Structure of PDB 8ev3 Chain G Binding Site BS01

Receptor Information

>8ev3 Chain G (length=164) Species: 4896 (Schizosaccharomyces pombe)

LKVPPAIAQFQKTLDKNTATQVFKLLNKYRPETAAEKKQRLVAEAEAVAN
GKVSKKPYNVKYGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKK
MGVPYAIVKNKARLGTVIHQKTAAVLAVTEVREEDKNELASIVSAVDANF
SAKYDESRRKWGGG

Ligand information

>8ev3 Chain 1 (length=1452)

uugaccucaaaucagguaggacuacgcgcugaacaauaagcgcaggaaaa
gaaaauaaccaugauucccucaguaacggugaagcgggaaaagcucaaau
uugaaaucuggcaacauuucuuuuguuguccgaguuguaauuucaagaag
cugcuuugaguguagacgaucggucuaaguuccuuggaacaggacgucag
agagggugagaaccccgucuuuggucgauuggauaugccauauaaagcgc
uuucgaagagucgaguuguuugcagcucuaaauggguggucaucuaaagc
uaaauauuggcgagagaccgauagcgaacaaguagagugaucgaaagaug
aaaagaacuuugaaaagagaguuaaauaguacgugaaauugcugaaaggg
aagcauuggaaaucagucuuaccugggugagaucaguagucucucgagac
uaugcacucugaaccugugguaggucagcaucaguuuucgggggcggaaa
aagaauaagggaagguggcuguuuauagcccuuguuguaauacguccacu
ggggacugaggacugcggcuugccaaggaugcugacauaaugguuuucaa
uggcccgucuugaaacacggaccaaggagucuagcaucuaugcgaguguu
ugggugaugaaaacccauccgcgaagugggaacgcccuuguggcgugcac
caucagagcauagcuguugggacccgaaagauggugaaccaucuaguagc
ugguuccugccgaaguuuccggauagcagaaacucagaucaguuuuauga
guauguaaaaugagaguuucuagugggccauuuuugguaagcagaacugg
cgaugcgggaugaaccgaacgugagguuaaggugccggaauguacgcuca
ucagacgaaaagguguuaguucauaugaacuagcccugggcgcuuaagcg
uacuacccauaccucaccgucuggguuagcuuugagaagcucagacgagu
aggcaggcguggagguuugugacgaagccuugggcgugagccugggucga
acagccucuagugcagaucuugguggaaguagcuagaucuagcgaaacca
cagccuggggaacgggccaggcucauaccgaagcagaauucgguaagcuc
gucgcaaugguaauucaacuuaguacgagaggaaccguugauucagauca
uugguauuugcggccgcggagcuaucaucugccggauaacggcugaacgc
cucuaagccagaauccgugccagaaagcgacgauuauguauaaaaauaga
gguaggcuacucuccuguaucguagaagaugggcgaugguugaugaaacg
gaaguguuuuauugacuuguccaugaaauuccaugaauccauugcauacg
acuuuaauguggaacgggguauuguagauuaagccuuuguucccaagauu
ug

........................<<<<<<........>>>>>.>.....
....<<....>>..<<<<<......<<.>>.....>>>>>..<<<..<<.
......<<.<<<<<<<.......>>>>>>>.>>.......>>....<<<<
<.<<<<<..<<<<<..<<<<<<<<<.....<<<<......>>>>.((...
...<<<.....))>>>......>>>>>>>>>..>>>>>.....>>>>>>>
>.>>........<<<<<<<..>>>>>>>.....<<<<<..>>>>>..>>>
..................................<<....>>........
.......<<<<....>>>>...............................
...<<<<<<<<<<<<.....<<<.<<<<.<<<.<<.<<<<<<<..>>>>>
>>>>...>>>..>>>>..>>>..<<<<<<<.<<<<<<<<<<<<<<<....
.....<<<<<<.................>>>>>>........>>>>>.>>
>>>>>>>>........<<<..>>>.....>>>>>>>....>>>>>>>>>>
>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<..
.<<<<.......>>>>...>>>...<<<<..<<<<<.....>>>>>...>
>>>....>>>>>>.>>>>>>..<<.<..<<<<<<<..>>>>>>>.....>
.>>..<<<<<<....<.<<..>>.><<<<<<<<<....<<<.<<<<<<<.
...>>>>>>>>>>.>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>
>....<<<......>>>...<<<<<<<...<<<(((.....<<<<<<...
..<<......<<<.<<<<<<<<<..>>>>>>>>>>>>.))).>>...>>>
>>>.>>>...>>>>>>><<<<<<<<<............>>>>>>>>>...
.>>>>>>..<<<<<<<..<<......<<<<.<<<<....>>>>>>>>...
>>.>>>>>>>........>>>>>><<.....<<<<<..>>>>>....>>.
...<<<<<........>>>>>....<<<<<<<......>>>>>>>...<<
<<<<<..<<<<.<.<<<<<.................>>>>>.><<<<<..
..<<<....<<<..>>>...>>>...>>>>>.<<<<....<<<<......
.....>>>>....>>>>.>>>>....>>>>>>>......<<.......<<
<<..<..>..>>>>....>>........<<<<<....<<<<<<<<<<<<.
.....>>>>>>>>>>>>..>>>>>..........<<<<<.<.........
....>.....<<<<<<<<<.<<<...>>>....>>>>.>>>>>...>>>>
>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8ev3 Chromatin localization of nucleophosmin organizes ribosome biogenesis.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): K105 K128 P129 K133 Y134 G135 L136 N137 H138 A141 K147 V157 P159 L162 K183 A184 K193 A196
• Binding residue (residue number reindexed from 1): K37 K56 P57 K61 Y62 G63 L64 N65 H66 A69 K75 V85 P87 L90 K111 A112 K121 A124

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ev3, PDBe:8ev3, PDBj:8ev3
• PDBsum: 8ev3
• PubMed: 36423630
• UniProt: O13672|RL8_SCHPO Large ribosomal subunit protein eL8 (Gene Name=rpl8)