Structure of PDB 8k60 Chain F Binding Site BS01

Receptor Information

>8k60 Chain F (length=315) Species: 100226 (Streptomyces coelicolor A3(2))

DEDDAPAQQVAAAGATADPVKDYLKQIGKVPLLNAEQEVELAKRIEAGLF
AEDKLANSDKLAPKLKRELEIIAEDGRRAKNHLLEANLRLVVSLAKRYTG
RGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQ
ARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAKELDMTPEKVIE
VQKYGREPISLHTPLGEDGDSEFGDLIEDSEAVVPADAVSFTLLQEQLHS
VLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMS
KLRHPSRSQVLRDYL

Ligand information

>8k60 Chain G (length=56)

gagcgttcagcgttcgtttatctccccctggtataatgggagctgtcacg
gatgca

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8k60 Structural insights into transcription activation of the Streptomyces antibiotic regulatory protein, AfsR
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): V215 K216 L219 G223 N282 L285 K317 Y324 K325 S327 T328 Y329 T331 W332 Q336 R340 R350 H354
• Binding residue (residue number reindexed from 1): V20 K21 L24 G28 N87 L90 K122 Y129 K130 S132 T133 Y134 T136 W137 Q141 R145 R155 H159

Gene Ontology

Molecular Function

• GO:0001108 bacterial-type RNA polymerase holo enzyme binding
• GO:0003677 DNA binding
• GO:0005515 protein binding
• GO:0016987 sigma factor activity

Biological Process

• GO:0006352 DNA-templated transcription initiation
• GO:0006355 regulation of DNA-templated transcription
• GO:0010468 regulation of gene expression
• GO:2000142 regulation of DNA-templated transcription initiation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8k60, PDBe:8k60, PDBj:8k60
• PDBsum: 8k60
• PubMed: 39108719
• UniProt: P18183|SIGA_STRCO RNA polymerase principal sigma factor HrdB (Gene Name=hrdB)