Structure of PDB 6wu9 Chain G Binding Site BS01

Receptor Information

>6wu9 Chain G (length=176) Species: 474186 (Enterococcus faecalis OG1RF)

SRIGNKVVVLPAGVEIKQDGNNITVKGPKGELTREFSSDIKMNIEGNEVT
FTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQVQGN
KLTLNVGYSHPVEMTAPEGVTFEVPANTQVIVKGINKEVVGELAANIRGV
RPPEPYKGKGIRYVGEFVRRKEGKTG

Ligand information

>6wu9 Chain A (length=2739)

uuaagugaauaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacuaacaccgauaugcuuuggggagcuguaaguaagcuauga
uccagagauuuccgaaugggggaacccaauaucuuuuauaggauauuacu
uuucagugaauacauagcugauuagagguagacgcagagaacugaaacau
cuuaguaccugcaggaagagaaagaaaauucgauucccugaguagcggcg
agcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguuguag
gacuccgauaugguagucuguuaguauaguugaaggauuuggaaaauucc
gcuaaagagggugaaagccccguagacgaaaugcugacaacaccuaggag
gauccugaguacggcggaacacgagaaauuccgucggaauccgcggggac
caucccgcaaggcuaaauacucccuagugaccgauagugaaccaguaccg
ugagggaaaggugaaaagcaccccggaaggggagugaaauagauccugaa
accgugugccuacaacaagucaaagcucguuaaugagugauggcgugccu
uuuguagaaugaaccggcgaguuacgauugcaugcgagguuaagucgaag
agacggagccgcagcgaaagcgagucugaauagggcgaaugaguauguag
ucguagacccgaaaccaugugaucuacccauguccagguugaaggugcgg
uaaaacgcacuggaggaccgaacccacguacguugaaaagugcggggaug
agguguggguagcggagaaauuccaaacgaacuuggagauagcugguucu
cuccgaaauagcuuuagggcuagccucggaauugagaaugauggagguag
agcacuguuuggacuaguucagauaaacuccgaaugccauucauuuauau
ccgggagucagacugcgagugauaagauccguagucgaaagggaaacagc
ccagaccaccagcuaaggucccaaaauauauguuaaguggaaaaggaugu
gggguugcacagacaacuaucgagugacccugcgccgaaaauguaccggg
gcuaaacauauuaccgaagcuguggacuacaccauuagguguagugguag
gagagcguucuaagggcguugaaggucgaucgugaggacggcuggagcgc
uuagaagugagaaugccgguaugaguagcgaaagacaggugagaauccug
uccaccguaugacuaagguuuccuggggaaggcucguccgcccaggguua
gucgggaccuaagccgaggccgauaggcguaggcgauggacaacagguug
auauuccuguaccaguuguuuguuugagcaauggagggacgcaguaggcu
aaggaaugcaugcgauuggaagugcauguccaagcaaugagucuugagua
gaguuaaaugcuuuacucuuuaaggacaaguugugacggggagcgaaaua
guagcgaaguuccugacgucacacugccaagaaaagcuucuagugagaaa
acaacugcccguaccguaaaccgacacagguagucgaggagaguauccua
aggugagcgagcgaacucucguuaaggaacucggcaaaaugaccccguaa
cuucgggagaaggggugcugacuucggucagccgcagugaauaggcccaa
gcgacuguuuaucaaaaacacaggucucugcaaaaucguaagaugaagua
uaggggcugacgccugcccggugcuggaagguuaagaggauggguuagcu
ucggcgaagcucagaauugaagccccaguaaacggcggccguaacuauaa
cgguccuaagguagcgaaauuccuugucggguaaguuccgacccgcacga
aaggcguaacgauuugggcacugucucaacgagagacucggugaaauuuu
aguaccugugaagaugcagguuacccgcgacaggacggaaagaccccaug
gagcuuuacuguaguuugauauugaguguuuaacccucuaacccgcacca
cuaaucguggugggagacagugucagaugggcaguuugacuggggcgguc
gccuccuaaaagguaacggaggcgcccaaagguucccucagaaugguugg
aaaucauucgaagaguguaaaggcagaagggagcuugacugcgagaccua
caagucgagcagggacgaaagucgggcuuagugauccggugguuccgcau
ggaagggccaucgcucaacggauaaaagcuacccuggggauaacaggcuu
aucucccccaagaguccacaucgacggggagguuuggcaccucgaugucg
gcucgucgcauccuggggcuguagucggucccaaggguugggcuguucgc
ccauuaaagcggcacgcgagcuggguucagaacgucgugagacaguucgg
ucccuauccgucgcgggcguuggaaauuugagaggagcuguccuuaguac
gagaggaccgggauggacuuaccgcugguguaccaguuguucugccaagg
gcauugcuggguagcuauguagggaagggauaaacgcugaaagcaucuaa
gugugaagcccaccucaagaugagauuucccauuucuuuaagaaaguaag
accccugagagaugaucagguagauagguuggaaguggaaggcuagugau
aguuggagcggaccaauacuaaucggucgaggacuuaac

