Structure of PDB 7aqc Chain G Binding Site BS01

Receptor Information

>7aqc Chain G (length=175) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

SRVGKKLLEIPSDVTVTLNDNNTVAVKGPKGELTRTFHPDMEIKVEDNVL
TVARPSDQKEHRALHGTTRSLLGNMVEGVSKGFERGLELVGVGYRASKSG
NKLVLNVGYSHPVEIVPEEGIEIEVPSQTKVVVKGTDKERVGAIAANIRA
VRSPEPYKGKGIRYEGEVVRRKEGK

Ligand information

>7aqc Chain A (length=2820)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuuga
uccggagauuuccgaauggggaaacccaccacucguaauggagugguauc
cauaucugaauucauaggauaugagaaggcagacccggggaacugaaaca
ucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcggc
gagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugua
ggacacucuguacggaguuacaaaggaacgagguagaugaagaggucugg
aaaggcccgccauaggagguaacagcccuguagucaaaacuucguucucu
ccugaguggauccugaguacggcggaacacgugaaauuccgucggaaucc
gggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaac
caguaccgugagggaaaggugaaaagcaccccggaaggggagugaaagag
auccugaaaccgugugccuacaaguagucagagcccguuaacgggugaug
gcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguua
agcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcauga
guacguggucguagacccgaaaccaggugaucuacccauguccaggguga
aguucagguaacacugaauggaggcccgaacccacgcacguugaaaagug
cggggaugagguguggguaggggugaaaugccaaucgaaccuggagauag
cugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuug
gagguagagcacugauuggacuaggggccccuaccggguuaccgaauuca
gucaaacuccgaaugccaaugacuuauccuugggagucagacugcgagug
auaagauccguagucgaaagggaaacagcccagaccgccagcuaaggucc
caaaguauacguuaaguggaaaaggauguggaguugcuuagacaaccagg
auguuggcuuagaagcagccaccauuuaaagagugcguaauagcucacug
gucgagugacucugcgccgaaaauguaccggggcuaaacguaucaccgaa
gcugcggacuguucuucgaacagugguaggagagcguucuaagggcugug
aagccagaccggaaggacugguggagcgcuuagaagugagaaugccggua
ugaguagcgaaagaggggugagaauccccuccaccgaaugccuaagguuu
ccugaggaaggcucguccgcucaggguuagucgggaccuaagccgaggcc
gaaaggcguaggcgauggacaacagguugauauuccuguaccaccuccuc
accauuugagcaauggggggacgcaggaggauaggguaagcgcgguauug
gauauccgcguccaagcaguuaggcugggaaauaggcaaauccguuuccc
auaagccugagcugugauggcgagcgaaauauaguagcgaaguuccugau
uccacacugccaagaaaagccucuagcgaggugagaggugcccguaccgc
aaaccgacacagguaggcgaggagagaauccuaaggugaucgagagaacu
cucguuaaggaacucggcaaaaugaccccguaacuucgggagaaggggug
cucuguuagggugcaagcccgagagagccgcagugaauaggcccaggcga
cuguuuagcaaaaacacaggucucugcgaagccguaaggcgaaguauagg
ggcugacgccugcccggugcuggaagguuaagaggagcgcuuagcguaag
cgaaggugcgaauugaagccccaguaaacggcggccguaaacgguccuaa
gguagcgaaauuccuugucggguaaguuccgacccgcacgaaaggcgcaa
cgaucugggcacugucucaacgagagacucggugaaauuauaguaccugu
gaagaugcagguuacccgcgacaggacggaaagaccccguggagcuuuac
ugcagccugauauugaauucuaacccgccgcccuuaucgggcggggagac
agugucaggugggcaguuugacuggggcggucgccuccuaaaagguaacg
gaggcgcccaaagguucccucagaaugguuggaaaucauucgcagagugu
aaaggcacaagggagcuugacugcgagaccuacaagucgagcagggacga
aagucgggcuuagugauccggugguuccgcauggaagggccaucgcucaa
cggauaaaagcuaccccggggauaacaggcuuaucucccccaagagucca
caucgacggggagguuuggcaccucgaugucggcucaucgcauccugggg
cuguagucggucccaaggguugggcuguucgcccauuaaagcgguacgcg
agcuggguucagaacgucgugagacaguucggucccuauccgucgcgggc
gcaggaaauuugagaggagcuguccuuaguacgagaggaccgggauggac
gcaccgcugguguaccaguuguucugccaagggcaucgcuggguagcuau
gugcggacgggauaagugcugaaagcaucuaagcaugaagccccccucaa
gaugagauuucccauuccgcaaggaaguaagaucccugaaagaugaucag
guugauaggucugagguggaaguguggcgacacauggagcugacagauac
uaaucgaucgaggacuuaac

