Structure of PDB 1hr8 Chain H Binding Site BS01

Receptor Information

>1hr8 Chain H (length=441) Species: 4932 (Saccharomyces cerevisiae)

QIPGTRTSKLPNGLTIATEYIPNTSSATVGIFVDAGSRAENVKNNGTAHF
LQHLAFKGTQNRPQQGIELEIENIGSHLNAYTSRENTVYYAKSLQEDIPK
AVDILSDILTKSVLDNSAIERERDVIIRESEEVDKMYDEVVFDHLHEITY
KDQPLGRTILGPIKNIKSITRTDLKDYITKNYKGDRMVLAGAGAVDHEKL
VQYAQKYFGHVPKSESPVPLGSPRGPLPVFCRGERFIKENTLPTTHIAIA
LEGVSWSAPDYFVALATQAIVGNWDRAIGTGTNSPSPLAVAASQNGSLAN
SYMSFSTSYADSGLWGMYIVTDSNEHNVRLIVNEILKEWKRIKSGKISDA
EVNRAKAQLKAALLLSLDGSTAIVEDIGRQVVTTGKRLSPEEVFEQVDKI
TKDDIIMWANYRLQNKPVSMVALGNTSTVPNVSYIEEKLNQ

Ligand information

>1hr8 Chain R (length=7) Species: 559292 (Saccharomyces cerevisiae S288C)

KPATRTL

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1hr8 Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences.
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): Q73 F77 N100 A101 Y102 T103 E160
• Binding residue (residue number reindexed from 1): Q52 F56 N79 A80 Y81 T82 E139

Enzymatic activity

• Catalytic site (original residue number in PDB): H70 Q73 H74 E143 E150
• Catalytic site (residue number reindexed from 1): H49 Q52 H53 E122 E129
• Enzyme Commision number: 3.4.24.64: mitochondrial processing peptidase.

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0005515 protein binding
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0006627 protein processing involved in protein targeting to mitochondrion

Cellular Component

• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0017087 mitochondrial processing peptidase complex

External links

• PDB: RCSB:1hr8, PDBe:1hr8, PDBj:1hr8
• PDBsum: 1hr8
• PubMed: 11470436
• UniProt: P10507|MPPB_YEAST Mitochondrial-processing peptidase subunit beta (Gene Name=MAS1)