Structure of PDB 8v9l Chain F Binding Site BS01

Receptor Information

>8v9l Chain F (length=182) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

KALPRLKQRYREEIREALQQEFNYANVMQIPGVVKVVVNMGVGDAARDAK
LINGAINDLALITGQKPEVRRARKSIAQFKLREGMPIGARVTLRGDRMWE
FLDRLISIALPRIRDFRGLSPKQFDGTGNYTFGLNEQSMFHEIDVDSIDR
PRGMDITVVTTATNDAEGRALLRALGFPFKEN

Ligand information

>8v9l Chain A (length=3073)

aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugggacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaaccaggagguuggcuuagaagcagccacccuug
aaagagugcguaauagcucacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccgcaugggaccuucguugguaguagucaagcgauggggug
acgcaggaagguagccguaccggucagugguaauaccgggguaagccugu
agggagucagauagguaaauccgucuggcauauauccugagaggugaugc
auagccgagugaggcgaauucggugauccuaugcugccgagaaaagccuc
uagcgaggacauacacggcccguaccccaaaccaacacagguggucaggu
agagaauacuaaggcguacgagugaacuaugguuaaggaacucggcaaaa
ugcccccguaacuucgggagaagggggacccacauggcguguaagccuuu
acggcccaagcgugaguggguggcacaaaccagugagaagcgacuguuua
cuaaaaacacagguccgugcgaagucgcaagacgauguauacggacugac
gccugcccggugcuggaagguuaagaggacccguuaacucccuuuggggg
ugaagcggagaauuuaagccccaguaaacggcggugguaacuauaaccau
ccuaagguagcgaaauuccuugucggguaaguuccgaccugcacgaaugg
cguaacgacuucucaacugucucaaccauagacucggcgaaauugcacua
cgaguaaagaugcucguuacgcgcggcaggacgaaaagaccccgggaccu
ucacuacaacuugguauuggugcucgauacgguuuguguaggauaggugg
gagacugugaagcucacacgccaguguggguggagucguuguugaaauac
cacucugaucguauugggccucuaaccucggaccguauauccgguucagg
gacagugccugguggguaguuuaacuggggcgguugccuccuaaaaugua
acggaggcgcccaaagguucccucaaccuggacggcaaucagguguugag
uguaagugcacaagggagcuugacugcgagacggacaugucgagcaggga
cgaaagucgggacuagugauccggcaccucugaguggaaggggugucgcu
caacggauaaaagguaccccggggauaacaggcugaucuuccccaagagu
ccauaucgacgggaugguuuggcaccucgaugucggcucgucgcauccug
gggcuggagcaggucccaaggguugggcuguucgcccauuaaagcggcac
gcgagcuggguuuagaacgucgugagacaguucggucucuauccgccgcg
cgcgucagaagcuugaggaaaccugucccuaguacgagaggaccgggacg
gacgaaccucugguauaccaguugucccaccaggggcacggcuggauagc
cacguucggacaggauaaccgcugaaagcaucuaagcgggaaaccucuuc
caagaccaggcuucucacccucuaggagggauaaggccccccgcagacca
cgggauugauagaccagaccuggaagccuaguaauaggugcagggaacug
gcacuaaccggccgaaaacuuac

<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.[[.(((.>>>.....<<<<<<<..<<<)))..]].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<<......<..<<<<<.>..
.>>>>>>>>>>......<<<<..[.....<<<<<<<>>>>>.>>>>>>><
<<<<<.......<<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>.....].
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<....>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<<<<....<<<<<.
.....>>>>.>....>>>>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
<<<<<<<<<........<<<<<.<<<..<<<<.......>>>>..>>>..
.......<<......>>...>>>>>....>>>>>>>>>.>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<
<<<........>>>>>))<<<<<<<<<<.......<<<<..<<<<<<<<.
..<..<<<<....>>>>.>...>>>>>>>>....>>>>.<<...<<<<<.
..<<<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<<..
.<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...>>
...<<<......>>>.....>>>>...>>>...>>>>........>>>>>
..>>....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<
<.....>>>>..>>>>..<<<<<...<<<<<<<<.<<.........<...
..<<<<<....<<..>>.....>>>>><<<<<<...<<<<....<<<...
..>>>....>>>>..>>>>>>............<<<<<<<<<..<<<...
......>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<
<<<.<<<..<<<<<<<..<<<<.<<<....<<<<<.<<<<<<...>>>>>
>..>>>>>....>>>.>>>>>>>>>..>>>>><<<<<<.......>>>>>
>........<...........<<<<<<.....>>>>>>.....>....>>
>>>..>>.>>>>>>>..>..>>.>>>>>>>>>>>>>>>.....>.((..<
<<<<......))>>>>>..<<<<<<<<.<<<.<<......<<<<<<<<<<
..<<<<<.<<<.<<<<<.<<.<<<<<<<<<<<<<...........<<<<<
...........<<<<<<<<....>>>>>>>>..................>
>>>>..>>>>>>>>>>>>>.>>...<<<.<<<<<<......>>>>>>>>>
....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<.........
..>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<
<.[.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<
<....>>>.>>)))]......<<<<<<<.<<..>>>>..>>>>>>>>>..
...........>>>>..>>>>>>.......<<<<.<.<.<<<<...<<..
.....>>...>>>>.>...>>>>.>.......<<<<<.<<<<<<..<<<<
<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..
>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>>>
>>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>
>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>>
>>.>>>>>...<<<.<..<<<<<...........>>>>>...>>>>.>>>
>.......>>>>>>>..<<<<<...>>>>>....<<<<.<<<<.......
>>>>.....<<<.<<<<.<<<....<<<<<....>>>>>...>>>..>>>
>..>>>...>>>>....>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8v9l A new family of bacterial ribosome hibernation factors
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): N44 G46 V47 G48 R75 Q83 F84 I92 R95 Q128 F129 D130 T136 L139 N140 G158 D160
• Binding residue (residue number reindexed from 1): N39 G41 V42 G43 R70 Q78 F79 I87 R90 Q123 F124 D125 T131 L134 N135 G153 D155

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8v9l, PDBe:8v9l, PDBj:8v9l
• PDBsum: 8v9l
• PubMed: 38355796
• UniProt: A0QSG1|RL5_MYCS2 Large ribosomal subunit protein uL5 (Gene Name=rplE)