Structure of PDB 5nd8 Chain H Binding Site BS01

Receptor Information

>5nd8 Chain H (length=164) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

IIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFKQEENTIEVVRPSD
SKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKDLILNV
GYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPY
KGKGIRYQGEYVRR

Ligand information

>5nd8 Chain A (length=2912)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaaugggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuauacggaguuacaaaggacgacauuagacgaaucaucugga
aagaugaaucaaagaagguaauaauccuguagucgaaaauguugucucuc
uugaguggauccugaguacgacggagcacgugaaauuccgucggaaucug
ggaggaccaucuccuaaggcuaaauacucucuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaauaga
accugaaaccgugugcuuacaaguagucagagcccguuaaugggugaugg
cgugccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaa
gcaguaaauguggagccguagcgaaagcgagucugaauagggcguuuagu
auuuggucguagacccgaaaccaggugaucuacccuuggucagguugaag
uucagguaacacugaauggaggaccgaaccgacuuacguugaaaagugag
cggaugaacugaggguagcggagaaauuccaaucgaaccuggagauagcu
gguucucuccgaaauagcuuuagggcuagccucaagugaugauuauugga
gguagagcacuguuuggacgaggggccccucggguaccgaauucagacaa
acuccgaaugccaauuaauuuaacuugggagucagaacaugggugauaag
guccguguucgaaagggaaacagcccagaccaccagcuaaggucccaaaa
uauauguuaaguggaaaaggauguggcguugcccagacaacuaggauguu
ggcuuagaagcagccaucauuuaaagagugcguaauagcucacuagucga
gugacacugcgccgaaaauguaccggggcuaaacauauuaccgaagcugu
ggauuguccuuuggacaaugguaggagagcguucuaagggcguugaagca
ugaucguaaggacauguggagcgcuuagaagugagaaugccggugugagu
cgcgaaagacgggugagaaucccguccaccgauugacuaagguuuccaga
ggaaggcucguccgcucuggguuagucggguccuaagcugaggccgacag
gcguaggcgauggauaacagguugauauuccuguaccaccuauaaucguu
uuaaucgauggggggacgcaguaggauaggcgaagcgugcgauuggauug
cacgucuaagcaguaaggcugaguauuaggcaaauccgguacucguuaag
gcugagcugugauggggagaagacauugagucuucgagucguugauuuca
cacugccgagaaaagccucuagauagaaaauaggugcccguaccgcaaac
cgacacagguagucaagaugagaauucuaaggugagcgagcgaacucucg
uuaaggaacucggcaaaaugaccccguaacuucgggagaaggggugcucu
uuaggguuaacgcccagaagagccgcagugaauaggcccaagcgacuguu
uaucaaaaacacaggucucugcuaaaccguaaggugauguauaggggcug
acgccugcccggugcuggaagguuaagaggagugguuagcuucugcgaag
cuacgaaucgaagccccaguaaacggcggccguaauaacgguccuaaggu
agcgaaauuccuugucggguaaguuccgacccgcacgaaaggcguaacga
uuugggcacugucucaacgagagacucggugaaaucauaguaccugugaa
gaugcagguuacccgcgacaggacggaaagaccccguggagcuuuacugu
agccugauauugaaauucggcacagcuuguacaggauagguaggagccuu
ugaaacgugagcgcuagcuuacguggaggcgcuggugggauacuacccua
gcuguguuggcuuucuaacccgcaccacuuaucguggugggagacagugu
caggcgggcaguuugacuggggcggucgccuccuaaaagguaacggaggc
gcucaaagguucccucagaaugguuggaaaucauucauagaguguaaagg
cauaagggagcuugacugcgagaccuacaagucgagcagggucgaaagac
ggacuuagugauccggugguuccgcauggaagggccaucgcucaacggau
aaaagcuaccccggggauaacaggcuuaucucccccaagaguucacaucg
acggggagguuuggcaccucgaugucggcucaucgcauccuggggcugua
gucggucccaaggguugggcuguucgcccauuaaagcgguacgcgagcug
gguucagaacgucgugagacaguucggucccuauccgucgugggcguagg
aaauuugagaggagcuguccuuaguacgagaggaccgggauggacauacc
ucugguguaccaguugucgugccaacggcauagcuggguagcuaugugug
gacgggauaagugcugaaagcaucuaagcaugaagccccccucaagauga
gauuucccaacuucgguuauaagaucccucaaagaugaugagguuaauag
guucgagguggaagcauggugacauguggagcugacgaauacuaaucgau
cgaagacuuaau