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
..<<<<<.........>>>>>..>>.>>....<<<<<......<<....>
>.......>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<
<<<<<<...<.<<<.<<.<<<<<<<<<.<<<...<<<.<......>.>>>
((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>>.>>>>
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..)))......]].>>>>>>>.]]...............<<.
...>>....]].........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<.<
........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<<<....
.>>>...>>>...<<....>>..<<<<.....>>>>......>>>>>>>>
>>>>>..<<.<..<<<<.<<.....<<<<<<<<<..<<<....<<<<<<.
.....>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>...
.>>>>>>>>>...<<<.....>>>......>>.>>>...>..>.>>..<<
<<<<....<<<<<....>>>>><<<<<<<<..<..<<<<<<<<<.....<
<<...<<<<<<<<....>>>>>>>>..>>.>.....>>>>.>>>>>..>>
>>>>>.>>..<<<<<<<.<<......>>.>>>>>>>....<<<......>
>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<<
<<<<<<<........<...>....>>>>>>>>>.>>...........)))
.....>>>>>>.>>>...>>>>>>><<<<<<<......>>>>>>>....>
>>>>><.<<<<<<<.<<......<<<<<.<<....>>.>>>>>...>>.>
>>>>>>..>.....>>>>>><......<<....<<<<<<.......>>>>
>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....
((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<<<<...
.....>>>>>))<<<<<<<.........<...<<<<....<<<<..<<<.
.<<<<<...........{...........<<..<<<<<..<<<<.<<<<<
<<<<.....>>>>>>>>>.....>>>>..>>>>>...>>...<<......
...>>....>>>>>...>>>...>>>>.........>>>>..>.......
>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>.
.>>>>..<<<<....<<<<<<<<.<<.........<.....<<<<<....
<<..>>.....>>>>><<<<<<....>>>>>>............<<<<<<
<<<..<<<.........>>>..<<<<<<<.....<<<....>>>......
.>>>>>>>.<<<<<.<<<..<<<<<<<..<<<<..<<...<<<<<..<<.
...>>...>>>>>...>>..>>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<<.....>>>>>>.....>..
..>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>..
...<<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[
[<<<<..<<<<<.<<<<<<<<<.<<<.<<<.>>>.>>>....<<.<<<<<
<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>>
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
...>>>>>>.....<<.[.(((>>....>>>>>>>...<<<<..<<<...
...>>>..>>>>.<<<....>>>.>>)))]......<<<<<<<.((..>>
>>..))>>>>>>>.............>>>>..]]>>>>.......<<<<.
<.<<<<<<...<<.......>>...>>>>>>...>>>>.>.......<<<
<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>
>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>..
..]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<......
.........>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.}..>>>
>>>..>>>>>...>>>>.>>>>>...<<<.<..<<<<<...........>
>>>>...>>>>.>>>>.......>>>>>>>..<<<......>>>.....<
<<<<<<<<.......>>>>>....<<<<<<<<..<<.....<<<<....>
>>>....>>...>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6wu9 Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): R3 I4 K62 T63 G66 T67 A70 K85 Y109 S110 H111 K138 E139 E143 Y157 K158 K160 T176 G177
• Binding residue (residue number reindexed from 1): R2 I3 K61 T62 G65 T66 A69 K84 Y108 S109 H110 K137 E138 E142 Y156 K157 K159 T175 G176

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6wu9, PDBe:6wu9, PDBj:6wu9
• PDBsum: 6wu9
• PubMed: 33004869
• UniProt: Q839E9|RL6_ENTFA Large ribosomal subunit protein uL6 (Gene Name=rplF)