Structure of PDB 7ope Chain G Binding Site BS01

Receptor Information

>7ope Chain G (length=205) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

PKVALYNQNGSTAGDIELNASVFGIEPNESVVFDAILMQRASLRQGTHKV
KNRSEVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKL
PKKVRRLAIKSVLSSKVIDNNIIVLEDLTLDTAKTKEMAAILKGLSVEKK
ALIVTADANEAVALSARNIPGVTVVEANGINVLDVVNHEKLLITKAAVEK
VEEVL

Ligand information

>7ope Chain A (length=2814)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaug
aaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuug
auccggagauuuccgaauggggaaacccaccacucguaauggagugguau
ccauaucugaauucauaggauaugagaaggcagacccggggaacugaaac
aucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcgg
cgagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugu
aggacacucuguacggaguuacaaaggaacgagguagaugaagaggucug
gaaaggcccgccauaggagguaacagcccuguagucaaaacuucguucuc
uccugaguggauccugaguacggcggaacacgugaaauuccgucggaauc
cgggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaa
ccaguaccgugagggaaaggugaaaagcaccccggaaggggagugaaaga
gauccugaaaccgugugccuacaaguagucagagcccguuaacgggugau
ggcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguu
aagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcaug
aguacguggucguagacccgaaaccaggugaucuacccauguccagggug
aaguucagguaacacugaauggaggcccgaacccacgcacguugaaaagu
gcggggaugagguguggguaggggugaaaugccaaucgaaccuggagaua
gcugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuu
ggagguagagcacugauuggacuaggggcccggguuaccgaauucaguca
aacuccgaaugccaaugacuuauccuugggagucagacugcgagugauaa
gauccguagucgaaagggaaacagcccagaccgccagcuaaggucccaaa
guauacguuaaguggaaaaggauguggaguugcuuagacaaccaggaugu
uggcuuagaagcagccaccauuuaaagagugcguaauagcucacuggucg
agugacucugcgccgaaaauguaccggggcuaaacguaucaccgaagcug
cggacuguucgaacagugguaggagagcguucuaagggcugugaagccag
accggaaggacugguggagcgcuuagaagugagaaugccgguaugaguag
cgaaagaggggugagaauccccuccaccgaaugccuaagguuuccugagg
aaggcucguccgcucaggguuagucgggaccuaagccgaggccgaaaggc
guaggcgauggacaacagguugauauuccuguaccaccuccucaccauuu
gagcaauggggggacgcaggaggauaggguaagcgcgguauuggauaucc
gcguccaagcaguuaggcugggaaauaggcaaauccguuucccaaaggcu
gagcugugauggcgagcgcgaaguuccugauuccacacugccaagaaaag
ccucuagcgaggugagaggugcccguaccgcaaaccgacacagguaggcg
aggagagaauccuaaggugaucgagagaacucucguuaaggaacucggca
aaaugaccccguaacuucgggagaaggggugcucuguuagggugcaagcc
cgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaacacag
gucucugcgaagccguaaggcgaaguauaggggcugacgccugcccggug
cuggaagguuaagaggagcgcuuagcguaagcgaaggugcgaauugaagc
cccaguaaacggcggccguaacgguccuaagguagcgaaauuccuugucg
gguaaguuccgacccgcacgaaaggcgcaacgaucugggcacugucucaa
cgagagacucggugaaauuauaguaccugugaagaugcagguuacccgcg
acaggacggaaagaccccguggagcuuuacugcagccugauauugaaugu
ugguuugaccuucuaacccgccgcccuuaucgggcggggagacaguguca
ggugggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgc
ccaaagguucccucagaaugguuggaaaucauucgcagaguguaaaggca
caagggagcuugacugcgagaccuacaagucgagcagggacgaaagucgg
gcuuagugauccggugguuccgcauggaagggccaucgcucaacggauaa
aagcuaccccggggauaacaggcuuaucucccccaagaguccacaucgac
ggggagguuuggcaccucgaugucggcucaucgcauccuggggcuguagu
cggucccaaggguugggcuguucgcccauuaaagcgguacgcgagcuggg
uucagaacgucgugagacaguucggucccuauccgucgcgggcgcaggaa
auuugagaggagcuguccuuaguacgagaggaccgggauggacgcaccgc
ugguguaccaguuguucugccaagggcaucgcuggguagcuaugugcgga
cgggauaagugcugaaagcaucuaagcaugaagccccccucaagaugaga
uuucccauuccgcaaggaaguaagaucccugaaagaugaucagguugaua
ggucugagguggaaguguggcgacacauggagcugacagauacuaaucga
ucgaggacuuaacc