<<<<<<......<<<<<<<<<<<....{[[.(((<<<<<<<[[<<.....
.<<<<<<.....((.>>>.....<<<<<<<..<<<)).....>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<....
>>......>>>>>.>.........<<....>>...<<<<......<<...
..>>.....>>>>...(((....<<<<<<<<<..>>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<...
...>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>>
>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......
<<<<<..<<<<>>>>>..)))......]].>>>>>>>.))).........
.....<<....>>....]].........<<<<....>>>>..........
...}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>
>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<..
.<<<.....>>>...>>>...<<....>>..<<<<.....>>>>......
>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<<..
.<<<<<<......>>>>>>...>.>>>...<<<.<<<<<........>>>
>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..
>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<
<.....<<<...<<<<<<<<...<<.<<<<.....>>>>..>>...>>>>
>>>>..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<.
.....>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<((
(.....<<<<<<...........<<..<<<<<<<<<........<<<<<.
<<...<<<.........>>>...>>.........<<......>>...>>>
>>....>>>>>>>>>.>>...........))).....>>>>>>.>>>...
>>>>>>><<<<<<<...>>>>>>>....>>>>>>..<<<<<<<.<<....
..<<<<<.<<....>>.>>>>>...>>.>>>>>>>........>>>>>><
......<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...
<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<
....>>>...>>>...>>...<<<<<........>>>>>))<<<<<.<<<
<<<......<<...<<<<....<<<<..<<<..<<<.<.<<<.<.....{
....>.>>>.<<..<..<...<<<<.<<<<<<..<<.....>>.>>>>>>
....>>>>....>.>...>><..<<...........>>...>>.>>>...
>>>...>>>>.........>>>>..>>..>>>>>>>>>>>>>>>..>>..
...<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<
<<<<<<.<<<..............<<<<<.....<..>......>>>>><
<<<<....<<<<....>>>>...>>>>>............<<<<<<<<<.
.<<<.........>>>..<<<<<<<.....<<<....>>>.......>>>
>>>>..<<<<.<<<..<<<<<<<..<<<...<<...<<<<<<.<<....>
>..>>>>>>...>>...>>>>>>>>..>>>>><<<<<<..>>>>>>....
....<...........<<<<<<.....>>>>>>.....>....>>>>...
>>.>>>>>>>....>>>.>>>>>>>>.>>>>>>.....>.(...<<<<<.
......)>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<
<<<.<<<<<<<<<.<<<>>>....<<.<<<<<<.....>>>>>>>>....
.>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<...........>
>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[
.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<..
..>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>.....
........>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.....
..>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<
<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>..
.>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>.
...<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<
<<<<...<<<<...<<<<<<<<<<<.}..>>>>>>..>>>>>...>>>>.
>>>>>...<<<....<<<<<...........>>>>>....>>>.>>>>..
.....>>>>>>>..<<<<....>>>>....<<<<<<<<<.......>>>>
>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>
>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7aqc Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC.
• Resolution: 2.99 Å
• Binding residue (original residue number in PDB): S2 V4 K7 R36 H39 K60 R63 A64 G67 T68 S71 N75 Y110 S111 H112 K139 E140 Y158 K173 K176
• Binding residue (residue number reindexed from 1): S1 V3 K6 R35 H38 K59 R62 A63 G66 T67 S70 N74 Y109 S110 H111 K138 E139 Y157 K172 K175

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7aqc, PDBe:7aqc, PDBj:7aqc
• PDBsum: 7aqc
• PubMed: 33259811
• UniProt: P46898|RL6_BACSU Large ribosomal subunit protein uL6 (Gene Name=rplF)