<<<<<<......<<<<<<<<<<<....{[[.(((<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<......>>>>>>>......<
<<<<<<<<.....<<<..........<<<<<<<<.<<<...<<<<<....
..>>>>>((......<<<.....))>>>...>>>...>>>>>>>>...>>
>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.)))..........
....<<....>>....]].........<<<<....>>>>...........
..}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...
<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>
>>>.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<
<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>>
>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......>.
>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.
....<<<...<<<<<<<....<<..<<<...>>>..>>....>>>>>>>.
.>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<<<<......
>>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((....
.<<<<<<...........<<..<<<<.<<<<<.......<<<<<..<...
.<<.........>>....>.......................>>>>>...
>>>>>.>>>>.>>...........))).....>>>>>>.>>>...>>>>>
>><<<<<<<...>>>>>>>....>>>>>>..<<<<<<<.<<......<<<
<<.<<....>>.>>>>>...>>.>>>>>>>........>>>>>><.....
<<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<
<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>
>>...>>>...>>...<<<<<........>>>>>))<<<<<<<.......
...<<<...<<<<....<<<<...<<...<....<.<<......{....>
>.>.<....<<<<...<<<.<<<<<<..<<.....>>.>>>>>>......
>>>...>>>>.....>..........................>....>>.
..>>>>.........>>>>..>>>.....>>>>>>>>>>>..>>.....<
<......>>>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<
<<.<<.........<.....<<<<<....<<..>>.....>>>>><<<<<
<<.<<<<....>>>>.>>>>>>>............<<<<<<<<<..<<<.
........>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.
<<<<<.<<<..<<<<<<<..<<<...<<...<<<<<..<<....>>...>
>>>>...>>...>>>>>>>>..>>>>><<<<<......>>>>>.......
.<...........<<<<<<.....>>>>>>.....>....>>>>>..>>.
>>>>>>>..>..>>.>>>>>>>>.>>>>>>>....>.....<<<<<....
....>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<
.<<<<<<<<<.<<<<.<<<<<<<<<<<...........<<<<<..<<<..
....<<<<<<<<....>>>>>>>>...>>>............>>>>>..>
>>>>>>>>>>.>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>
>>>>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>
..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>
>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>
.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>..........
...>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>>.
..>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<...
...>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.
>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<
<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<.
..<<<<...<<<<<<<<<<<.}..>>>>>>..>>>>>...>>>>.>>>>>
...<<<.<..<<<<<...........>>>>>...>>>>.>>>>.......
>>>>>>>.<<<....>>>....<<<<<<<<<.......>>>>>....<<<
<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>
>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5nd8 Structures and dynamics of hibernating ribosomes from Staphylococcus aureus mediated by intermolecular interactions of HPF.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): K59 T63 G66 T67 A70 G108 Y109 S110 H111 K138 E139 G142 S146 Y157 K158 K160
• Binding residue (residue number reindexed from 1): K52 T56 G59 T60 A63 G101 Y102 S103 H104 K131 E132 G135 S139 Y150 K151 K153

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5nd8, PDBe:5nd8, PDBj:5nd8
• PDBsum: 5nd8
• PubMed: 28645916
• UniProt: Q2FW21|RL6_STAA8 Large ribosomal subunit protein uL6 (Gene Name=rplF)