Structure of PDB 7qwq Chain G Binding Site BS01

Receptor Information

>7qwq Chain G (length=241) Species: 9986 (Oryctolagus cuniculus)

KVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLK
VPPAINQFTQVLDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGK
GDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCR
KMGVPYCILKGKARLCRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTN
YNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

Ligand information

>7qwq Chain 5 (length=3493)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccgguucaacccggcggcggguccggccgugucggcgggucc
cccgaccggcgaccggccgccgccgggcgcauuuccaccgcggcggugcg
ccgcgaccggcuccgggacggcugggaaggcccggcggggaagguggcuc
ggccgaguguuacagcccccccggcagcagcacucgccgaaucccggggc
cgagggagcgagacccgucgccgcgcucuccccccucgggggggccgggc
caccccucccacggcgcgaccgcucggggcggacuguccccagugcgccc
cgggcgggucgcgccgucgggcccgggggaggggccacgcggcgagcgca
cggggucggcggcgacgucggcuacccacccgacccgucuugaaacacgg
accaaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgcc
guggcgcaaugaaggugaaggccggcgcgcucgccggccgaggugggauc
ccgaggccucuccaguccgccgagggcgcaccaccggcccgucucgcccg
ccgcgccggggagguggagcacgagcgcacguguuaggacccgaaagaug
gugaacuaugccugggcagggcgaagccagaggaaacucugguggagguc
cguagcgguccugacgugcaaaucggucguccgaccuggguauaggggcg
aaagacuaaucgaaccaucuaguagcugguucccuccgaaguuucccuca
ggauagcuggcgcucucgcacacgcaguuuuauccgguaaagcgaaugau
uagaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaaaug
gguaagaagcccggcucgcuggcgugagccgggcguggaauggagugccu
agugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaacg
ccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaaggugu
ugguugauauagagccgaaucaacuagcccugaaaauggauggcgcugga
gcgucgggcccauacccggccgucgccggcagucgagaguggacgggagc
ccgcccccggagccccgcggacgcuacgccgcgacgaguaggagggccgc
ugcggugagccuugaagccuagggcgcgggcccggguggagccgccgcag
gugcagaucuuggugguaguagcaaauauucaaacgagaacuuugaaggc
cgaaguggagaaggguuccaugugaacagcaguugaacaugggucagucg
guccugagagaugggcgagcgccguuccgaagggacgggcgauggccucc
guugcccucggccgaucgaaagggagucggguucagauccccgaauccgg
aguggcggagaugggcgccgcgaggcguccagugcgguaacgcgaccgau
cccggagaagccggcgggagccccggggagaguucucuuuucuuugugaa
gggcagggcgcccuggaauggguucgccccgagagaggggcccgugccuu
ggaaagcgucgcgguuccggcggcguccggugagcucucgcuggcccuug
aaaauccgggggagaggguguaaaucucgcgccgggccguacccauaucc
gcagcaggucuccaaggugaacagccucuggcauguuggaacaauguagg
uaagggaagucggcaagccggauccguaacuucgggauaaggauuggcuc
uaagggcugggucggucgucggccggcgccuagcagccgacuuagaacug
gugcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaag
gcccgcggcggguguugacgcgaugugauuucugcccagugcucugaaug
ucaaagugaagaaauucaaugaagcgcggguaaacggcgggaguaacuau
gacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgcau
gaauggaugaacgagauucccacugucccuaccuacuauccagcgaaacc
acagccaagggaacgggcuuggcggaaucagcggggaaagaagacccugu
ugagcuugacucuagucuggcacggugaagagacauguuuuuucacugac
ccggugaggcgggggggcgcucgcuucuggcgccaagcgcccggccgcgc
gccggccgggcgcgacccgcuccggggacagugccagguggggaguuuga
cuggggcgguacaccugucaaacgguaacgcagguguccuaaggcgagcu
cagggaggacagaaaccucccguggagcagaagggcaaaagcucgcuuga
ucuugauuuucaguacgaauacagaccgugaaagcggggccucacgaucc
uucugaccuuuuggguuuuaagcaggaggugucagaaaaguuaccacagg
gauaacuggcuuguggcggccaagcguucauagcgacgucgcuuuuugau
ccuucgaugucggcucuuccuaucauugugaagcagaauucaccaagcgu
uggauuguucacccacuaauagggaacgugagcuggguuuagaccgucgu
gagacagguuaguuuuacccuacugaugauguguuguugccaugguaauc
cugcucaguacgagaggaaccgcagguucagacauuugguguaugugcuu
ggcugaggagccaauggggcgaagcuaccaucugugggauuaugacugaa
cgccucuaagucagaaucccgcccaggcggaacgauacggcagcgccgcg
gagccucgguuggccucggauagccggucccccgcgggguccggugcgga
gugcccuucguccugggaaacggggcgcggccggagaggcggccgccccc
ucgcccgucacgcacgccguucguggggaaccuggcgcuaaaccauucgu
agacgaccugcuucugggucgggguuucguacguagcagagcagcucccu
cgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..[[[....<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>....<<<<<<<<<....<<<<<<<<.<......<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>..>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<..
....<<<<<<<<.....<<<<<<<<<<<...<<<....<<<<<.<....>
>>.>>>......>>>.>>>>>>>>>>>..........>>>.>>>>>..<<
