding residue (residue number reindexed from 1): K1 S18 I19 K20 S21 R22 I23 V24 K25 H39 D41 V42 T43 V56 H57 R61 K62 A65 T69 S72 L73 N76 H95 F96 D112 K113 K114 R121 E138 S141 Q142 Q149 R154 K156 D157 R159 K160 F161

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohw, PDBe:7ohw, PDBj:7ohw
• PDBsum: 7ohw
• PubMed: 34813592
• UniProt: P05738|RL9A_YEAST Large ribosomal subunit protein uL6A (Gene Name=RPL9A)