<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<....
..<<<<<<..[..((.>>>.....<<<<<<<..<<<))..]..>>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<...
.>>......>>>>>.>.........<<....>>...<<<<......<<..
...>>.....>>>>...(((....<<<<<<<<....>>>>>>>>......
<<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<..
....>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>
>>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>.....
.<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].........
......<<....>>....]].........<<<<....>>>>.........
....}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>
>>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<.
..<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....
.>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>
>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>.
.>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<
<<.....<<<...<<<<<<<<...<<<<<<..>>>>.>>...>>>>>>>>
..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<.....
.>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((...
..<<<<<<...........<<..<<<<<<<<<........<<<<<.<<..
.<<<<.......>>>>...>>.........<<......>>...>>>>>..
..>>>>>>>>>.>>...........))).....>>>>>>.>>>...>>>>
>>><<<<<<..>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<
.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<
<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<.
..<<.....>>.>>>>>>....((<<<<.<<.<.<<<...<<<....>>>
...>>>..>>>...<<<<<........>>>>>))<<<<..<<<<<<....
..<<...<<<<....<<<<..<<<..<<<.<<<<<<<<...(((....>>
>>>><<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>...>>>>
..>>>>>...>><..<..>...>>>>>>...>>>...>>>>.........
>>>>..>>..>>>>>>.>>>>>>>>..>>.....<<......>>>>>>>.
<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.........<
.....<<<<<....<....>.....>>>>><<<<<....<<<<....>>>
>...>>>>>............<<<<<<<<<..<<<.........>>>..<
<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..<<<
<<<<..<<<<..<<...<<<<<<.<<....>>..>>>>>>...>>..>>>
>>>>>>..>>>>><<<<<...>>>>>........<...........<<<<
<<.....>>>>>>.....>....>>>>...>>.>>>>>>>..>..>>.>>
>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<
<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<.<<
<<...>>>>.>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>
>>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..
<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>.
...>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>
>)))]......<<<<<<<.((..>>>>..))>>>>>>>............
.>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>>...
>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.....
.>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>
....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<
<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...
<<<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>..
.<<<....<<<<<...........>>>>>....>>>.>>>>.......>>
>>>>>..<<<<....>>>>....<<<<<<<<<.......>>>>>....<<
<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>
>>....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ope RqcH and RqcP catalyze processive poly-alanine synthesis in a reconstituted ribosome-associated quality control system.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): N29 F34 M39 Q40 R41 R45 Q46 T48 V51 K52 N53 R54 S55 V57 R58 G59 R62 K63 Q67 K68 G69 T70 G71 R72 R74 G76 R79 Q82 W83 R84 G85 V88 V89 F90 R95 K100 L101 P102 K103 K104 V105 R106 K135 T136 K137 L165 R168 N169 G180 N182
• Binding residue (residue number reindexed from 1): N28 F33 M38 Q39 R40 R44 Q45 T47 V50 K51 N52 R53 S54 V56 R57 G58 R61 K62 Q66 K67 G68 T69 G70 R71 R73 G75 R78 Q81 W82 R83 G84 V87 V88 F89 R94 K99 L100 P101 K102 K103 V104 R105 K134 T135 K136 L164 R167 N168 G179 N181

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ope, PDBe:7ope, PDBj:7ope
• PDBsum: 7ope
• PubMed: 34255840
• UniProt: P42921|RL4_BACSU Large ribosomal subunit protein uL4 (Gene Name=rplD)