<<<<<..<<<<<<<<<<.<<<..........<<<<.<<<<......<<<<
<..>>>>>.......>>>>.>>>>............>>>...>>>>>>>>
>>....................<<<<<..<<<<<<<.>>>>>>>.<<.<<
<.<<<<..<<<<<<<<<<<<<<<<<<<<<<<.<<<<....>>>>.>>>>>
>>>>>.>>>>>>>>>>>.>>....>.>>>...>>>..>>.....>>>>>.
......>>.>>>>>....>>>...>>>>>>>>..<<<<........>>>>
<<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>...
..>>>...<<....>>...<<<<<<<<....>>>>>>>>..<<<<<...<
<<..<<<...(((..[[.>>>..>>>....>>>>>.<<<<<<<<<.<<<<
......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<..<<<<<
<<.....<<<<<<<<<..<<<<...<<<<<<<....>>>>>>>...>.>>
>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>...<<..
.....>>......>>>>>>...>..>.>>..<<<<<<....<.<<<....
>>>.><<<<<<<<<<............<<<<<<<<<...<<<....<<<.
.<.<<<..<<<<.<.<.....>.>.>>>>>>>.>..>>>..>.>>...>>
>>>>>...<<<<<<<<<..<...>.>>>>>>>>>...>>...>>>>>>>.
>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<
<<<<<<...<<<(((.......<<<<<.....<<..........<<<.<<
<<<<<<<............>>>>>>>>>>>>..........))).>>...
>>>>>..>>>...>>>>>>><<<<<<.<<<...........]].)))...
...........................>>>>>>>>>....>>>>>>...<
<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>
........>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<
<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<
<<<<...<<....<<<....>>>..........<<.<<<<<<<<<...>>
>>>>>>>>>......>>....>>>..<<<<<........>>>>>))<<<<
....<<<....<<<<<<<<....>>>>>>>>..<<<....>>>...<..<
<<<<<<...<<<<<<<<<<<<<<<<<<<<<<<..{{{..>>>>>>....<
<<<<.<<<<.<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>
.....<<<<<<<<....>.>>>>>>>>>>>.>.>>>>>>>>>>>>.....
....>>>>>>>..>.............>>>.>>>>.>>>..>>.....<<
......>>>>>>>.<<<<.....>>>>..>>>....<<<<.....<<<<<
<<.<<.........<.....<<<<<.....<..>......>>>>><<.<<
<<<<<<<<<...<<<<<<.>>>>>>........>>>>>..>>>>>>.>>.
...........<<<<<<<<<..<<<.........>>>..<<<<<<<....
<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....<<
<.....<<<<....>>>>.....>>>.>>>>..>>>>>.<<<<<......
.>>>>>.........<...........<<<<<.......>>>>>.....>
.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>
....<<<<<........>>>>>....[[[[[..{{{.{{......[[[[.
{{[[[[..<<<<<.<<<<<<<<<.<<<<<<<<<<..>>>>>>>>>>...<
<<<<.....<<<<..<<...>>..............<<<<<<<<<<<...
..>>>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<
<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<<<<.
..<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...
<<<..<<<<.......>>>>..>>>.<<<....>>>.>>))).......(
(((((..(((..>>>>>>.)))))))))..........]]]]..}}]]]]
.......<<.<.<.<<<<<<...<<.......>>...>>>>>>...>>.>
.>.......<<<<<.<<<<<<..<.<.<<<<<......>>>>>...>.>.
<<<.........>>>.....>>>>>..>...>>>.>>....<<<<<<<..
..>>>.>>>>....}}..}}}.]]]]].....<<<<<<.<..<<<..<.<
<<<<.................>>>>>.><<<<....<<<<....<<.<<<
<<<<}}}.>>>>>..>>.>>...>>>>...>>>><<<<<...<<<<<...
........>>>>>...>>>>>.>>>...>.>>>>>>......<<<<<<.<
<..<<<.....<<.........<<<<<..<<<<..>>>>.>>>>>.....
...>>....<<..<<<<....<<<<.<<<<<<<......>>>>>>>>>>>
...>>>>..>>..............>>>..>>>>>>>>.<<<<<.<....
.........>....<<.<<<<<<<.<<.....<<<<....<<<<<.....
.>>>>>...>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7qwq Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targeting.
• Resolution: 2.83 Å
• Binding residue (original residue number in PDB): E33 K34 K37 F39 G40 I41 G42 I45 Q46 P47 R49 L51 R53 F54 V55 K56 P58 R59 Y60 I69 L70 K72 R73 K75 E105 K107 Q108 K110 R113 R132 P133 A138 G139 V140 N141 T142 V161 P163 L166 K187 A188 R196 K197 T198 T200 P244 K245
• Binding residue (residue number reindexed from 1): E8 K9 K12 F14 G15 I16 G17 I20 Q21 P22 R24 L26 R28 F29 V30 K31 P33 R34 Y35 I44 L45 K47 R48 K50 E80 K82 Q83 K85 R88 R107 P108 A113 G114 V115 N116 T117 V136 P138 L141 K162 A163 R171 K172 T173 T175 P219 K220

Gene Ontology

Molecular Function

• GO:0003723 RNA binding

Biological Process

• GO:0000470 maturation of LSU-rRNA

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7qwq, PDBe:7qwq, PDBj:7qwq
• PDBsum: 7qwq
• PubMed: 35201867
• UniProt: G1STW0|RL7A_RABIT Large ribosomal subunit protein eL8 (Gene Name=RPL7A)